scholarly journals Sex pheromone of the pink grass worm Tmetolophota atristriga (Walker)

2008 ◽  
Vol 61 ◽  
pp. 387-387
Author(s):  
V.J. Mitchell ◽  
L.M. Manning ◽  
A.M. El-Sayed

The New Zealand native moth Tmetolophota atristriga (Walker) (Lepidoptera Noctuidae) is a background pest defoliating pasture This project identified the pheromone components of the female pink grass worm that could be used to trap male moths in a control or monitoring programme Extraction of the sex pheromone gland of virgin female moths and GCMS analysis has identified several compounds in the sex pheromone gland two monounsaturated compounds cis11hexadecenal (Z1116Ald) and cis11hexadecyl acetate (Z1116Ac) and three saturated compounds hexadecan1ol (16OH) hexadecyl acetate (16Ac) and octadecan1ol (18OH) and triene hydrocarbon (ZZZ)369tricosatriene (Z3Z6Z923Hy) A field trapping experiment was conducted using binary blends of the two main compounds Z1116Ald and Z1116Ac at five different ratios (ie 1000 7525 5050 2575 and 0100) The highest catch was obtained at ratio 2575 of Z1116AldZ1116Ac; males were also caught at the 5050 ratio No catches were recorded with any other ratio tested A dose response experiment was conducted testing five loadings of the optimum binary (2575 ratio) mixture (01 1 10 100 and 1000 mg loading) and males were caught only at 01 and 1 mg loadings In a field trapping experiment conducted late in the season (2008) using the three additional minor compounds only the addition of Z3Z6Z923Hy to the binary mixture significantly enhanced male attraction

1984 ◽  
Vol 116 (5) ◽  
pp. 777-779 ◽  
Author(s):  
P. E. A. Teal ◽  
J. H. Tumlinson ◽  
J. R. McLaughlin ◽  
R. Heath ◽  
R. A. Rush

Fourteen and 16 carbon saturated and monounsaturated aldehydes have been identified as sex pheromone components for the four. species of Heliothis studied to date (Nesbitt et al. 1979; Klun et al. 1979; Teal et al. 1981). With the exception of H. zea (Boddie), the alcohol corresponding to the major aldehyde component, (Z)-1 l-hexadecen- 1-ol (Zl l-16:OH), also has been found in pheromone gland extracts of all species. We report here the identification of Zll-16:OH from pheromone gland extracts of H. zea females and the results of field trapping studies used to assess the behavioral effects of this compound on conspecific males.


1978 ◽  
Vol 56 (2) ◽  
pp. 238-245 ◽  
Author(s):  
Jean Percy

In female Trichoplusia ni, granular haemocytes are observed near the basement membrane of developing sex pheromone gland cells while plasmatocytes are observed near the basement membrane of unmodified epidermal cells. The basement membrane underlying gland cells is clearly different from that of other epidermal cells. There is a thin amorphous layer (layer 1) which is also present beneath unmodified cells, and a second layer (layer 2) apposing the haemocytes. Layer 2 is distinctly banded which results from tubules similar in dimensions and structural appearance to those observed within granules of the granular haemocytes. The observations indicate that the granules participate in the formation of layer 2 by emptying their contents into the haemocoel next to layer 1.


2006 ◽  
Vol 61 (3-4) ◽  
pp. 278-284 ◽  
Author(s):  
Raimondas Mozūraitis ◽  
Vidmantas Karaliusa ◽  
Vincas Būda ◽  
Anna-Karin Borg-Karlson

