Morphological and molecular characterization of Fusarium graminearum Schwabe as a causal agent of Hyssopus officinalis L. seed rot

Symptoms of seed rot of Hyssopus officinalis L. were noticed during seed health testing in 2018. According to morphological and cultural characteristics, isolates belong to Fusarium spp. and Alternaria spp.. Based on morphological and pathogenic properties, as well as sequence analysis, isolate designated as 4003/3 was identified as Fusarium graminearum deposited in NCBI gene bank under Acc. Number MK061542. To our knowledge F. graminearum as the causal agent of Hyssopus officinalis L. seed rot in Serbia was noticed for the first time.

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Morphological and molecular characterization of Fusarium spp. associated with yellowing disease of black pepper (Piper nigrum L.) in Malaysia

Journal of General Plant Pathology ◽

10.1007/s10327-012-0379-5 ◽

2012 ◽

Vol 78 (3) ◽

pp. 160-169 ◽

Cited By ~ 11

Author(s):

Sahar Shahnazi ◽

Sariah Meon ◽

Ganesan Vadamalai ◽

Khairulmazmi Ahmad ◽

Naghmeh Nejat

Keyword(s):

Molecular Characterization ◽

Black Pepper ◽

Piper Nigrum ◽

Fusarium Spp ◽

Yellowing Disease ◽

Morphological And Molecular Characterization

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Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil

Ciência Rural ◽

10.1590/0103-8478cr20141810 ◽

2015 ◽

Vol 45 (12) ◽

pp. 2197-2200 ◽

Cited By ~ 2

Author(s):

Thor Vinícius Martins Fajardo ◽

Monique Bezerra Nascimento ◽

Marcelo Eiras ◽

Osmar Nickel ◽

Gilvan Pio-Ribeiro

Keyword(s):

Amino Acid ◽

Molecular Characterization ◽

Molecular Analysis ◽

Nucleotide Sequences ◽

Amino Acid Sequences ◽

Causal Agent ◽

Ringspot Virus ◽

Prunus Necrotic Ringspot Virus ◽

First Time

ABSTRACT: There is no molecular characterization of Brazilian isolates of Prunus necrotic ringspot virus (PNRSV), except for those infecting peach. In this research, the causal agent of rose mosaic was determined and the movement (MP) and coat (CP) protein genes of a PNRSV isolate from rose were molecularly characterized for the first time in Brazil. The nucleotide and deduced amino acid sequences of MP and CP complete genes were aligned and compared with other isolates. Molecular analysis of the MP and CP nucleotide sequences of a Brazilian PNRSV isolate from rose and others from this same host showed highest identities of 96.7% and 98.6%, respectively, and Rose-Br isolate was classified in PV32 group.

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Morphological and Molecular Characterization of the Causal Agent of Downy Mildew on Quinoa (Chenopodium quinoa)

Mycopathologia ◽

10.1007/s11046-010-9272-y ◽

2010 ◽

Vol 169 (5) ◽

pp. 403-412 ◽

Cited By ~ 14

Author(s):

Young-Joon Choi ◽

Solveig Danielsen ◽

Mette Lübeck ◽

Seung-Beom Hong ◽

Rolf Delhey ◽

...

Keyword(s):

Downy Mildew ◽

Molecular Characterization ◽

Chenopodium Quinoa ◽

Causal Agent ◽

Morphological And Molecular Characterization

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Morphological and Molecular Characterization of Phoma complanata, a New Causal Agent of Archangelica officinalis Hoffm. in Poland

Polish Journal of Microbiology ◽

10.5604/01.3001.0010.7886 ◽

2017 ◽

Vol 66 (2) ◽

pp. 281-285 ◽

Cited By ~ 1

Author(s):

Beata Zimowska ◽

Ewa Dorota Zalewska ◽

Ewa Dorota Król ◽

Agnieszka Furmańczyk

Keyword(s):

Culture Media ◽

Culture Collection ◽

Morphological Similarity ◽

Reference Isolate ◽

Angelica Archangelica ◽

Its Regions ◽

Standard Culture ◽

First Time ◽

Morphological And Molecular Characterization

The paper concerns the fungus Phoma complanata, isolated for the first time in Poland, from the roots and umbels of angelica (Archangelica officinalis) in 2009. The morphology of fungal isolates was tested on standard culture media. Moreover, the sequence analysis of ITS regions was conducted. Morphological similarity of P. complanata Polish isolates to the reference isolate obtained from CBS culture collection was determined and together with the molecular analysis confirmed the affiliation of the fungus to the species.

