Polymorphism of Prolactin gene in relation to egg productionperformance in Kadaknath hens

2016 ◽  
Author(s):  
Shiv Kumar Yadav ◽  
S. K. Maurya ◽  
Alok Kumar Yadav ◽  
Kamalesh Yadav ◽  
K. D. Singh
Keyword(s):  
Body Weight ◽  
Promoter Region ◽  
Egg Production ◽  
Gene Locus ◽  
Production Performance ◽  
Indel Polymorphism ◽  
Native Page ◽  
Wing Vein ◽  
Egg Weight ◽  
Prolactin Gene

Present study was carried out to study egg production performance and polymorphism of Prolactin gene at 24 bp indel locus at promoter region (PRL24). Egg production performances were recorded as age at first egg (AFE), Body Weight at First Egg (WFE), Mean Egg Weight (MEW) and Total No. of Eggs at 90 days of laying (TEN). DNA was isolated from blood of 20 Kadaknath birds collected from wing vein. PRL24 locus for indel polymorphism was amplified by PCR and the product was resolved on 6% native PAGE for genotyping. The AFE (d), WFE (Kg), MEW (g) and TEN of Kadaknath hens in the present study were found to be 188.00±0.71, 1.26±0.03, 42.83±0.21 and 37.75±0.59 respectively. The Prolactin gene locus PRL24 showed two alleles I and D and three genotypes: II, ID and DD. The frequencies of I and D alleles at this locus were 0.55 & 0.45 respectively. The birds of D allele had a significantly (P<0.05) better TEN than birds of I allele.

Identification of 24bp indel(s) Polymorphism in the Promoter Region of prolactin Gene and its Association with Broodiness in Tellicherry Native Chicken

2020 ◽  
Author(s):  
Azhaguraja Manoharan ◽  
S. Sankaralingam ◽  
P. Anitha ◽  
Binoj Chacko ◽  
T. V. Aravindakshan
Keyword(s):  
Promoter Region ◽  
Egg Production ◽  
Indel Polymorphism ◽  
Wing Vein ◽  
Poultry Breeding ◽  
Chicken Population ◽  
Native Chicken ◽  
Incubation Behaviour ◽  
Prolactin Gene ◽  
Reverse Primer

Background: Broodiness is a sex linked behavioural trait observed in most of the domestic fowls and it’s also known as incubation behaviour. Prolactin (PRL) is the principal gene which plays a crucial role to the onset and maintenance of broodiness in birds. The present study was aimed at identification of 24bp (insertion-deletion) indel polymorphism at the promoter region of prolactin gene and its association with broodiness in Telllicherry native chicken population. Methods: A total number of 200 birds of Tellicherry native chicken were randomly selected from All India Co-ordinated Research Project (AICRP) on poultry improvement, Mannuthy, Thrissur, Kerala. Blood samples were collected from the wing vein under aseptic condition and isolation of Genomic DNA was done. Isolated DNA samples were subjected to polymerase chain reaction (PCR) using specific set of forward and reverse primer to detect a 24bp indel polymorphism in the prolactin gene. PCR amplicons were subjected to further molecular analysis. Result: According to the polymorphic patterns birds were categorized to three different genotypes viz., II (insertion-insertion), ID (insertion-deletion) and DD (deletion-deletion). The genotype and allele frequency was calculated and the frequency of I allele (0.6975) was found to be higher than D allele (0.3025). Results of this study suggest that the incubation behaviour could be eliminated in Tellicherry native chicken population by increasing the frequency of I allele upon selective breeding which may enhance the egg production. Therefore, this 24bp indel polymorphism could be used as a molecular marker in poultry breeding.

