Интродукция трех видов пряно-ароматических растений семейства Lamiaceae Lindl.

Представлены результаты многолетнего изучения особенностей сезонного ритма роста и развития, морфометрические параметры и успешность интродукции 3 представителей семейства Lamiaceae Lindl. (Яснотковые): Dracocephalum nutans L. (змееголовник поникающий), Hyssopus officinalis L. (иссоп лекарственный), Lavandula angustifolia Mill. (лаванда узколистная). Исследования выполнены на коллекционной экспозиции «Ароматный сад» в условиях Башкирского Предуралья. Изученные вышеуказанные виды растений благодаря наличию биологически активных веществ, эфирных масел обладают лекарственными и пищевыми свойствами, также эти виды отличаются высокой декоративностью. За вегетационный период, который в этих условиях начинается со 2-й декады апреля и продолжается в среднем до середины октября, исследованные таксоны проходят полный жизненный цикл, включая образование плодов и созревание семян. Они успешно прошли интродукционные испытания в условиях Башкирского Предуралья, являются перспективными для выращивания в качестве декоративных растений в миксбордерах, ароматических садах и в качестве пряного и лекарственного сырья.

Antioxidant defense and secondary metabolites concentration in hyssop (Hyssopus officinalis L.) plants as affected by salt stress

Salt stress is one of the major limiting factors for plant production, and the quality of medicinal plants is also affected by soil salinity. Hyssop (Hyssopus officinalis L.) plants were cultivated for four weeks in perlite: sand and irrigated with Hoagland nutrient solution containing 0, 50, 100, 150, and 200 mM NaCl. Plants growth was decreased by salt stress while the leaf relative water content was not affected, and the chlorophyll content decreased only by the highest salt concentration (200 mM). Sodium was accumulated at small amounts, indicating a high ability of this species to exclude salt. Soluble sugars and proline were accumulated up to 1.6 and 4.5 fold, respectively. The antioxidant enzymes activity (peroxidase, catalase, ascorbate peroxidase) were increased by the salt treatments, particularly in the leaves. The levels of secondary metabolites (saponins, phenolics, flavonoids, anthocyanins, and iridoids) were all increased under salt stress, and the total antioxidant capacity of alcoholic extract of the leaves and roots was significantly higher in the salt-treated compared with control plants. Our results showed that hyssop is a salt-tolerant species, and the quality of this medicinal plant is improved when grown under saline conditions.

ANTI-INFLUENZA ACTIVITY OF PLANT EXTRACTS OF THE LAMIACEAE FAMILY

In this work, it was revealed that water and ethanol extracts of the plants Nepeta cataria, Nepeta sibirica, Scutellaria baicalensis, Hyssopus officinalis, Betonica officinalis, and water extracts of Dracocephalum moldavica, Glechoma hederacea, Mentha arvensis, Prunella vulgaris, Melissa officinalis ethanol extracts of Mentha piperita, Mentha crispa, Origanum vulgare, Hyssopus officinalis, Salvia verticillata showed antiviral activity against the influenza virus subtype H5N1. Aqueous extracts of Nepeta cataria and Glechoma hederacea (NI 3.75) showed the highest antiviral effect against the H5N1 subtype. It was revealed the antiviral activity against influenza virus subtype H3N2 of aqueous and ethanol extracts of Scutellaria baicalensis, Mentha piperita, Mentha arvensis, Mentha crispa, aqueous extract of Dracocephalum moldavica and ethanol extracts of Glechoma hederacea, Origanum vulgare, Prunella vulgaris, Hyssopus officinalis, Betonica officinalis, Salvia verticillata. Ethanolic extract of Betonica officinalis (NI 4.25) showed the highest virus neutralizing activity against the H3N2 subtype. It was carried out chemical analysis of the aerial parts of plants of the Lamiaceae family. It was shown that the highest content of flavonols was observed in Dracocephalum nutans (4.47±0.04%), the highest content of tannins was found in Mentha arvensis (17.62 ± 0.78%), and the highest content catechins were found in Nepeta cataria (0.43 ± 0.007%). Thus, plant extracts of the Lamiaceae family are promising sources for further studies to develop new antiviral drugs.

Evaluation of the Conditions for the Cultivation of Callus Cultures of Hyssopus officinalis Regarding the Yield of Polyphenolic Compounds

The cultivation of plants in the form of callus cultures constitutes a renewable source of secondary plant metabolites. The conditions for the cultivation of callus cultures affect the yield of target compounds. Callus cultures of Hyssopus officinalis were chosen for study. Nutrient media of various compositions were used for Hyssopus officinalis callus culture. For each culture, data on the quantitative contents of saponins, flavonoids and polyphenolic compounds, as well as antioxidant activity, were obtained. It was found that Murashige and Skoog medium supplemented with 1-naphthylacetic acid and kinetin led to the highest yield of secondary metabolites.

Phytotoxic Effect of Essential Oil from Hyssop (Hyssopus officinalis L.) against Spring Wheat and White Mustard

Hyssop essential oil is a rich source of biologically active compounds. This study aimed to characterize the chemical composition of essential oil from the hyssop herb and its phytotoxicity against germination and initial growth of wheat and mustard. The main compounds of the oil were identified as isopinocamphone and pinocamphone. In a Petri dish experiment, the oil mainly inhibited the germination and initial growth of wheat, whereas mustard was less affected. In conclusion, hyssop oil displays phytotoxic potential against the studied species and should be tested further.

Antioxidant, Antigenotoxic and Cytotoxic Activity of Essential Oils and Methanol Extracts of Hyssopus officinalis L. Subsp. aristatus (Godr.) Nyman (Lamiaceae)

Hyssopus officinalis L. is a well-known aromatic plant used in traditional medicine and the food and cosmetics industry. The aim of this study is to assess the antioxidant, genotoxic, antigenotoxic and cytotoxic properties of characterized hyssop essential oils and methanol extracts. Chemical composition was analyzed by gas chromatography - mass spectrometry (GC-MS) and liquid chromatography with diode array detection and mass spectrometry (LC-DAD-MS), respectively. Antioxidant activity was examined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) tests; genotoxic and antigenotoxic activity were examined by the comet assay, while cytotoxicity was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye (MTT) test against tumor cell lines (SW480, MDA-MB 231, HeLa) and non-transformed human lung fibroblast cell lines (MRC-5). The essential oils were rich in monoterpene hydrocarbons (e.g., limonene; 7.99–23.81%), oxygenated monoterpenes (1,8-cineole; 38.19–67.1%) and phenylpropanoids (methyl eugenol; 0.00–28.33%). In methanol extracts, the most abundant phenolics were chlorogenic and rosmarinic acid (23.35–33.46 and 3.53–17.98 mg/g, respectively). Methanol extracts expressed moderate to weak antioxidant activity (DPPH IC50 = 56.04–199.89 µg/mL, FRAP = 0.667–0.959 mmol Fe2+/g). Hyssop preparations significantly reduced DNA damage in human whole blood cells, induced by pretreatment with hydrogen peroxide. Methanol extracts exhibited selective and potent dose- and time-dependent activity against the HeLa cell line. Results of the current study demonstrated notable H. officinalis medicinal potential, which calls for further investigation.