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Morphological and Molecular Characterization of Entomophthorales (Entomophthoromycota: Entomophthoromycotina) from Argentina

Acta Mycologica ◽

10.5586/am.5522 ◽

2020 ◽

Vol 55 (2) ◽

Author(s):

Romina G. Manfrino ◽

Louela A. Castrillo ◽

Claudia C. López Lastra ◽

Andrea V. Toledo ◽

Walter Ferrari ◽

...

Keyword(s):

Molecular Data ◽

Molecular Techniques ◽

Accurate Identification ◽

Fungal Isolates ◽

Unidentified Species ◽

First Time ◽

Aphid Host ◽

Morphological And Molecular Characterization ◽

Molecular Characters

We characterized 17 insect-pathogenic entomophthoralean fungal isolates (Entomophthoromycotina: Entomophthorales) using morphological and molecular techniques. We identified four species from various insect hosts: (i) Entomophthora planchoniana, six specimens from aphids; (ii) Pandora neoaphidis, three specimens from aphids; (iii) Zoophthora phalloides from an aphid; and (iv) Z. radicans, seven specimens from insects in the orders Diptera, Hemiptera, and Lepidoptera. Analysis of ITS1 data from E. planchoniana showed clustering in accordance to aphid host species. Entomophthora planchoniana from Macrosiphum euphorbiae clustered together, separate from the isolate from Myzus persicae. The P. neoaphidis specimens clustered with sequences from other aphid-pathogenic Pandora species in GenBank. In this study, Z. phalloides from Brevicoryne brassicae and Z. radicans from an unidentified species of Chironomidae (Diptera) in Argentina were characterized for the first time. The present study was initiated to elucidate the taxonomy of the entomophthoralean fungi in Argentina according to their morphological and molecular characters. The presented results emphasize the significance of the combination of molecular data and information on morphology, ecology, and host range for accurate identification of entomophthoralean and allied genera.

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Morphological and molecular characterization of Fusarium spp. associated to the regional occurrence of wilt and dry bud rot in Agave tequilana

Revista Mexicana de Fitopatología Mexican Journal of Phytopathology ◽

10.18781/r.mex.fit.1911-4 ◽

2019 ◽

Vol 38 (1) ◽

Author(s):

Viridiana López-Bautista ◽

Gustavo Mora-Aguilera ◽

María Alejandra Gutiérrez-Espinosa ◽

Coral Mendoza-Ramos ◽

Verónica Inés Martínez-Bustamante ◽

...

Keyword(s):

