Psilotum nudum: Bárrios, S. & Copeland, A.

Author(s):  
Keyword(s):  
Author(s):  
P. Dayanandan ◽  
P. B. Kaufman

A three dimensional appreciation of the guard cell morphology coupled with ultrastjuctural studies should lead to a better understanding of their still obscure dynamics of movement. We have found the SEM of great value not only in studies of the surface details of stomata but also in resolving the structures and relationships that exist between the guard and subsidiary cells. We now report the isolation and SEM studies of guard cells from nine genera of plants.Guard cells were isolated from the following plants: Psilotum nudum, four species of Equisetum, Cycas revoluta, Ceratozamia sp., Pinus sylvestris, Ephedra cochuma, Welwitschia mirabilis, Euphorbia tirucalli and Allium cepa.


2012 ◽  
Vol 128 (3-4) ◽  
pp. 95-99 ◽  
Author(s):  
James E. Mickle ◽  
Maria Rosaria Barone Lumaga ◽  
Paolo De Luca

Abstract Apical regions of developing aerial shoots of Psilotum nudum (L.) Beauv. were studied using both scanning electron microscopy (SEM) and light microscopy (LM) with the aim of improving our understanding of early stages in stomatal and epidermal ontogenesis. SEM samples were fixed in gluteraldehyde, critical point dried, and coated with an Au-Pd alloy. LM samples were fixed in FAA and embedded in paraffin. LM sections were stained with 0.05% toluidine blue for protein. SEM shows that P. nudum stomata develop from 20 µm-long domed meristemoid cells into guard cell mother cells (GMCs). A furrow dividing guard cells develops at 30 µm long, and wax deposition that will cover the entire cell begins at 70 µm long. LM longitudinal sections of GMCs show a cytoplasmic protein net that organizes into radial fibers, similar to reports of actin fibers in stomata of angiosperms. This study provides additional details of stomatal development in Psilotum and is the first report of an actin-like protein net in Psilotum.


1987 ◽  
Vol 74 (9) ◽  
pp. 1438-1445 ◽  
Author(s):  
Paul J. Schulte ◽  
Arthur C. Gibson ◽  
Park S. Nobel

1971 ◽  
Vol 61 (3) ◽  
pp. 141
Author(s):  
Fredrick C. Boutin
Keyword(s):  

Chromosoma ◽  
1988 ◽  
Vol 97 (1) ◽  
pp. 96-100 ◽  
Author(s):  
Lorinda K. Anderson ◽  
Stephen M. Stack

Planta ◽  
2001 ◽  
Vol 214 (1) ◽  
pp. 75-84 ◽  
Author(s):  
Yasuyo Yamazaki ◽  
Dae-Yeon Suh ◽  
Worapan Sitthithaworn ◽  
Kazuhiko Ishiguro ◽  
Yukie Kobayashi ◽  
...  

1995 ◽  
Vol 85 (3) ◽  
pp. 95
Author(s):  
James H. Peck ◽  
James R. Bray ◽  
Daniel L. Marsh ◽  
Dennis W. McMasters ◽  
Carl Amason ◽  
...  
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1981 ◽  
Vol 59 (5) ◽  
pp. 711-720 ◽  
Author(s):  
R. L. Peterson ◽  
Melanie J. Howarth ◽  
Dean P. Whittier

Mature Psilotum gametophytes found in greenhouse pots containing plants of Hoya, Philodendron, Aspidistra, or Diffenbachia were processed for microscopy. An endophytic fungus was abundant in the rhizoids and in most cortical parenchyma cells except at the growing apices. Although the fungus has not been identified, it is an aseptate fungus with coarse hyphae which occasionally form vesicles. Endophytic fungal hyphae store quantities of lipid which appear to be released into the host cytoplasm upon fungal degeneration. This lipid and the remnants of hyphal cell walls may be used as an energy source by the achlorophyllous gametophyte. Gametophyte cell organelles, including the nucleus, appear to degenerate after fungal breakdown, and the cells presumably die. Although reinfection of cells containing degenerated hyphae was found, it was not particularly common.


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