​Identification of Single Nucleotide Polymorphism (SNP) C-2161G in the Promoter Region of Prolactin Gene and its Association with Egg Production in Tellicherry Native Chicken

2021 ◽  
Author(s):  
Azhaguraja Manoharan ◽  
S. Sankaralingam ◽  
P. Anitha ◽  
Binoj Chacko ◽  
T.V. Aravindakshan
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Promoter Region ◽  
Egg Production ◽  
Rflp Analysis ◽  
Genotypic Frequency ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Chicken Population ◽  
Native Chicken ◽  
Prolactin Gene

Background: Prolactin is a physiological candidate gene which has significant effects on egg production in poultry. Also, it plays a major role on incubation behaviour (broodiness) in birds. The main objective of the present study was identification of single nucleotide polymorphism (SNP) C-2161G in the promoter region of prolactin gene and its association with egg production in Tellicherry native chicken population. Methods: A total of 200 blood samples were collected from the randomly selected birds of Tellicherry native chicken in All India Co-ordinated Research Project on Poultry improvement (AICRP) farm, Mannuthy, Thrissur, Kerala. Isolation of Genomic DNA was done and the isolated samples were subjected to polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analysis to identify the SNP C-2161G of prolactin gene. Result: On the basis of RFLP patterns, birds were designated with three different genotypes namely CC, CG and GG. The allelic and genotypic frequency was calculated. The observed genotypic frequency at the SNP site C-2161G was CC (0.61), CG (0.160) and GG (0.23) and the frequency of allele was 0.69 for C and 0.31 for G. The egg production was shown to be statistically similar for the genotypes of SNP C-2161G.

scholarly journals SAPU-SAPU FISH (Hyposarcus pardalis) AS A SINGLE PROTEIN SOURCE FOR LAYING MOJOSARI DUCKS

2016 ◽  
Vol 41 (3) ◽  
pp. 117-124 ◽  
Author(s):  
B. Indarsih ◽  
A. Asnawi ◽  
D. K. Purnamasari
Keyword(s):  
Body Weight ◽  
Feed Intake ◽  
Egg Production ◽  
Egg Quality ◽  
Production Performance ◽  
Protein Source ◽  
Egg Mass ◽  
Final Body Weight ◽  
Egg Number ◽  
Single Protein

The present study was conducted to evaluate the optimum level of dietary inclusion and the form of feeding sapu-sapu fish (SSF) as a single protein source for feed components of small holder Mojosari duck farming. A total of 180 twenty four wk-old laying ducks were fed with two forms (sun-dried and ground-fresh) and three levels of SSF (10, 20 and 30%) from 24 to 32 wk of age. Experimental diets were formulated containing 105.1; 141.5 and 177.9 g/kg crude protein and 3078; 3065 and 3052 kcal metabolizable energy (ME) /kg diet. Production performance and egg quality were measured. The form of SSF had a significant effect (P<0.0001) on feed intake, egg production, feed conversion, egg mass and egg number except final body weight. No significant effects (P>0.05) were found on all of the performance parameters measured due to feeding levels of SSF except feed intake and final body weight. The interaction of form and feeding level was significant (P<0.05) on egg and body weights. Low performance and egg quality were observed in the birds fed a diet containing the dried SSF. It can be suggested that local ducks required 20% SSF as a single protein source for maximum egg production (41.2%) and total egg number per wk (3.1 eggs/bird/wk) during 8 wk of rearing period. Egg quality can be improved by feeding the fresh SSF. However, due to be sufficiently better processing technique, the dried SSF would be potential to replace the common fish for laying ducks.

scholarly journals Penggunaan Tepung Limbah Udang sebagai Bahan Pakan Sumber Protein terhadap Performa Produksi Puyuh Fase Layer (Coturnix-coturnix japonica)

2018 ◽  
Vol 5 (2) ◽  
pp. 31
Author(s):  
Hamdan Has ◽  
Astriana Napirah ◽  
Widhi Kurniawan ◽  
Natsir Sandiah
Keyword(s):  
Egg Production ◽  
Production Performance ◽  
Feed Consumption ◽  
Shrimp Waste ◽  
Feed Conversion ◽  
Treatment Unit ◽  
Egg Weight ◽  
Randomized Design ◽  
Average Production ◽  
Laying Quail

