scholarly journals Genotypic confirmation of non-broody behaviour of white leghorn by 24bp indel(s) polymorphism at the promoter region of prolactin gene

2020 ◽  
Vol 8 (2) ◽  
pp. 2491-2493
Author(s):  
Azhaguraja Manoharan ◽  
S Sankaralingam ◽  
P Anitha ◽  
Binoj Chacko ◽  
TV Aravindakshan
Keyword(s):  
Promoter Region ◽  
White Leghorn ◽  
Prolactin Gene

​Identification of Single Nucleotide Polymorphism (SNP) C-2161G in the Promoter Region of Prolactin Gene and its Association with Egg Production in Tellicherry Native Chicken

2021 ◽  
Author(s):  
Azhaguraja Manoharan ◽  
S. Sankaralingam ◽  
P. Anitha ◽  
Binoj Chacko ◽  
T.V. Aravindakshan
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Promoter Region ◽  
Egg Production ◽  
Rflp Analysis ◽  
Genotypic Frequency ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Chicken Population ◽  
Native Chicken ◽  
Prolactin Gene

Background: Prolactin is a physiological candidate gene which has significant effects on egg production in poultry. Also, it plays a major role on incubation behaviour (broodiness) in birds. The main objective of the present study was identification of single nucleotide polymorphism (SNP) C-2161G in the promoter region of prolactin gene and its association with egg production in Tellicherry native chicken population. Methods: A total of 200 blood samples were collected from the randomly selected birds of Tellicherry native chicken in All India Co-ordinated Research Project on Poultry improvement (AICRP) farm, Mannuthy, Thrissur, Kerala. Isolation of Genomic DNA was done and the isolated samples were subjected to polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analysis to identify the SNP C-2161G of prolactin gene. Result: On the basis of RFLP patterns, birds were designated with three different genotypes namely CC, CG and GG. The allelic and genotypic frequency was calculated. The observed genotypic frequency at the SNP site C-2161G was CC (0.61), CG (0.160) and GG (0.23) and the frequency of allele was 0.69 for C and 0.31 for G. The egg production was shown to be statistically similar for the genotypes of SNP C-2161G.

Polymorphism of Prolactin gene in relation to egg productionperformance in Kadaknath hens

2016 ◽  
Author(s):  
Shiv Kumar Yadav ◽  
S. K. Maurya ◽  
Alok Kumar Yadav ◽  
Kamalesh Yadav ◽  
K. D. Singh
Keyword(s):  
Body Weight ◽  
Promoter Region ◽  
Egg Production ◽  
Gene Locus ◽  
Production Performance ◽  
Indel Polymorphism ◽  
Native Page ◽  
Wing Vein ◽  
Egg Weight ◽  
Prolactin Gene

Present study was carried out to study egg production performance and polymorphism of Prolactin gene at 24 bp indel locus at promoter region (PRL24). Egg production performances were recorded as age at first egg (AFE), Body Weight at First Egg (WFE), Mean Egg Weight (MEW) and Total No. of Eggs at 90 days of laying (TEN). DNA was isolated from blood of 20 Kadaknath birds collected from wing vein. PRL24 locus for indel polymorphism was amplified by PCR and the product was resolved on 6% native PAGE for genotyping. The AFE (d), WFE (Kg), MEW (g) and TEN of Kadaknath hens in the present study were found to be 188.00±0.71, 1.26±0.03, 42.83±0.21 and 37.75±0.59 respectively. The Prolactin gene locus PRL24 showed two alleles I and D and three genotypes: II, ID and DD. The frequencies of I and D alleles at this locus were 0.55 & 0.45 respectively. The birds of D allele had a significantly (P<0.05) better TEN than birds of I allele.

Dual functions of a cis-acting element within the rat prolactin gene promoter

1989 ◽  
Vol 9 (2) ◽  
pp. 817-819
Author(s):  
E A Barron ◽  
Z Cao ◽  
B G Schneider ◽  
E Kraig ◽  
A J Carrillo ◽  
...  
Keyword(s):  
Promoter Region ◽  
Gene Promoter ◽  
In Vitro Transcription ◽  
Cis Acting ◽  
Transcription System ◽  
Prolactin Gene ◽  
Imperfect Palindrome ◽  
Dual Functions ◽  
Symmetrical Sequence

Within the promoter region of the rat prolactin gene lies a TA-rich imperfect palindrome. The possible functions of the 18-base-pair symmetrical sequence were investigated by using an in vitro transcription system. Prolactin templates with and without the palindrome were transcriptionally assayed in both pituitary and nonpituitary extracts. Our results indicated that the palindromic sequence has at least two functions in the regulation of prolactin transcription.

Genetic polymorphism in the promoter of prolactin gene in layer chicken

2015 ◽  
Vol 49 (6) ◽  
Author(s):  
T. K. Bhattacharya ◽  
R. N. Chatterjee ◽  
U. Raj Kumar ◽  
K. S. Rajaravindra ◽  
M. Niranjan
Keyword(s):  
Genetic Polymorphism ◽  
White Leghorn ◽  
Promoter Fragment ◽  
White Leghorn Chicken ◽  
Layer Chicken ◽  
Prolactin Gene ◽  
Allelic Distribution ◽  
Layer Control

Genetic polymorphism of promoter (Fragment 1, 2 and 3) of prolactin gene in four strains of White Leghorn chicken layers i.e. IWH, IWI, IWK and layer control was identified. Two alleles (A and B) were found in all the promoter fragments of the gene. In above mentioned layer populations, A allele was predominant. The genotypic and allelic distribution was significantly differed among the populations. Different demographic and divergent parameters were estimated in all the lines. It is concluded that all 3 fragments of promoter of prolactin gene was polymorphic in four strains of White Leghorn layer populations.