Gas chromatography and mass spectrometry analyses of crude sex pheromone gland extracts revealed that virgin Synanthedon tipuliformis (Clerck), currant borer (Lepidoptera: Sesiidae) females, produced 6 compounds, structurally related to sex pheromone components of clearwing moths. By comparison of retention times and mass spectra of natural products with corresponding properties of synthetic standards, these compounds were identified as: (2E,13Z)-octadeca-2,13-dien-1-yl acetate (E2,Z13-18:OAc), (3E,13Z)-octadeca-3,13-dien-1-yl acetate (E3,Z13-18:OAc), (13Z)-octadec-13-en-1-yl acetate (Z13-18:OAc), (2E,13Z)-octadeca- 2,13-dien-1-ol (E2,Z13-18:OH), (13Z)-octadec-13-en-1-ol (Z13-18:OH) and octadecan- 1-ol (18:OH) in the ratio 100:0.7:2.7:3.2:traces:traces. The first 3 compounds were previously known to occur in the sex pheromone gland extracts of currant borers, while the last 3 chemicals are now reported for the first time. Trapping tests carried out in the black currant field revealed that E2,Z13-18:OAc, when tested separately, attracted S. tipuliformis males, while addition of E3,Z13-18:OAc to the main component increased the effectiveness of E2,Z13-18:OAc over seven times. The attractiveness of 6 component lures did not differ significantly from the one of the binary mixture, confirming that E2,Z13-18:OAc and E3,Z13- 18:OAc in the ratio100:0.7 are essential sex pheromone components of S. tipuliformis. Trapping tests carried out at the dwelling place of Synanthedon scoliaeformis (Borkhausen) (Lepidoptera: Sesiidae) revealed that, in addition to intraspecific synergistic effect, E3,Z13-18:OAc increased the specificity of the pheromone signal of S. tipuliformis, acting by intraspecific mode as an attraction antagonist against S. scoliaeformis males. By this way, it ensured the specificity of the sex attraction signal of the currant borer. Consequently, both compounds E2,Z13-18:OAc and E3,Z13-18:OAc have to be present in pheromone formulations used for monitoring and/or control of S. tipuliformis to avoid effecting non-target species. Other compounds identified from the sex pheromone gland of S. tipuliformis did not show any significant interspecific activity for males of S. scoliaeformis, however, they provide a basis to achieve specificity of a pheromone signal of S. tipuliformis and could act as attraction antagonists against other clearwing moth species which, like S. tipuliformis, employ E2,Z13- 18:OAc as their sex pheromone component.


2012 ◽  
Vol 144 (6) ◽  
pp. 769-778 ◽  
Author(s):  
Gary J.R. Judd ◽  
Regine Gries ◽  
Carolyn Teasdale ◽  
Gerhard Gries

AbstractRaspberry crown borer, Pennisetia marginata (Harris) (Lepidoptera: Sesiidae), is a native North American species and pest of many cane fruits. Using coupled gas chromatographic–electroantennographic detection analyses (GC-EAD) we identified candidate sex pheromone components in pheromone gland extracts and effluvia from calling females. Analysis of gland extracts on a Zebron-5 column revealed four components (A, B, C, and D) that elicited strong responses from male antennae. The two most EAD-active components A and B were hypothesised to be (3E,13Z)-octadecadienal [(3E,13Z)-18:Ald] and (3E,13Z)-octadecadienol [(3E,13Z)-18:OH], respectively. Their retention times on other GC columns matched those of authentic standards, thus supporting structural assignments. Synthetic (3E,13Z)-18:Ald, and its GC rearrangement product (2E,13Z)-octadecadienal [(2E,13Z)-18:Ald; component C], also induced antennal responses in GC-EAD analyses of female effluvia. Compounds D in pheromone gland extract, and E and F in female effluvia, elicited EAD responses but could not be identified. In field trapping experiments, (3E,13Z)-18:Ald was the only component that attracted male P. marginata when tested alone. Attractiveness of (3E,13Z)-18:Ald was reduced when combined in binary or ternary blends with any of the other identified components, suggesting one or all may act as pheromone antagonists. In Aldergrove, British Columbia, Canada, peak diel attraction of male P. marginata to (3E,13Z)-18:Ald occurred between 16:00 and 18:00 hours Pacific Daylight Time, at temperatures of 21–23°C, on 30 August 2010, 3 September 2010, and 13 September 2010. We conclude that (3E,13Z)-18:Ald is the major pheromone component of P. marginata.


1997 ◽  
Vol 129 (6) ◽  
pp. 1001-1008 ◽  
Author(s):  
P.J. Silk ◽  
L.P.S. Kuenen ◽  
A.R. Alford ◽  
G.C. Lonergan ◽  
G.G. Grant

AbstractUsing gas chromatography and gas chromatography – mass spectrometry analyses of sex pheromone gland volatiles, we identified E- and Z-11-tetradecenals (E:Z-11-14:Aid) in an 85:15 blend of E:Z as the primary sex pheromone components of Croesia semipurpurana (Kearfott). Similar chemical analyses of sex pheromone gland extracts indicated the presence of the congeneric acetates (85:15 E:Z-11-14:Ac) and their saturated analogue (14:Ac); in addition, 85:15 E:Z-11-14:Alds were present at 5% of the acetates. The E:Z-11-14:Alds alone elicited trap capture; admixtures of saturated and unsaturated acetate analogues exhibited no significant synergistic or inhibitory effects. PVC rods with an 85:15 E:Z-11-14:Ald blend is recommended as a trap bait for monitoring population trends. It is unknown whether or not the components identified here represent the complete pheromone blend of this species; data from traps suspended 1.5 m above the ground versus those suspended 10 m above the ground suggest that future field studies may need to be conducted with traps positioned in the tree canopy.


