scholarly journals MDPFP-FCNN: Multidomain Protein Function Prediction Using Fuzzy Convolutional Neural Network

2021 ◽  
Vol 14 (6) ◽  
pp. 642-655
Keyword(s):  
Neural Network ◽  
Convolutional Neural Network ◽  
Protein Function ◽  
Protein Function Prediction ◽  
Function Prediction ◽  
Multidomain Protein

scholarly journals Prediction of protein function using a deep convolutional neural network ensemble

2017 ◽  
Vol 3 ◽  
pp. e124 ◽  
Author(s):  
Evangelia I. Zacharaki
Keyword(s):  
Neural Network ◽  
Amino Acid ◽  
Convolutional Neural Network ◽  
Protein Function ◽  
Protein Function Prediction ◽  
Function Prediction ◽  
Deep Convolutional Neural Network ◽  
Supervised Machine Learning ◽  
Support Vector ◽  
Homologous Proteins

Background The availability of large databases containing high resolution three-dimensional (3D) models of proteins in conjunction with functional annotation allows the exploitation of advanced supervised machine learning techniques for automatic protein function prediction. Methods In this work, novel shape features are extracted representing protein structure in the form of local (per amino acid) distribution of angles and amino acid distances, respectively. Each of the multi-channel feature maps is introduced into a deep convolutional neural network (CNN) for function prediction and the outputs are fused through support vector machines or a correlation-based k-nearest neighbor classifier. Two different architectures are investigated employing either one CNN per multi-channel feature set, or one CNN per image channel. Results Cross validation experiments on single-functional enzymes (n = 44,661) from the PDB database achieved 90.1% correct classification, demonstrating an improvement over previous results on the same dataset when sequence similarity was not considered. Discussion The automatic prediction of protein function can provide quick annotations on extensive datasets opening the path for relevant applications, such as pharmacological target identification. The proposed method shows promise for structure-based protein function prediction, but sufficient data may not yet be available to properly assess the method’s performance on non-homologous proteins and thus reduce the confounding factor of evolutionary relationships.

scholarly journals ProLanGO: Protein Function Prediction Using Neural Machine Translation Based on a Recurrent Neural Network

Molecules ◽  
2017 ◽  
Vol 22 (10) ◽  
pp. 1732 ◽  
Author(s):  
Renzhi Cao ◽  
Colton Freitas ◽  
Leong Chan ◽  
Miao Sun ◽  
Haiqing Jiang ◽  
...  
Keyword(s):  
Neural Network ◽  
Machine Translation ◽  
Recurrent Neural Network ◽  
Protein Function ◽  
Protein Function Prediction ◽  
Function Prediction ◽  
Neural Machine Translation

scholarly journals Prediction of protein function using a deep convolutional neural network ensemble

2017 ◽  
Author(s):  
Evangelia I Zacharaki
Keyword(s):  
Neural Network ◽  
Amino Acid ◽  
Convolutional Neural Network ◽  
Protein Function ◽  
Protein Structures ◽  
Function Prediction ◽  
Deep Convolutional Neural Network ◽  
Supervised Machine Learning ◽  
Support Vector ◽  
Feature Maps

Background. The availability of large databases containing high resolution three-dimensional (3D) models of proteins in conjunction with functional annotation allows the exploitation of advanced supervised machine learning techniques for automatic protein function prediction. Methods. In this work, novel shape features are extracted representing protein structure in the form of local (per amino acid) distribution of angles and amino acid distances, respectively. Each of the multi-channel feature maps is introduced into a deep convolutional neural network (CNN) for function prediction and the outputs are fused through Support Vector Machines (SVM) or a correlation-based k-nearest neighbor classifier. Two different architectures are investigated employing either one CNN per multi-channel feature set, or one CNN per image channel. Results. Cross validation experiments on enzymes (n = 44,661) from the PDB database achieved 90.1% correct classification demonstrating the effectiveness of the proposed method for automatic function annotation of protein structures. Discussion. The automatic prediction of protein function can provide quick annotations on extensive datasets opening the path for relevant applications, such as pharmacological target identification.

