scholarly journals Identification and characterisation of female released sex pheromone components of jute semilooper, Anomis sabulifera Guenee (Lepidoptera: Noctuidae)

2021 ◽  
Vol 42 (2) ◽  
pp. 254-264
Author(s):  
V.R. Babu ◽  
◽  
S. Satpathy ◽  
B.V.S. Reddy ◽  
◽  
...  

Aim: Identification and characterization of female released sex pheromone components of jute semilooper, Anomis sabulifera Guenee (Lepidoptera: Noctuidae) from female pheromone gland extracts. Methodology: Electroantennogram (EAG) was carried for studying the antennal response; Gas Chromatography coupled with Electro antenna Detector (GC-EAD) was conducted for studying the antennal response of eluted compounds from female pheromone gland extract; Gas Chromatography and Mas Spectrophotometry (GC-MS) was conducted for characterization or getting complete profile of compounds present in the female pheromone gland extract based on retention times. Wind tunnel assay was conducted for studying the behavioural responses of eluted compounds from the female pheromone gland extract. Results: GC-MS profile of female pheromone gland extract revealed that the GC-EAD active region constituted (6Z,9Z)-heneicosadiene, (3Z,6Z,9Z)-heneicosatriene as active compounds. Preliminary wind tunnel studies for olfactory and behavioural responses showed blend of (6Z,9Z)-heneicosadiene (3 parts) + (3Z,6Z,9Z)-heneicosatriene (1 part) enticed 60% male adults. Interpretation: (6Z,9Z)-heneicosadiene and (3Z,6Z,9Z)-heneicosatriene are likely to be active pheromone components present in female sex pheromone glands. Blending of these two compounds in precise ratio can enhance the effectiveness of pheromone and can be used as effective strategy in jute IPM. Key words: Anomis sabulifera, Jute semilooper, Noctuidae, Sex pheromone, (6Z,9Z)-heneicosadiene, (3Z,6Z,9Z)-heneicosatriene

2018 ◽  
Vol 38 (04) ◽  
pp. 274-282
Author(s):  
K. R. M. Bhanu ◽  
D. R. Hall ◽  
R. V. Awalekar ◽  
M. Chandrashekharaiah ◽  
T. N. Divya ◽  
...  

AbstractThe leaf-eating caterpillar,Opisina arenosellaWalker, is the most destructive pest of coconut palm in India and Southeast Asia. The management practices employed againstO. arenosellaso far have been unsuccessful in many instances in India, due to the pest behaviour and coconut palm phenology. The life cycle, incidence and behaviour ofO. arenosellaare rather interesting and useful for the intervention of pheromone trapping technique for its management. We conducted the present study with the intention of identifying the female sex pheromone ofO. arenosellaand testing its efficacy under field conditions. Gas chromatography coupled electroantennographic detection (GC–EAD) and gas chromatography–mass spectrometry (GC–MS) analysis of female pheromone glands extract of one-day-oldO. arenosellafemales confirmed the presence of (Z,Z,Z)-3,6,9-tricosatriene (Z3Z6Z9-23Hy) as the dominant sex pheromone component. The male antennal response to female pheromone gland extract and syntheticZ3Z6Z9-23Hy was recorded using GC–EAD, and the results revealed that antennal response was positive to both the treatments at 0.13 mV and 0.14 mV respectively, compared to control (air), which was 0.016 mV. It was also evident from wind-tunnel experiments that the male moth response was high (80%) with the female gland extract, compared to 60% with synthetic pheromone and 0% for control (air). Male moths caught in the traps with and without lure were assessed in two field sites and recorded 69.26% and 54.25% more moth catches in the traps with the lure. We also observed a similar result in the cage experiment in which male moths caught in the traps with and without lure were 64.50% and 12.40%, respectively. The study also confirmed that 93.20% moths caught in the pheromone-baited traps were male. From the study, it is evident that the presence of (Z,Z,Z)-3,6,9-tricosatriene, which is the sex pheromone compound from the female gland extract ofO. arenosella, is an effective attractant in pheromone traps for the male moth under field conditions.


1980 ◽  
Vol 35 (1-2) ◽  
pp. 45-48 ◽  
Author(s):  
D. L. Struble ◽  
H. Arn ◽  
H. R. Buser ◽  
E. Städler ◽  
J. Freuler

Abstract Evidence obtained by glass capillary gas chromatography coupled to an electroantennographic detector or a mass spectrometer confirmed that Z-11-hexadecenyl acetate is the major component in the pheromone gland washes of calling Mamestra brassicae female moths. Three other components were identified, tetradecanyl acetate, hexadecanyl acetate and E-11-hexadecenyl acetate; but none of these had obvious synergistic effects in attracting males in field tests. The attraction of males to Z -11-hexadecenyl acetate was inhibited by 0.1% Z -11-hexadecenol or 1% Z-9- tetradecenyl acetate.


