The importance of coconut seedling production in guyana

The demand for coconut water and coconut oil is increasing thus there is interest in expanding coconut cultivation. Coconut is primarily planted on approximately 28,500 acres by more than 1,400 farmers along the Guyana coastline with the Atlantic Ocean and in the Pomeroon Riverain area. Tall types (e.g. Jamaica Tall) are planted for oil while Dwarf types (e.g. Malaysian Dwarf Green, Suriname Brown) are planted primarily for water. At least 50% of the Tall type population is more than 50 years old and needs to be replanted to increase productivity. Farmers traditionally collect seedlings growing from dropped nuts at the base of trees without attention to prolificacy. Since a coconut tree productive life is more than 50 years, the economic impact of this practice can be significant. Via training programs, CARDI is encouraging the structured production of coconut seedlings to increase yields. This project aims to enhance the supply of quality seedlings using local seed nuts. Mother palms that display prolific bearing habits, resistance to pest and diseases plus vigorous growth habit were geotagged and recorded in a database. Seed nuts were purchased from farmers who own these trees and subsequently distributed (in batches of 400) to ten selected (age, gender, location) lead farmers to establish coconut seedling nurseries. Each lead farmer was assisted by 10 second ring farmers in the establishment of their nurseries under CARDI supervision. Results to date show that weed control and irrigation were important nursery activities. Some nurseries needed to be fenced to counter damage by animals (cows, sheep, goats). Average germination of seed nuts was approximately 50%. Available seedlings are being distributed to second ring farmers and lead farmers. CARDI will continue the national survey to identify outstanding mother palms. Measures to increase average germination must also be implemented. Local coconut seedling nurseries should therefore be encouraged as their “success” will alleviate the pressure to import seed nuts thus avoiding the attendant risk of introducing exotic diseases (e.g. lethal yellowing disease) into Guyana. Further, given that a coconut palm will live 50 to 70 years, the importance of carefully selecting premium quality coconut seedlings as a bedrock to develop and grow the industry cannot be over emphasized.

Modeling the Incidence of Yellowing Diseases to Whiteflies and Alternative Host

Yellowing diseases is one types of plant diseases which are found in some of the centers of cultivation of yard long bean in Bali. The spread of yellowing diseases caused the presence of vector insects, such as whiteflies and alternative host plants, such as weeds and other crops. This problem is then examined through statistical modeling in order to determine the pattern of the relationship between the incidence of yellowing disease with the populations of whiteflies and the alternative host plants. Collection of data on the yellowing disease incidences, the abundance of whiteflies, and the existence of alternative host plants was done through a direct observation in 100 farmer fields in several centers of cultivation of yard long beans in Bali. Determination of the best mathematical model was performed by linear regression analysis with ordinary least squares method. The results indicated that the best models for the relationship between the incidence of yellowing disease (KPK) with the population of whiteflies (KB) as well as the alternative host plants of yellowing were as follows: KPK = 1,2265 KB0,9872 TIAK1,0936 (R2 = 98,43 %; P-value = 0,000). The incidence of yellowing disease was highly significant positively correlated with the population of whiteflies and yellowing symptomatic alternative host plants. The existence of alternative host plants around the field was very decisive in triggering the emergence of yellow virus disease in the crop.

