Molecular Phylogenetics of Ramaria and Related Genera: Evidence from Nuclear Large Subunit and Mitochondrial Small Subunit rDNA Sequences

Mycologia ◽  
2001 ◽  
Vol 93 (3) ◽  
pp. 465 ◽  
Author(s):  
Andrea J. Humpert ◽  
Eric L. Muench ◽  
Admir J. Giachini ◽  
Michael A. Castellano ◽  
Joseph W. Spatafora
Keyword(s):  
Molecular Phylogenetics ◽  
Large Subunit ◽  
Small Subunit ◽  
Rdna Sequences ◽  
Small Subunit Rdna

Molecular phylogenetics of Ramaria and related genera: evidence from nuclear large subunit and mitochondrial small subunit rDNA sequences

Mycologia ◽  
2001 ◽  
Vol 93 (3) ◽  
pp. 465-477 ◽  
Author(s):  
Andrea J. Humpert ◽  
Eric L. Muench ◽  
Admir J. Giachini ◽  
Michael A. Castellano ◽  
Joseph W. Spatafora
Keyword(s):  
Molecular Phylogenetics ◽  
Large Subunit ◽  
Small Subunit ◽  
Rdna Sequences ◽  
Small Subunit Rdna

On the phylogeny of Ophiostoma, Ceratocystis s.s., and Microascus, and relationships within Ophiostoma based on partial ribosomal DNA sequences

1993 ◽  
Vol 71 (9) ◽  
pp. 1249-1265 ◽  
Author(s):  
G. Hausner ◽  
J. Reid ◽  
G. R. Klassen
Keyword(s):  
Dna Sequences ◽  
Sequence Data ◽  
Large Subunit ◽  
Small Subunit ◽  
Data Set ◽  
Rdna Sequences ◽  
Morphological Criteria ◽  
Small Subunit Rdna ◽  
Rdna Sequence Data ◽  
Large Subunit Rdna

Phylogenetic analysis of partial rDNA sequences suggests that Ophiostoma should remain the sole genus of the Ophiostomataceae, and this should be the sole family within the Ophiostomatales, whereas Ceratocystis s.s. would be best disposed within the Microascales. Although morphological criteria suggest that the genus Ophiostoma is heterogeneous, analysis of partial small subunit rDNA sequence data shows that Ophiostoma (excluding O. roraimense) represents a monophyletic taxon. Analysis of a partial large subunit rDNA data set, which included sequences from 55 species assignable to Ophiostoma, failed to support the strict subdivision of the genus based on either ascospore characters or the nature of the anamorph. Key words: Ceratocystis, Microascus, Ophiostoma, partial rDNA sequences, phylogeny.

scholarly journals A new subclade of haplontic Metschnikowia species associated with insects of morning glory flowers in Africa and description of Metschnikowia aberdeeniae sp. nov.

2006 ◽  
Vol 56 (5) ◽  
pp. 1141-1145 ◽  
Author(s):  
Marc-André Lachance ◽  
T. Michael Anderson ◽  
William T. Starmer
Keyword(s):  
National Park ◽  
Large Subunit ◽  
Small Subunit ◽  
Morning Glory ◽  
The Novel ◽  
Rdna Its ◽  
Rdna Sequences ◽  
Small Subunit Rdna ◽  
Exact Position ◽  
Is Strain

The novel species Metschnikowia aberdeeniae is described to accommodate five isolates recovered from insects of morning glory flowers in the Serengeti National Park, Tanzania. On the basis of rDNA ITS and D1/D2 large-subunit sequences, these yeasts form, together with six other isolates, a novel subclade of large-spored Metschnikowia species. The exact position of the subclade within the Metschnikowiaceae cannot be determined with any confidence from these sequences or from small-subunit rDNA sequences, as the variable sites of the sequences are excessively divergent. However, in morphological and physiological terms, the novel isolates are typical of the genus Metschnikowia in general and of the large-spored group in particular. The type strain of Metschnikowia aberdeeniae sp. nov. is strain SUB 05-213.1T (=CBS 10289T=NRRL Y-27921T) (h−) and the allotype is strain SUB 05-213.2 (=CBS 10290=NRRL Y-27922) (h+).

Evolution of RuminantSarcocystis(Sporozoa) Parasites Based on Small Subunit rDNA Sequences

1999 ◽  
Vol 11 (1) ◽  
pp. 27-37 ◽  
Author(s):  
O.J.M. Holmdahl ◽  
David A. Morrison ◽  
John T. Ellis ◽  
Lam T.T. Huong
Keyword(s):  
Small Subunit ◽  
Rdna Sequences ◽  
Small Subunit Rdna

Discovery of a free-living nematode phylogenetically related to vertebrate parasites of the genus Strongyloides (Nematoda : Strongyloidoidea): morphological, anatomical and molecular characterisation

2016 ◽  
Vol 30 (4) ◽  
pp. 387
Author(s):  
Ren-E Huang ◽  
Runsheng Li ◽  
Zhongying Zhao
Keyword(s):  
Culture Media ◽  
Large Subunit ◽  
Molecular Genetic ◽  
Small Subunit ◽  
Land Snails ◽  
Rrna Genes ◽  
Lsu Rdna ◽  
Free Living ◽  
Rdna Sequences ◽  
Free Living Nematode