scholarly journals Prediction of protein function using a deep convolutional neural network ensemble

2017 ◽  
Author(s):  
Evangelia I Zacharaki
Keyword(s):  
Neural Network ◽  
Amino Acid ◽  
Convolutional Neural Network ◽  
Protein Function ◽  
Protein Structures ◽  
Function Prediction ◽  
Deep Convolutional Neural Network ◽  
Supervised Machine Learning ◽  
Support Vector ◽  
Feature Maps

Background. The availability of large databases containing high resolution three-dimensional (3D) models of proteins in conjunction with functional annotation allows the exploitation of advanced supervised machine learning techniques for automatic protein function prediction. Methods. In this work, novel shape features are extracted representing protein structure in the form of local (per amino acid) distribution of angles and amino acid distances, respectively. Each of the multi-channel feature maps is introduced into a deep convolutional neural network (CNN) for function prediction and the outputs are fused through Support Vector Machines (SVM) or a correlation-based k-nearest neighbor classifier. Two different architectures are investigated employing either one CNN per multi-channel feature set, or one CNN per image channel. Results. Cross validation experiments on enzymes (n = 44,661) from the PDB database achieved 90.1% correct classification demonstrating the effectiveness of the proposed method for automatic function annotation of protein structures. Discussion. The automatic prediction of protein function can provide quick annotations on extensive datasets opening the path for relevant applications, such as pharmacological target identification.

scholarly journals Neural network and random forest models in protein function prediction

2019 ◽  
Author(s):  
Kai Hakala ◽  
Suwisa Kaewphan ◽  
Jari Björne ◽  
Farrokh Mehryary ◽  
Hans Moen ◽  
...  
Keyword(s):  
Neural Network ◽  
Machine Learning ◽  
Random Forest ◽  
Protein Function ◽  
Protein Function Prediction ◽  
Protein Sequences ◽  
Function Prediction ◽  
Learning System ◽  
Large Set ◽  
Competitive Performance

AbstractOver the past decade, the demand for automated protein function prediction has increased due to the volume of newly sequenced proteins. In this paper, we address the function prediction task by developing an ensemble system automatically assigning Gene Ontology (GO) terms to the given input protein sequence.We develop an ensemble system which combines the GO predictions made by random forest (RF) and neural network (NN) classifiers. Both RF and NN models rely on features derived from BLAST sequence alignments, taxonomy and protein signature analysis tools. In addition, we report on experiments with a NN model that directly analyzes the amino acid sequence as its sole input, using a convolutional layer. The Swiss-Prot database is used as the training and evaluation data.In the CAFA3 evaluation, which relies on experimental verification of the functional predictions, our submitted ensemble model demonstrates competitive performance ranking among top-10 best-performing systems out of over 100 submitted systems. In this paper, we evaluate and further improve the CAFA3-submitted system. Our machine learning models together with the data pre-processing and feature generation tools are publicly available as an open source software athttps://github.com/TurkuNLP/CAFA3Author summaryUnderstanding the role and function of proteins in biological processes is fundamental for new biological discoveries. Whereas modern sequencing methods have led to a rapid growth of protein databases, the function of these sequences is often unknown and expensive to determine experimentally. This has spurred a lot of interest in predictive modelling of protein functions.We develop a machine learning system for annotating protein sequences with functional definitions selected from a vast set of predefined functions. The approach is based on a combination of neural network and random forest classifiers with features covering structural and taxonomic properties and sequence similarity. The system is thoroughly evaluated on a large set of manually curated functional annotations and shows competitive performance in comparison to other suggested approaches. We also analyze the predictions for different functional annotation and taxonomy categories and measure the importance of different features for the task. This analysis reveals that the system is particularly efficient for bacterial protein sequences.

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