Fusarium Oxysporum ◽

Molecular Characterization ◽

Fusarium Solani ◽

Fusarium Fujikuroi ◽

Agave Tequilana ◽

Fusarium Spp ◽

Impacto Económico ◽

Morphological And Molecular Characterization

La marchitez y pudrición seca del cogollo del agave (Agave tequilana var. azul) son enfermedades de alto impacto económico para este cultivo. En este trabajo se planteó determinar la implicación de Fusarium spp. en ambas enfermedades bajo un enfoque regional. Se colectaron muestras de raíz y suelo en 40 plantaciones comerciales ubicadas en 13 municipios de Los Altos Jalisco, importante región de cultivo de agave azul en México. De cada plantación de colecta se estimó carga de inóculo mediante un índice de Fusarium obtenido de unidades formadoras de colonias (Fusarium vs hongos totales) y se analizó su relación con pH y materia orgánica. Se obtuvieron 109 aislados caracterizados morfológicamente como Fusarium spp. de los cuales se seleccionaron 25 para identificación molecular con ITS y EF-1a. La selección consideró sintomatología, caracteres macro y microscópicos y prevalencia de tipologías de colonia observadas in vitro en medios Komada, Sabouraud, SNA y CLA. Los caracteres culturales y morfológicos evaluados fueron: coloración micelial, tamaño, forma y septación de macro y microconidios, y longitud y número de fiálides. Se asociaron cinco especies con marchitez y/o pudrición seca ubicadas en tres complejos filogenéticos: F. oxysporum del complejo de especies Fusarium oxysporum (FOSC) con 56% (46.2% suelo y 66.7% raíz) de representatividad regional; F. solani, F. falciforme y Fusarium sp. del complejo Fusarium solani (FSSC) (40%); y Fusarium sp. del complejo Fusarium fujikuroi (FFSC) (4%). MO y pH tuvieron correlación inversamente proporcional con Índice de Fusarium (r2 = 0.68-0.70). Se postula que la marchitez y pudrición seca del cogollo de agave azul constituyen un síndrome en el cual se asocian y especializan parasíticamente diversas especies de Fusarium. Se encontró un aislado de los tres complejos de Fusarium asociados específicamente a cada tipo de síntoma y la combinación de ellos. La mayoría se asociaron a marchitez con predominancia de F. oxysporum.

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Morphological and molecular characterization of pathogenic isolates of Fusarium spp. obtained from gladiolus corms and their sensitivity to Jatropha curcas L. oil

African Journal of Microbiology Research ◽

10.5897/ajmr2013.6413 ◽

2014 ◽

Vol 8 (8) ◽

pp. 724-733 ◽

Cited By ~ 3

Author(s):

Crdova Albores Liliana ◽

Bautista Baos Silvia ◽

Martnez Herrera Jorge ◽

Barrera Necha Laura ◽

Hernndez Lpez Mnica ◽

...

Keyword(s):

Molecular Characterization ◽

Jatropha Curcas ◽

Fusarium Spp ◽

Jatropha Curcas L ◽

Morphological And Molecular Characterization

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Pathogenicity and transmission of fungi detected on Passiflora alata seeds

Arquivos do Instituto Biológico ◽

10.1590/1808-1657000702017 ◽

2018 ◽

Vol 85 (0) ◽

Author(s):

João José Parisi ◽

Ivan Herman Fischer ◽

Priscila Fratin Medina ◽

Ana Carolina Firmino ◽

Laura Molina Meletti

Keyword(s):

Lower Cost ◽

Seedling Emergence ◽

Passion Fruit ◽

Seedling Production ◽

Fusarium Spp ◽

Disease Symptoms ◽

Fungal Isolates ◽

Seed Health ◽

Seed Rot ◽

Alternaria Sp

ABSTRACT: Passion fruit is usually propagated by seeds because of the ease and lower cost in seedling production. However, the seed is the most efficient agent for the spread of pathogens. The damages from seed-borne diseases occur mainly during the germination stages or at the formation of seedlings in nurseries. Considering the need for knowledge on the pathology of sweet passion fruit seeds, the objective was to evaluate the transmission and pathogenicity of the fungi Alternaria sp., Botrytis fabae, Cladosporium cladosporioides, Fusarium spp. and Lasiodiplodia theobromae, known as potentially pathogenic to this crop, and isolated from sweet passion fruit seeds. Therefore, tests on seed health, germination and seedling emergence in a sterilized commercial substrate were conducted using seeds from this species, inoculated with those fungal isolates. Leaves, stems and fruit from this plant were also inoculated with the same fungi. Alternaria sp., Fusarium spp. and L. theobromae were identified in seedlings obtained from inoculated seeds, confirming the transmission of these fungi by seeds. L. theobromae was also considered the most harmful fungus to passion fruit crop, as it causes seed rot and other disease symptoms on the leaves, stem and fruit. These findings inferred that healthy seeds of sweet passion fruit are essential for producing seedlings and to prevent the spread of the diseases caused by these fungi to exempt areas.

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