ABSTRAKLimbah udang merupakan limbah pengolahan udang yang memiliki potensi sebagai pakan sumber protein bagi ternak puyuh. Penelitian ini bertujuan mengkaji penggunaan tepung limbah udang (TLU) sebagai sumber protein pakan pada puyuh fase layer. Penelitian ini menggunakan rancangan acak lengkap empat perlakuan lima ulangan perlakuan yang digunakan terdiri dari empat level penggunaan tepung limbah udang dalam ransum yaitu P0 (kontrol), P1 (5% TLU), P2 (7,5% TLU) dan P3 (10% TLU), tiap unit perlakuan disi dengan 5 ekor puyuh. Puyuh yang diguanakan adalah puyuh fase layer umur 20 minggu, sebanyak 100 ekor yang didistribusikan kedalam 20 unit percobaan. Bahan pakan yang digunakan adalah jagung, dedak padi, konsentrat petelur dan tepung limbah udang. Data yang diperoleh dianalisis ragam dan data yang berbeda nyata (P<0,05) diuji lanjut menggunakan uji duncan. Variabel yang diamati adalah performa produksi: konsumsi pakan, produksi telur, bobot telur dan konversi ransum. Hasil penelitian menunjukkan bahwa penggunaan TLU dalam ransum (P1,P2 dan P3) menunjukkan pengaruh nyata (P<0,05) dibanding kontrol pada minggu ke-tiga penelitian terhadap bobot telur dan konversi ransum, penggunaan TLU (P1,P2,P3) selama lima minggu meningkatkan konsumsi ransum (P<0,05) dibanding kontrol tetapi tidak berbeda nyata (P>0,05) terhadap produksi telur, bobot telur dan konversi ransum. Kesimpulan penelitian ini bahwa penggunaan TLU dalam ransum dapat digunakan hingga level 7,5% sedangkan level 10% menunjukkan adanya penurunan rata-rata performa produksi.Kata kunci: tepung limbah udang, puyuh fase layer, performa produksiABSTRACTShrimp waste was shrimp processing waste which has the potential as protein source for quail feed. This study was aimed to examine the use of shrimp waste flour (SWF) asprotein source for laying quail feed. This study used  completely randomized design that consist of four treatments and five replications.The treatmentswere using levels of shrimp waste flour in feed and consist of P0 (control), P1 (5% SWF), P2 (7.5% SWF) and P3 (10% SWF ). Each treatment unit was filled with 5 quails. One hundred of 20 weeks laying quails were used in this study. Self mixing feed that contained corn, rice bran, laying concentrate and shrimp waste flour were used in this study. The data obtained were analyzed using analyze of variance and continued using Duncan multiple range test. The variables observed were production performance that consist of feed consumption, egg production, egg weight and feed conversionratio. The results showed that the use of SWF in feed (P1, P2 and P3) showed a significant effect (P <0.05) compared to controls in the third week of research on egg weight and feed conversion ratio.The use of SWF (P1, P2, P3) for five weeks increased feed consumption (P <0.05) compared to controls but not significantly different (P>0.05) for egg production, egg weight and feed conversion. The conclusion of this study was the use of SWF in feed can be used until 7.5% on laying quail feed while the level of 10% indicates a decrease in average production performance.Keywords: shrimp waste flour, laying quail, production performance

scholarly journals Effects on egg production and quality of supplementing drinking water with calcium and magnesium

2021 ◽  
Vol 51 (4) ◽  
pp. 469-476
Author(s):  
X.J. Yi ◽  
A. Rehman ◽  
R.W. Akhtar ◽  
A. Abbas ◽  
K. Hussain ◽  
...  
Keyword(s):  
Drinking Water ◽  
Egg Production ◽  
Laying Hens ◽  
Egg Quality ◽  
Production Performance ◽  
Egg Weight ◽  
Calcium And Magnesium ◽  
Eggshell Colour ◽  
Haugh Unit

This study was conducted to appraise the effects on egg quality and production performance of laying hens when drinking water was supplemented with calcium (Ca) and magnesium (Mg). A total of 384 (64-week-old) Hy-line Brown laying hens were assigned at random to four treatments, which consisted of CON: unsupplemented drinking water; T1: drinking water + 2 mg/L Ca + 250 mg/L Mg; T2: drinking water + 4 mg/L Ca + 510 mg/L Mg /10 L; and T3: drinking water + 5 mg/L Ca and 760 mg/L Mg. The experiment lasted six weeks. Water intake increased linearly in week 1 with the rising levels of Ca and Mg in the drinking water. Increasing the Ca and Mg levels improved eggshell strength (week 2 (P =0.01), week 5 (P =0.01), and week 6 (P = 0.03), and eggshell thickness (week 6) (P =0.02) and reduced the rate at which eggs were broken (week 4) (P =0.01). The supplemental Ca and Mg did not affect egg production, egg weight, Haugh unit, albumen height, eggshell colour, and yolk colour compared with CON. Nor did they influence the Haugh unit and albumen height after storing for 1, 5, 10 and 15 days. In conclusion, adding Ca and Mg to the drinking water increased the thickness and strength of the eggshells.