The importance of coconut seedling production in Guyana

The demand for coconut water and coconut oil is increasing thus there is interest in expanding coconut cultivation. Coconut is primarily planted on approximately 28,500 acres by more than 1,400 farmers along the Guyana coastline with the Atlantic Ocean and in the Pomeroon Riverain area. Tall types (e.g. Jamaica Tall) are planted for oil while Dwarf types (e.g. Malaysian Dwarf Green, Suriname Brown) are planted primarily for water. At least 50% of the Tall type population is more than 50 years old and needs to be replanted to increase productivity. Farmers traditionally collect seedlings growing from dropped nuts at the base of trees without attention to prolificacy. Since a coconut tree productive life is more than 50 years, the economic impact of this practice can be significant. Via training programs, CARDI is encouraging the structured production of coconut seedlings to increase yields. This project aims to enhance the supply of quality seedlings using local seed nuts. Mother palms that display prolific bearing habits, resistance to pest and diseases plus vigorous growth habit were geotagged and recorded in a database. Seed nuts were purchased from farmers who own these trees and subsequently distributed (in batches of 400) to ten selected (age, gender, location) lead farmers to establish coconut seedling nurseries. Each lead farmer was assisted by 10 second ring farmers in the establishment of their nurseries under CARDI supervision. Results to date show that weed control and irrigation were important nursery activities. Some nurseries needed to be fenced to counter damage by animals (cows, sheep, goats). Average germination of seed nuts was approximately 50%. Available seedlings are being distributed to second ring farmers and lead farmers. CARDI will continue the national survey to identify outstanding mother palms. Measures to increase average germination must also be implemented. Local coconut seedling nurseries should therefore be encouraged as their “success” will alleviate the pressure to import seed nuts thus avoiding the attendant risk of introducing exotic diseases (e.g. lethal yellowing disease) into Guyana. Further, given that a coconut palm will live 50 to 70 years, the importance of carefully selecting premium quality coconut seedlings as a bedrock to develop and grow the industry cannot be over emphasized.

Oil Palm Fatal Yellowing (FY), a Disease with an Elusive Causal Agent

Fatal yellowing disease (FY) is a bud rot-type disease that severely affects oil palm plantations in Latin America. Since 1974, when it was first reported in Brazil, this disorder has been responsible for severe economic losses in the oil palm industry; and, for nearly 50 years, several studies have tried to identify its causal agent, without success. The etiological studies regarding FY in oil palm explored either biotic and abiotic stress scenarios, in a single or combined manner. Most recently, the hypothesis in favor of one biotic cause has lost some grounds to the abiotic one, mainly due to new insights regarding deficient aeration in the soil, which reduces the potential for oxy-reduction, causing changes in the ionic composition of the soil solution. This review presents an overview of the history of this disease and the several efforts done to fulfill Koch’s postulates over the last 40 years, besides discussing recent studies that revisited this subject using some omics technics. We conclude by discussing further uses of omics via a multi-omics integration (MOI) strategy to help finally find out what is really behind the genesis of FY. Finding this elusive causal agent of FY out will allow either the development of a more efficient diagnostic tool and the advance in studies trying to find out the source of the genetic resistance hidden in the genome of the American oil palm.

Genome characterization of 'Candidatus Phytoplasma meliae' (isolate ChTYXIII)

Candidatus Phytoplasma meliae (subgroups 16SrXIII-G and XIII-C) has been reported in association to chinaberry yellowing disease in Argentina, Bolivia and Paraguay. In Argentina, this disease constitutes a major phytosanitary problem for chinaberry forestry production. To date, no genome information of this phytoplasma and others from 16SrXIII-group has been published, hindered its characterization at genomic level. Here we analyze the draft genome of Candidatus Phytoplasma meliae strain ChTYXIII obtained from a chinaberry-infected plant using a metagenomics approach. The draft assembly consists of twenty-one contigs with a total length of 751.949 bp. The annotation contains 669 CDSs, 34tRNA and one set of rRNA operons. Metabolic pathways analysis indicated that the ChTYXIII contains the complete core genes for glycolysis and functional sec system for translocation of proteins. The phylogenetic relationships inferred 132 single copy genes (orthologues core) analysis revealed that Ca. P. meliae constitutes a clade closely related to the Ca. australiense and Ca. P. solani. Thirty-one putative effectors were identified, among which a homologue to SAP11 was found and others that have only been described in this pathogen. This work provides relevant genomic information for Ca. P. meliae and constitutes the first genome described for the group 16SrXIII (MPV).