1997 ◽  
Vol 129 (6) ◽  
pp. 1001-1008 ◽  
Author(s):  
P.J. Silk ◽  
L.P.S. Kuenen ◽  
A.R. Alford ◽  
G.C. Lonergan ◽  
G.G. Grant

AbstractUsing gas chromatography and gas chromatography – mass spectrometry analyses of sex pheromone gland volatiles, we identified E- and Z-11-tetradecenals (E:Z-11-14:Aid) in an 85:15 blend of E:Z as the primary sex pheromone components of Croesia semipurpurana (Kearfott). Similar chemical analyses of sex pheromone gland extracts indicated the presence of the congeneric acetates (85:15 E:Z-11-14:Ac) and their saturated analogue (14:Ac); in addition, 85:15 E:Z-11-14:Alds were present at 5% of the acetates. The E:Z-11-14:Alds alone elicited trap capture; admixtures of saturated and unsaturated acetate analogues exhibited no significant synergistic or inhibitory effects. PVC rods with an 85:15 E:Z-11-14:Ald blend is recommended as a trap bait for monitoring population trends. It is unknown whether or not the components identified here represent the complete pheromone blend of this species; data from traps suspended 1.5 m above the ground versus those suspended 10 m above the ground suggest that future field studies may need to be conducted with traps positioned in the tree canopy.


2008 ◽  
Vol 61 ◽  
pp. 387-387
Author(s):  
V.J. Mitchell ◽  
L.M. Manning ◽  
A.M. El-Sayed

The New Zealand native moth Tmetolophota atristriga (Walker) (Lepidoptera Noctuidae) is a background pest defoliating pasture This project identified the pheromone components of the female pink grass worm that could be used to trap male moths in a control or monitoring programme Extraction of the sex pheromone gland of virgin female moths and GCMS analysis has identified several compounds in the sex pheromone gland two monounsaturated compounds cis11hexadecenal (Z1116Ald) and cis11hexadecyl acetate (Z1116Ac) and three saturated compounds hexadecan1ol (16OH) hexadecyl acetate (16Ac) and octadecan1ol (18OH) and triene hydrocarbon (ZZZ)369tricosatriene (Z3Z6Z923Hy) A field trapping experiment was conducted using binary blends of the two main compounds Z1116Ald and Z1116Ac at five different ratios (ie 1000 7525 5050 2575 and 0100) The highest catch was obtained at ratio 2575 of Z1116AldZ1116Ac; males were also caught at the 5050 ratio No catches were recorded with any other ratio tested A dose response experiment was conducted testing five loadings of the optimum binary (2575 ratio) mixture (01 1 10 100 and 1000 mg loading) and males were caught only at 01 and 1 mg loadings In a field trapping experiment conducted late in the season (2008) using the three additional minor compounds only the addition of Z3Z6Z923Hy to the binary mixture significantly enhanced male attraction


1984 ◽  
Vol 116 (5) ◽  
pp. 777-779 ◽  
Author(s):  
P. E. A. Teal ◽  
J. H. Tumlinson ◽  
J. R. McLaughlin ◽  
R. Heath ◽  
R. A. Rush

Fourteen and 16 carbon saturated and monounsaturated aldehydes have been identified as sex pheromone components for the four. species of Heliothis studied to date (Nesbitt et al. 1979; Klun et al. 1979; Teal et al. 1981). With the exception of H. zea (Boddie), the alcohol corresponding to the major aldehyde component, (Z)-1 l-hexadecen- 1-ol (Zl l-16:OH), also has been found in pheromone gland extracts of all species. We report here the identification of Zll-16:OH from pheromone gland extracts of H. zea females and the results of field trapping studies used to assess the behavioral effects of this compound on conspecific males.


1992 ◽  
Vol 18 (8) ◽  
pp. 1337-1347 ◽  
Author(s):  
Mikl�s T�th ◽  
Christer L�fstedt ◽  
Barry W. Blair ◽  
Tom�s Cabello ◽  
Ali I. Farag ◽  
...  

1987 ◽  
Vol 42 (11-12) ◽  
pp. 1347-1348 ◽  
Author(s):  
Zhu Pingchou ◽  
Kong Fanlei ◽  
Yu Shengdi ◽  
Yu Yongqing ◽  
Jin Shuping ◽  
...  

(E)11-Hexadecenyl acetate was identified from sex pheromone gland extract of female eggplant borer. The acetate synthesized in the laboratory showed high attractant activity in the field.


1978 ◽  
Vol 56 (2) ◽  
pp. 238-245 ◽  
Author(s):  
Jean Percy

In female Trichoplusia ni, granular haemocytes are observed near the basement membrane of developing sex pheromone gland cells while plasmatocytes are observed near the basement membrane of unmodified epidermal cells. The basement membrane underlying gland cells is clearly different from that of other epidermal cells. There is a thin amorphous layer (layer 1) which is also present beneath unmodified cells, and a second layer (layer 2) apposing the haemocytes. Layer 2 is distinctly banded which results from tubules similar in dimensions and structural appearance to those observed within granules of the granular haemocytes. The observations indicate that the granules participate in the formation of layer 2 by emptying their contents into the haemocoel next to layer 1.


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