Blood Changes in Calcium and Magnesium Ion Concentrations after Whole-Body X-Irradiation

1970 ◽  
Vol 42 (3) ◽  
pp. 560 ◽  
Author(s):  
L. Ababei ◽  
C. Haler ◽  
M. Trandafirescu ◽  
D. Cernătescu ◽  
M. Ionescu ◽  
...  
Keyword(s):  
Whole Body ◽  
Magnesium Ion ◽  
Ion Concentrations ◽  
X Irradiation ◽  
Calcium And Magnesium

STUDY OF SOME FACTORS THAT AFFECT THE DISTRIBUTION OF NUCLEAR VOLUMES IN PREPARATIONS OF RAT LIVER NUCLEI

1967 ◽  
Vol 45 (7) ◽  
pp. 1175-1183 ◽  
Author(s):  
Roberto Umaña
Keyword(s):  
Rat Liver ◽  
Dna Content ◽  
Direct Relationship ◽  
Magnesium Ion ◽  
Homogenization Procedure ◽  
Ion Concentrations ◽  
Ph Values ◽  
Sucrose Solutions ◽  
Liver Nuclei ◽  
Calcium And Magnesium

The effect of the homogenization procedure, the centrifugation scheme, and the composition of the suspension medium on the distribution of nuclear volumes has been studied.It has been shown that the Waring Blendor not only destroys a greater number of the nuclei during homogenization, but also that this destruction is a selective one. At neutral pH values, no direct relationship appears to exist between the DNA content of the nuclei and their density. For this reason, purification in concentrated sucrose solutions produces a selective loss of the lighter nuclei, which includes small diploid stromal nuclei and some of the larger polyploid type of parenchymal nuclei.The study of the effect of increasing the calcium and magnesium ion concentrations (from 0.001 to 0.005 M) on the nuclear distribution showed that these ions produce a selective shrinkage and condensation of the nuclei, probably through different mechanisms.

scholarly journals Changes in Sodium, Calcium, and Magnesium Ion Concentrations That Inhibit Geobacillus Biofilms Have No Effect on Anoxybacillus flavithermus Biofilms

2015 ◽  
Vol 81 (15) ◽  
pp. 5115-5122 ◽  
Author(s):  
B. Somerton ◽  
D. Lindsay ◽  
J. Palmer ◽  
J. Brooks ◽  
S. Flint
Keyword(s):  
Biofilm Formation ◽  
Magnesium Ion ◽  
Content Type ◽  
High Sodium ◽  
Ion Concentrations ◽  
Sodium Calcium ◽  
Viable Cells ◽  
Anoxybacillus Flavithermus ◽  
Calcium And Magnesium ◽  
The Difference

ABSTRACTThis study investigated the effects of varied sodium, calcium, and magnesium concentrations in specialty milk formulations on biofilm formation byGeobacillusspp. andAnoxybacillus flavithermus. The numbers of attached viable cells (log CFU per square centimeter) after 6 to 18 h of biofilm formation by three dairy-derived strains ofGeobacillusand three dairy-derived strains ofA. flavithermuswere compared in two commercial milk formulations. Milk formulation B had relatively high sodium and low calcium and magnesium concentrations compared with those of milk formulation A, but the two formulations had comparable fat, protein, and lactose concentrations. Biofilm formation by the threeGeobacillusisolates was up to 4 log CFU cm−2lower in milk formulation B than in milk formulation A after 6 to 18 h, and the difference was often significant (P≤ 0.05). However, no significant differences (P≤ 0.05) were found when biofilm formations by the threeA. flavithermusisolates were compared in milk formulations A and B. Supplementation of milk formulation A with 100 mM NaCl significantly decreased (P≤ 0.05)Geobacillusbiofilm formation after 6 to 10 h. Furthermore, supplementation of milk formulation B with 2 mM CaCl2or 2 mM MgCl2significantly increased (P≤ 0.05)Geobacillusbiofilm formation after 10 to 18 h. It was concluded that relatively high free Na+and low free Ca2+and Mg2+concentrations in milk formulations are collectively required to inhibit biofilm formation byGeobacillusspp., whereas biofilm formation byA. flavithermusis not impacted by typical cation concentration differences of milk formulations.

scholarly journals INTRACELLULAR LOCALIZATION OF ENZYMES IN SPLEEN

1962 ◽  
Vol 12 (2) ◽  
pp. 263-276 ◽  
Author(s):  
Herbert J. Eichel ◽  
Jay S. Roth
Keyword(s):  
Substrate Concentration ◽  
Intracellular Localization ◽  
Maximal Activity ◽  
Mitochondrial Fraction ◽  
Whole Body ◽  
Magnesium Ion ◽  
Supernatant Fluid ◽  
Ribonuclease Inhibitor ◽  
X Irradiation ◽  
Triton X

Some properties of rat spleen ribonuclease have been studied, and the intracellular distribution of the enzyme and ribonucleic acid have been presented. Spleen ribonuclease exhibits maximal activity at pH 5.8, and although there is some evidence for the presence of an enzyme with an optimum at pH 7.0, it is not conclusive. The enzyme is concentrated primarily in the mitochondrial fraction, but significant quantities occur in the supernatant fluid. The latter contains ribonuclease inhibitor similar to that found in liver. The effects of whole body x-irradiation, magnesium ion, substrate concentration, type of buffer, presence of p-chloromercuriphenylsulfonic acid, deoxycholate, and Triton X-100 on ribonuclease activity are examined.

scholarly journals Response in Growth, Scute Development, and Whole-Body Ion Composition of Acipenser fulvescens Reared in Water of Differing Chemistries

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1419
Author(s):  
Janet Genz ◽  
Rachael Hicks
Keyword(s):  
River Water ◽  
Growth And Development ◽  
Lake Sturgeon ◽  
Whole Body ◽  
Acipenser Fulvescens ◽  
Water Type ◽  
Ion Composition ◽  
Ion Concentrations ◽  
Before And After ◽  
Yolk Absorption

In fishes, environmental ion availability can have substantial effects on growth and development. This study examined the development of Lake Sturgeon in response to the varying environmental ion availability that they experience as part of a conservation stocking program. We reared sturgeon in natural water from the Coosa River, which had higher concentrations of Mg2+, Na+, and Zn2+ than standard hatchery conditions, while [Ca2+] at the Warm Springs National Fish Hatchery was 2× higher than in the Coosa River. Eggs were hatched in each water type and the larvae were sampled at time points before and after yolk absorption during the first 8 weeks of development. Total length and weight in WSNFH larvae were significantly higher than larvae in Coosa River water starting at 8 dph, indicating that growth was dependent on the different environmental ion levels. Concentrations of the ions of interest were also determined for whole-body acid digests of the exposed Lake Sturgeon. We found that Lake Sturgeon reared in Coosa River water had significantly higher magnesium and zinc than Lake Sturgeon reared in WSNFH water (p < 0.05), while calcium was significantly higher in WSNFH than Coosa River water. This difference shows that different environmental ion concentrations also impact the overall development of larval Lake Sturgeon.

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