scholarly journals Breeding for the improvement of indigenous chickens in Bangladesh: performance of second generation

2017 ◽  
Vol 3 (1) ◽  
pp. 66-71
Author(s):  
Shakila Faruque ◽  
AKFH Bhuiyan ◽  
Md Yousuf Ali ◽  
MSK Sarker ◽  
Ziaul Faruque Joy
Keyword(s):  
Body Weight ◽  
Egg Production ◽  
Second Generation ◽  
Breeding Value ◽  
Genotype 3 ◽  
Indigenous Chicken ◽  
Egg Weight ◽  
Slaughter Age ◽  
Dressing Percentage ◽  
Egg Production Rate

The improvement of productivity of indigenous chicken is a long desire in the country. Present research is a part of the long-term selection program being undertaken to evaluate the carcass characteristics and expected response to selection of second generation (G2) of indigenous chicken under intensive management in Bangladesh. A total of 1643-day-old chicks comprising of 3 types of chicken namely Naked Neck (NN), Hilly (H) and Non-descript Desi (ND) were hatched in a two batches for this study. In second generation (G2), selection was practiced at 3 (three) stages of birds life, firstly and secondly at 8 and at 16 weeks of age, selection was performed on the basis of breeding value for 8 and 16 week body weight. Thirdly, at 40-week of age, on the basis of an index comprising the parameters of age at sexual maturity (ASM), body weight (BW), egg production (EP) and egg weight (EW). Improvement target of egg weight was to increase by 1g and improvement target of egg production rate was to increase by 2 % per generation. The main target was the mean body weight in H, ND and NN chicken has to be gone up from the initial eight-week body weight of 375, 342 and 331g to 500 g at eight weeks of age. At 8, 10 and 12 weeks of age, six birds from each genotype were slaughtered to analyze the meat yield traits. Slaughter data were analyzed in a 3(genotype)×3(slaughter age) factorial arrangement in CRD by General Linear Model (GLM) Univariate Procedure in SPSS Computer Program. Genotype and slaughter age had significant effect (p<0.001) on dressing percentage. Body weight at 8 weeks of age was expected to improve by 51.21 vs. 24.03; 37.74 vs. 15.47 and 26.26 vs. 9.65g; respectively for ND, H and NN males and females. In terms of body weight H genotype was superior and NN genotype was for dressing percentage. As a result of selection; EP, BW increased and ASM reduced in second generation than that of the foundation stock.Asian J. Med. Biol. Res. March 2017, 3(1): 66-71

SUBSTITUTION OF SORGHUM FOR WHEAT IN DIETS FOR LAYING HENS

1982 ◽  
Vol 62 (1) ◽  
pp. 305-306 ◽  
Author(s):  
E. E. GARDINER ◽  
D. J. MAJOR ◽  
S. DUBETZ
Keyword(s):  
Body Weight ◽  
Key Words ◽  
Egg Production ◽  
Laying Hens ◽  
Feed Consumption ◽  
White Leghorn ◽  
Egg Weight ◽  
Single Comb

The effects of substituting various levels of sorghum for wheat in diets for laying hens were studied. Egg production, egg weight, feed consumption, body weight and hatchability of eggs from Single Comb White Leghorn hens were not affected by the proportion of sorghum in the diet. Key words: Sorghum, wheat, nutrition, egg production

Comparative performance of Vanaraja, Srinidhi and Desi chicken under traditional system among tribal community of Assam