Identification of 24bp indel(s) Polymorphism in the Promoter Region of prolactin Gene and its Association with Broodiness in Tellicherry Native Chicken

2020 ◽  
Author(s):  
Azhaguraja Manoharan ◽  
S. Sankaralingam ◽  
P. Anitha ◽  
Binoj Chacko ◽  
T. V. Aravindakshan
Keyword(s):  
Promoter Region ◽  
Egg Production ◽  
Indel Polymorphism ◽  
Wing Vein ◽  
Poultry Breeding ◽  
Chicken Population ◽  
Native Chicken ◽  
Incubation Behaviour ◽  
Prolactin Gene ◽  
Reverse Primer

Background: Broodiness is a sex linked behavioural trait observed in most of the domestic fowls and it’s also known as incubation behaviour. Prolactin (PRL) is the principal gene which plays a crucial role to the onset and maintenance of broodiness in birds. The present study was aimed at identification of 24bp (insertion-deletion) indel polymorphism at the promoter region of prolactin gene and its association with broodiness in Telllicherry native chicken population. Methods: A total number of 200 birds of Tellicherry native chicken were randomly selected from All India Co-ordinated Research Project (AICRP) on poultry improvement, Mannuthy, Thrissur, Kerala. Blood samples were collected from the wing vein under aseptic condition and isolation of Genomic DNA was done. Isolated DNA samples were subjected to polymerase chain reaction (PCR) using specific set of forward and reverse primer to detect a 24bp indel polymorphism in the prolactin gene. PCR amplicons were subjected to further molecular analysis. Result: According to the polymorphic patterns birds were categorized to three different genotypes viz., II (insertion-insertion), ID (insertion-deletion) and DD (deletion-deletion). The genotype and allele frequency was calculated and the frequency of I allele (0.6975) was found to be higher than D allele (0.3025). Results of this study suggest that the incubation behaviour could be eliminated in Tellicherry native chicken population by increasing the frequency of I allele upon selective breeding which may enhance the egg production. Therefore, this 24bp indel polymorphism could be used as a molecular marker in poultry breeding.

scholarly journals PCR-RFLP Analysis of Single Nucleotide Polymorphism (SNP) C-2402T at the Promoter Region of Prolactin Gene and its Association with Positively Correlated Production Traits in White Leghorn Chicken

2021 ◽  
Author(s):  
Azhaguraja Manoharan ◽  
S. Sankaralingam ◽  
P. Anitha ◽  
Binoj Chacko ◽  
T.V. Aravindakshan
Keyword(s):  
Venous Blood ◽  
Rflp Analysis ◽  
Production Traits ◽  
White Leghorn ◽  
Coding Region ◽  
Single Nucleotide ◽  
White Leghorn Chicken ◽  
Prolactin Gene ◽  
Pcr Rflp ◽  
Flanking Region

Background: The avian prolactin gene is highly conserved, located on chromosome number 2 and most sequence polymorphisms occurs in the 5’ flanking region, 3’ flanking region, and the coding region of signal peptide. The present study was aimed at the identification of SNP C-2402T of prolactin gene and its association with production traits in White Leghorn chicken. Methods: A total of 200 birds of White Leghorn were selected from All India Co-ordinated Research Project on Poultry improvement (AICRP) farm, Mannuthy. Genomic DNA was isolated from venous blood. Polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analysis was done to identify the SNP C-2402T of prolactin gene. Result: All the birds were observed with the same genotype CC and the frequency of the C allele was one.

scholarly journals Allelic Variation in the Promoter Region of Prolactin Gene in different Population of Native Fowls

2016 ◽  
Vol 7 (13) ◽  
pp. 198-193
Author(s):  
سید عباس نوربخش ◽  
علی هاشمی ◽  
زربخت انصاری پیرسرایی ◽  
نورالدین مرادی ◽  
◽  
...  
Keyword(s):  
Promoter Region ◽  
Allelic Variation ◽  
Prolactin Gene

scholarly journals Dual functions of a cis-acting element within the rat prolactin gene promoter.

1989 ◽  
Vol 9 (2) ◽  
pp. 817-819 ◽  
Author(s):  
E A Barron ◽  
Z Cao ◽  
B G Schneider ◽  
E Kraig ◽  
A J Carrillo ◽  
...  
Keyword(s):  
Promoter Region ◽  
Gene Promoter ◽  
In Vitro Transcription ◽  
Cis Acting ◽  
Transcription System ◽  
Prolactin Gene ◽  
Imperfect Palindrome ◽  
Dual Functions ◽  
Symmetrical Sequence

Within the promoter region of the rat prolactin gene lies a TA-rich imperfect palindrome. The possible functions of the 18-base-pair symmetrical sequence were investigated by using an in vitro transcription system. Prolactin templates with and without the palindrome were transcriptionally assayed in both pituitary and nonpituitary extracts. Our results indicated that the palindromic sequence has at least two functions in the regulation of prolactin transcription.

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