Nematodes are important, largely because they frequently act as parasites and threaten the health of plants, animals and even humans. Here, we describe an interesting free-living nematode from land snails on Luofu Mountain, Guangdong, China. Alloionema luofuensis, sp. nov. is phylogenetically related to slug-parasite A. appendiculatum and the well-known vertebrate parasites Strongyloides spp. based on small subunit (SSU) and the D2-D3 domain of large subunit (LSU) rDNA sequences. The new species possesses an extremely transparent body and is easily maintained using C. elegans culture media, suggesting a possible application prospect of this free-living nematode as a comparative model system for its related parasites. Morphology and anatomy of the gonochoristic A. luofuensis, sp. nov. adult were described and illustrated. The species is characterised by a filiform tail bisexually, ‘rhabditiform’ oesophagus and ‘rhabditid-like’ female anatomy, but its male caudal region is completely different from that of typical rhabditid nematodes, being absent from an enveloping bursa. It is the first marker taxon characterised morphologically as well as molecularly from the family Alloionematidae, a group of nematodes with hyperdiverse molecular genetic variations underlying highly conserved anatomy. Further molecular and genetic studies on A. luofuensis, sp. nov. populations hold promise to provide insight into evolution of the clade consisting of vertebrate parasites of the heterogonic nematode genus Strongyloides. This is because of its unusual high levels of heterozygosity maintained by the conserved rRNA genes of partial SSU and the D2-D3 domain of LSU for the type isolate of this species.

scholarly journals The endemic Cladophorales (Ulvophyceae) of ancient Lake Baikal represent a monophyletic group of very closely related but morphologically diverse species

2020 ◽  
Author(s):  
C Boedeker ◽  
F Leliaert ◽  
OA Timoshkin ◽  
VS Vishnyakov ◽  
S Díaz-Martínez ◽  
...  
Keyword(s):  
Lake Baikal ◽  
Phylogenetic Signal ◽  
Small Subunit ◽  
Monophyletic Group ◽  
Species Flock ◽  
Surprising Result ◽  
Internal Transcribed Spacer Region ◽  
Rdna Sequences ◽  
Green Algal ◽  
Small Subunit Rdna

© 2018 Phycological Society of America Lake Baikal, the oldest lake in the world, is home to spectacular biodiversity and extraordinary levels of endemism. While many of the animal species flocks from Lake Baikal are famous examples of evolutionary radiations, the lake also includes a wide diversity of endemic algae that are not well investigated with regards to molecular-biological taxonomy and phylogeny. The endemic taxa of the green algal order Cladophorales show a range of divergent morphologies that led to their classification in four genera in two families. We sequenced partial large- and small-subunit rDNA as well as the internal transcribed spacer region of 14 of the 16 described endemic taxa to clarify their phylogenetic relationships. One endemic morphospecies, Cladophora kusnetzowii, was shown to be conspecific with the widespread Aegagropila linnaei. All other endemic morphospecies formed a monophyletic group nested within the genus Rhizoclonium (Cladophoraceae), a very surprising result, in stark contrast to their morphological affinities. The Baikal clade represents a species flock of closely related taxa with very low genetic differentiation. Some of the morphospecies were congruent with lineages recovered in the phylogenies, but due to the low phylogenetic signal in the rDNA sequences the relationships within the Baikal clade were not all well resolved. The Baikal clade appears to represent a recent radiation, based on the low molecular divergence within the group, and it is hypothesized that the large morphological variation results from diversification in sympatry from a common ancestor in Lake Baikal.

scholarly journals Isolation and Characterization of a Novel Facultative Anaerobic Filamentous Fungus from Japanese Rice Field Soil

2009 ◽  
Vol 2009 ◽  
pp. 1-9 ◽  
Author(s):  
Akio Tonouchi
Keyword(s):  
Filamentous Fungus ◽  
Rice Field ◽  
Large Subunit ◽  
Small Subunit ◽  
Lsu Rdna ◽  
Field Soil ◽  
Rice Field Soil ◽  
Rdna Sequences ◽  
Isolation And Characterization

A novel filamentous fungus strain designated RB-1 was isolated into pure culture from Japanese rice field soil through an anaerobic role tube technique. The strain is a mitosporic fungus that grows in both aerobic and strict anaerobic conditions using various mono-, di-, tri-, and polysaccharides with acetate and ethanol productions. The amount of acetate produced was higher than that of ethanol in both aerobic and anaerobic cultures. The characteristic verrucose or punctuate conidia of RB-1 closely resembled those of some strains of the genusThermomyces, a thermophilic or mesophilic anamorphic ascomycete. However, based on phylogenetic analysis with the small subunit (SSU) and large subunit (LSU) rDNA sequences, RB-1 was characterized as a member of the class Lecanoromycetes of the phylum Ascomycota. Currently, RB-1 is designated as an anamorphic ascomycete and is phylogenetically considered anincertae sediswithin the class Lecanoromycetes.

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