2018 ◽  
Author(s):  
M. Sarma ◽  
R. Islam ◽  
M. K. Borah ◽  
P. Sharma ◽  
J. D. Mahanta ◽  
...  
Keyword(s):  
Body Weight ◽  
Mortality Rate ◽  
Field Condition ◽  
Egg Production ◽  
Reproductive Traits ◽  
Tribal Community ◽  
Comparative Performance ◽  
Egg Weight ◽  
Traditional System

A study was conducted covering a total of one hundred chicken farmers in tribal dominated Boko Block of Kamrup district in Assam to assess different productive and reproductive traits of Desi, Vanaraja and Srinidhi birds under field condition. Information was obtained on mean body weight at various ages, age at first egg, annual egg production, fertility and hatchability and mortality rate. Body weight, egg production and egg weight were significantly (P£0.05) higher in Vanaraja and Srinidhi birds compared to Desi chicken. The age at first egg was significantly (P£0.05) higher in Desi chicken while compared with Vanaraja and Srinidhi under traditional system of management. However no significant (P£0.05) difference was found between Vanaraja and Srinidhi in their body weight and egg production at various ages. Higher mortality percent in Vanaraja (12.23±1.62) and Srinidhi (11.34±1.23) were recorded during 0 to 5 week. No significant (P£0.05) differences were found among all three groups of birds in fertility and hatchability.

Effect of feeding different levels of lysine and protein on the performance of WLH layers

2017 ◽  
Author(s):  
Naga Raja Kumari K ◽  
Ravinder Reddy V ◽  
Chinni Preetham V ◽  
Srinivas Kumar ◽  
D.Sen A.R. ◽  
...  
Keyword(s):  
Body Weight ◽  
Feed Intake ◽  
Feed Efficiency ◽  
Egg Production ◽  
Egg Mass ◽  
Egg Weight ◽  
Protein Levels ◽  
Lysine Concentration ◽  
Different Levels ◽  
Effect Of Feeding

A trial was conducted to evaluate the requirement of digestible lysine at various protein levels in the diet of WLH layers (BV-300) from 25-44 weeks of age. Layers (528) were fed with diets containing two protein levels i.e. 13.36 and 15.78 % each with 5 % concentration variations of lysine (0.50, 0.55, 0.60, 0.65, and 0.70) and a control with 17 % CP and 0.70 % lysine. Each diet was fed to six replicates of eight birds. Egg production, feed intake, body weight were not influenced either by the concentration of lysine or by level of protein in diet. Increased (P d” 0.05) egg weight and egg mass were observed with increasing lysine in diets. Better feed efficiency was observed with increasing lysine concentration. It can be concluded that WLH layers require approximately 0.65% lysine with 13.36% CP or 0.63% lysine with 15.78% CP (i.e. 598.80 vs 570 mg/h/day) in diet.

Effect of methionine and lysine supplementation of diets for laying hens, on production and feed intake

1976 ◽  
Vol 27 (5) ◽  
pp. 739 ◽  
Author(s):  
MW McDonald ◽  
IJ Bruce
Keyword(s):  
Body Weight ◽  
Feed Intake ◽  
Egg Production ◽  
Function Analysis ◽  
Basal Diet ◽  
Metabolizable Energy ◽  
Feed Consumption ◽  
Multiple Regression Equation ◽  
Weight Changes ◽  
Egg Weight

Six diets containing five levels of methionine and two of lysme were each fed to 12 Leghorn and 12 Australorp pullets for a period of 16 weeks Body weight changes, egg production, egg weight and feed intake were measured Although responses to the diets did not differ significantly In univariate analyses, multrvarrate analysis lndicated a srgnificant interaction between breeds and diets. Increased methionine produced small, significant Increases In egg weight, although responses were inconsistent at different levels Body weight gains were not significantly different between diets. Egg production and feed consumption were significantly affected by diet?, but the pattern of response was also inconsistent. A discriminate function analysis showed a consistent response to increased methionine, which indicated that the basal diet was deficient and that the pullets required a total of 0 275% methionine in their diet Lysine supplementation produced a 'significant decrease In feed intake but had no significant effect on any other variable.A multiple regression equation relating metabolizable energy intake to the variables measured in the experiment was calculated and compared with others reported In the literature This was used to predict the requirements of the pullets for a number of essential amino acids.

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