2021 ◽  
Vol 42 (2) ◽  
pp. 254-264
Author(s):  
V.R. Babu ◽  
◽  
S. Satpathy ◽  
B.V.S. Reddy ◽  
◽  
...  

Aim: Identification and characterization of female released sex pheromone components of jute semilooper, Anomis sabulifera Guenee (Lepidoptera: Noctuidae) from female pheromone gland extracts. Methodology: Electroantennogram (EAG) was carried for studying the antennal response; Gas Chromatography coupled with Electro antenna Detector (GC-EAD) was conducted for studying the antennal response of eluted compounds from female pheromone gland extract; Gas Chromatography and Mas Spectrophotometry (GC-MS) was conducted for characterization or getting complete profile of compounds present in the female pheromone gland extract based on retention times. Wind tunnel assay was conducted for studying the behavioural responses of eluted compounds from the female pheromone gland extract. Results: GC-MS profile of female pheromone gland extract revealed that the GC-EAD active region constituted (6Z,9Z)-heneicosadiene, (3Z,6Z,9Z)-heneicosatriene as active compounds. Preliminary wind tunnel studies for olfactory and behavioural responses showed blend of (6Z,9Z)-heneicosadiene (3 parts) + (3Z,6Z,9Z)-heneicosatriene (1 part) enticed 60% male adults. Interpretation: (6Z,9Z)-heneicosadiene and (3Z,6Z,9Z)-heneicosatriene are likely to be active pheromone components present in female sex pheromone glands. Blending of these two compounds in precise ratio can enhance the effectiveness of pheromone and can be used as effective strategy in jute IPM. Key words: Anomis sabulifera, Jute semilooper, Noctuidae, Sex pheromone, (6Z,9Z)-heneicosadiene, (3Z,6Z,9Z)-heneicosatriene


2001 ◽  
Vol 56 (5-6) ◽  
pp. 423-429 ◽  
Author(s):  
Zhang Jintong ◽  
Han Yan ◽  
Meng Xianzuo

By means of thin-layer chromatography (TLC), electroantennogram (EAG), gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS) and field tests, (Z)-3-tetradecenyl acetate(Z3-14:Ac), (E)-3-tetradecen-1-ol(E3-14:OH), and (Z)-3-tetradecen-1-ol(Z3- 14:OH) at a ratio of 51:39:10 were identified from the female sex pheromone gland extracts of the carpenterworm, Holcocerus insularis Staudinger (Lepidoptera, Cossidae). The average amounts of Z3-14:Ac, E3-14:OH and Z3-14:OH in a single sex pheromone gland of calling moth were 7.29±2.72 ng, 5.72±2.43 ng and 1.44±0.56 ng, respectively. This is the first time that Z3-14:Ac was identified as a component of lepidopteran sex pheromone. Traps baited with rubber septa impregnated with Z3-14:Ac (500 μg / septum) were more effective than the traps baited with virgin female. The addition of the E3-14:OH and Z3-14:OH to rubber septa baited with Z3-14:Ac did not modify H. insularius male attraction, but E3-14:Ac slightly enhanced trap catch.


2002 ◽  
Vol 134 (4) ◽  
pp. 511-518 ◽  
Author(s):  
Y-X Liu ◽  
X-Z Meng

AbstractFour compounds were identified from female sex-pheromone gland extracts of Acleris fimbriana Merick, an important pest in northern fruit orchards of China, by gas chromatography, gas chromatography – mass spectrometry, and flight-tunnel and field-trapping tests. These compounds were (E)-11,13-tetradecadienal (E11,13-14:Ald), (E)-11,13-tetradecadien-1-ol (E11,13-14:OH), (E)-11-tetradecenyl acetate (E11-14:Ac), and (E)-11,13-tetradecadienyl acetate (E11,13-14:Ac). In field-trapping tests, traps baited with E11,13-14:Ald alone produced the highest trap captures of male A. fimbriana, but the other three individual compounds did not capture any mates. Addition of E11,13-14:Ac to E11,13-14:Ald enhanced trap capture, whereas addition of E11-14:Ac had no effect. A ternary blend of E11,13-14:Ald, E11,13-14:Ac, and E11-l4:Ac in a 6:4:1 ratio captured 7.5 times mean males per trap than virgin females. E11,13-14:OH inhibited trap capture when it was added to E11,13-14:Ald or the ternary blend. This pheromone could be used to monitor populations of A. fimbriana and to control this important pest of fruits in China.


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