Factors Influencing Zoospore Production in Liquid Cultures of Lagenidium giganteum (Oomycetes, Lagenidiales)

Mycologia ◽  
1982 ◽  
Vol 74 (5) ◽  
pp. 820 ◽  
Author(s):  
A. J. Domnas ◽  
S. M. Fagan ◽  
S. Jaronski

Mycologia ◽  
1982 ◽  
Vol 74 (5) ◽  
pp. 820-825 ◽  
Author(s):  
A. J. Domnas ◽  
S. M. Fagan ◽  
S. Jaronski


1980 ◽  
Vol 58 (19) ◽  
pp. 2117-2122 ◽  
Author(s):  
Calvin L. Schoulties ◽  
Kenneth F. Baker ◽  
Carol Sabersky-Lehmann

Factors and procedures found to increase the quantity and consistency of axenic zoospore production in a selected isolate of Phytophthora cinnamomi were (i) the use of single-zoospore cultures of uniform size that were between 48 and 72 h old; (ii) thorough washing of mycelial mats at the time of sporangium induction to remove nutrients; (iii) agitation of the sporulation medium (mineral salt solution) 24 h after the initial induction; (iv) standardization of the volume of the sporulation medium; (v) adequate removal of the sporulation medium and replacement with distilled water before triggering zoospore release; and (vi) placement of colonies that had been induced to sporulate under light. The addition of a purified sporangium stimulatory substance to mycelial mats which had been induced to sporulate enabled the fungus to sporulate under conditions which normally suppressed sporulation in vitro. In the presence of this stimulatory substance, the fungus sporulated prolifically in darkness and with limited quantities of added nutrients. Other isolates of P. cinnamomi responded in a similar manner to many of these factors and procedures.



Author(s):  
Julie A. Martini ◽  
Robert H. Doremus

Tracy and Doremus have demonstrated chemical bonding between bone and hydroxylapatite with transmission electron microscopy. Now researchers ponder how to improve upon this bond in turn improving the life expectancy and biocompatibility of implantable orthopedic devices.This report focuses on a study of the- chemical influences on the interfacial integrity and strength. Pure hydroxylapatite (HAP), magnesium doped HAP, strontium doped HAP, bioglass and medical grade titanium cylinders were implanted into the tibial cortices of New Zealand white rabbits. After 12 weeks, the implants were retrieved for a scanning electron microscopy study coupled with energy dispersive spectroscopy.Following sacrifice and careful retrieval, the samples were dehydrated through a graduated series starting with 50% ethanol and continuing through 60, 70, 80, 90, 95, and 100% ethanol over a period of two days. The samples were embedded in LR White. Again a graduated series was used with solutions of 50, 75 and 100% LR White diluted in ethanol.



1965 ◽  
Vol 8 (4) ◽  
pp. 323-347
Author(s):  
Robert Goldstein ◽  
Benjamin RosenblÜt

Electrodermal and electroencephalic responsivity to sound and to light was studied in 96 normal-hearing adults in three separate sessions. The subjects were subdivided into equal groups of white men, white women, colored men, and colored women. A 1 000 cps pure tone was the conditioned stimulus in two sessions and white light was used in a third session. Heat was the unconditioned stimulus in all sessions. Previously, an inverse relation had been found in white men between the prominence of alpha rhythm in the EEG and the ease with which electrodermal responses could be elicited. This relation did not hold true for white women. The main purpose of the present study was to answer the following questions: (1) are the previous findings on white subjects applicable to colored subjects? (2) are subjects who are most (or least) responsive electrophysiologically on one day equally responsive (or unresponsive) on another day? and (3) are subjects who are most (or least) responsive to sound equally responsive (or unresponsive) to light? In general, each question was answered affirmatively. Other factors influencing responsivity were also studied.



1950 ◽  
Vol 16 (1) ◽  
pp. 194-210 ◽  
Author(s):  
Frederick W. Hoffbauer ◽  
Jesse L. Bollman ◽  
John L. Grindlay




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