Effects of Intraspecific Variation on Phylogenetic Inference: A Likelihood Analysis of mtDNA Restriction Site Data in Cyprinid Fishes

1991 ◽  
Vol 40 (4) ◽  
pp. 393 ◽  
Author(s):  
Peter E. Smouse ◽  
Thomas E. Dowling ◽  
Joseph A. Tworek ◽  
Walter R. Hoeh ◽  
Wesley M. Brown
1991 ◽  
Vol 40 (4) ◽  
pp. 393-409
Author(s):  
P. E. Smouse ◽  
T. E. Dowling ◽  
J. A. Tworek ◽  
W. R. Hoeh ◽  
W. M. Brown

Zootaxa ◽  
2020 ◽  
Vol 4858 (1) ◽  
pp. 1-34
Author(s):  
THAMARA ZACCA ◽  
MIRNA M. CASAGRANDE ◽  
OLAF H. H. MIELKE ◽  
BLANCA HUERTAS ◽  
MARIANNE ESPELAND ◽  
...  

Vareuptychia Forster, 1964 stat. rest. is revalidated and comprises two species, V. similis (Butler, 1867) comb. rest. and V. themis (Butler, 1867) comb. nov. Vanima Zacca, Casagrande & Mielke gen. nov. is described to contain Euptychia labe Butler, 1870 (the type species), E. palladia Butler, 1867 and E. lesbia Staudinger, [1886]. The taxonomy of these two genera was initially revised based on morphological and distributional data, and subsequently tested and supported with a Maximum Likelihood analysis using four genes (COI, GAPDH, RpS5 and EF1-a). Lectotypes are designated for Euptychia similis Butler, 1867, E. themis Butler, 1867, E. undina Butler, 1870 and E. lesbia Staudinger, [1886]. No DNA sequences were obtained for Euptychia cleophes Godman & Salvin, 1889 but its transfer to Megisto Hübner, [1819] is supported by morphological evidence. For all taxa treated in this study, a taxonomic catalog, diagnosis, (re)description and illustrations of adults, venation and genitalia are provided, as well as comments on intraspecific variation, sexual dichromatism, ecology and distribution maps. 


Copeia ◽  
1973 ◽  
Vol 1973 (1) ◽  
pp. 45 ◽  
Author(s):  
Joseph T. Eastman ◽  
James C. Underhill

Author(s):  
Ruiyan Luo ◽  
Andrew L Hipp ◽  
Bret Larget

Amplified Fragment Length Polymorphism (AFLP) markers are formed by selective amplification of DNA fragments from digested total genomic DNA. The technique is popular because it is a relatively inexpensive way to produce large numbers of reproducible genetic markers. In this paper, we describe a Bayesian approach to modeling AFLP marker evolution by nucleotide substitution and an MCMC approach to estimate phylogeny from AFLP marker data. We demonstrate the method on species in Carex section Ovales, a group of sedges common in North America. We compare the results of our analysis with a clustering method based on Nei and Li's restriction-site distance and a two-state Bayesian analysis using MrBayes.


1981 ◽  
Vol 81 (2) ◽  
pp. 429-451 ◽  
Author(s):  
R. B. Cattell ◽  
D. C. Rao ◽  
L.R. Schmidt ◽  
D. S. Vaughan

2011 ◽  
pp. 28-35
Author(s):  

Background: The C677T polymorphism of MTHFR gene is a risk factor of many diseases. This study is aimed at: (1) Improving a PCR-RFLP process with the own designed primers to identify the C677T polymorphism of MTHFR gene. (2) Evaluating the prevalence of the C677T polymorphism of MTHFR gene in volunteer group. Materials and method: DNA samples was extracted from peripheral blood of 60 volunteers. Designing primers by using FastPCR software, then improving PCR technique. Standardizing the optimal conditions of restriction digest by HinfI. Confirming the results of polymorphism by DNA sequencing technique. Results: We designed successfully primers to amplify fragment of MTHFR gene including C677T polymorphism and an obligatory restriction site of HinfI (as internal control). 0.5 µl of HinfI enzyme (10 U/µl) is enough for restriction digest. The MTHFR genotype frequencies were: 71.67 % (677CC); 25% (677CT); and 3.33 % (677TT). Conclusion: We standardized successfully PCR-RFLP technique to identifying C677T polymorphism of MTHFR gene. Keywords: C677T polymorphism, MTHFR gene, PCR-RFLP


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