Agarose Droplet Method of Macrophage Migration-Inhibition Test for Newcastle Disease Virus in Chickens

1976 ◽  
Vol 20 (2) ◽  
pp. 230 ◽  
Author(s):  
C. Morita ◽  
T. Okada ◽  
H. Izawa ◽  
M. Soekawa
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Inhibition Test ◽  
Macrophage Migration ◽  
Migration Inhibition ◽  
Macrophage Migration Inhibition ◽  
Droplet Method

Is injectable collagen truly safe?

1992 ◽  
Vol 106 (8) ◽  
pp. 704-708 ◽  
Author(s):  
Etsuyo Takayama ◽  
Makoto Ikeda ◽  
Sumiaki Tsuru ◽  
Masami Ogura ◽  
Satoshi Kitahara ◽  
...  
Keyword(s):  
Allergic Reaction ◽  
Positive Response ◽  
Inhibition Test ◽  
Macrophage Migration ◽  
Potential Hazard ◽  
Migration Inhibition ◽  
Injectable Collagen ◽  
Macrophage Migration Inhibition

AbstractMost patients have no response to injectable collagen or silicone, but some cases may have positive or ‘undersea’(= clinically negative but immunologically positive) response to collagen. From the results of the Macrophage migration inhibition test, the relative immunogenicity was augmented most when we used implants with the following combination. The firstimmunization was collagen and the second one was collagen with silicone.The augmented antigenicity might be enough to cause an allergic reaction to the patients who had no response to each implant alone. The purpose of this article is to warn of the potential hazard of injection.

Titration of Newcastle disease virus and its neutralizing antibodies in microplates by a modified hemadsorption and hemadsorption inhibition method

1976 ◽  
Vol 3 (3) ◽  
pp. 227-232
Author(s):  
Israel Institute for Biological Research, Ness-Ziona, Israel
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Chicken Embryo ◽  
Neutralizing Antibodies ◽  
Inhibition Test ◽  
Virus Infectivity ◽  
Embryo Fibroblasts ◽  
Microneutralization Test ◽  
Chicken Embryo Fibroblasts

Using the microtiter system, titration of Newcastle disease virus infectivity and neutralizing antibodies was carried out in chicken embryo fibroblasts grown in "U" or flat-bottomed plates. Infectivity was detected by a combined hemadsorption-hemagglutination method. Inhibition of that reaction indicated the presence of neutralizing antibodies. A 24-h microneutralization test was developed and compared to the plaque neutralization and microhemagglutination inhibition test. Reproducibility of the microneutralization test was statistically analyzed.

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