Histological changes in periodontal tissues of rat molars following perforation of the pulp and its floor.

Objective. To investigate oxidative stress (OS) and histological changes that occur in the periodontium of subjects with type 2 diabetes mellitus without signs of periodontal disease and to establish if oxidative stress is a possible link between diabetes mellitus and periodontal changes.Materials and Methods. Tissue samples from ten adult patients with type 2 diabetes mellitus (T2D) and eight healthy adults were harvested. The specimens were examined by microscope using standard hematoxylin-eosin stain, at various magnifications, and investigated for tissue levels of malondialdehyde (MDA) and glutathione (GSH).Results. Our results showed that periodontal tissues in patients with T2D present significant inflammation, affecting both epithelial and connective tissues. Mean MDA tissue levels were 3.578 ± 0.60 SD in diabetics versus 0.406 ± 0.27 SD in controls (P< 0.0001), while mean GSH tissue levels were 2.48 ± 1.02 SD in diabetics versus 9.7875 ± 2.42 SD in controls (P< 0.0001).Conclusion. Diabetic subjects had higher MDA levels in their periodontal tissues, suggesting an increased lipid peroxidation in T2D, and decreased GSH tissue levels, suggesting an alteration of the local antioxidant defense mechanism. These results are in concordance with the histological changes that we found in periodontal tissues of diabetic subjects, confirming the hypothesis of OS implication, as a correlation between periodontal disease incidence and T2D.

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Chronic Laser Exposure Effects on Rabbit Retina

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100093523 ◽

1972 ◽

Vol 30 ◽

pp. 54-55

Author(s):

Burton B. Silver ◽

Theodore Lawwill

Keyword(s):

Laser Irradiation ◽

Laser Light ◽

Broad Band ◽

Argon Laser ◽

Atropine Sulfate ◽

Ultrastructural Changes ◽

Laser Exposure ◽

Rabbit Retina ◽

Histological Changes ◽

Threshold Doses

Dutch-belted 1 to 2.5 kg anesthetized rabbits were exposed to either xenon or argon laser light administered in a broad band, designed to cover large areas of the retina. For laser exposure, the pupil was dilated with atropine sulfate 1% and pheny lephrine 10%. All of the laser generated power was within a band centered at 5145.0 Anstroms. Established threshold for 4 hour exposures to laser irradiation are in the order of 25-35 microwatts/cm2. Animals examined for ultrastructural changes received 4 hour threshold doses. These animals exhibited ERG, opthalmascopic, and histological changes consistent with threshold damage.One month following exposure the rabbits were killed with pentobarbitol. The eyes were immediately enucleated and dissected while bathed in 3% phosphate buffered gluteraldehyde.

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The influence of margins of restorations on the periodontal tissues over 26 years

Journal Of Clinical Periodontology ◽

10.1034/j.1600-051x.2001.280109.x ◽

2001 ◽

Vol 28 (1) ◽

pp. 57-64 ◽

Cited By ~ 65

Author(s):

Marc Schatzle ◽

Niklaus P. Lang ◽

Age Anerud ◽

Hans Boysen ◽

Walter Burgin ◽

...

Keyword(s):

Periodontal Tissues

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Early histological changes in the aortic media after thoracic endovascular stent grafting (TEVAR)

The Thoracic and Cardiovascular Surgeon ◽

10.1055/s-0031-1297723 ◽

2012 ◽

Vol 60 (S 01) ◽

Author(s):

J Scheumann ◽

MS Bischoff ◽

C Heilmann ◽

M Siepe ◽

F Roder ◽

...

Keyword(s):

Endovascular Stent ◽

Histological Changes ◽

Stent Grafting ◽

Endovascular Stent Grafting ◽

Aortic Media

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A Computerized Coding System for Processing Basic Histopathological Changes – Application to Vascular Pathology

Methods of Information in Medicine ◽

10.1055/s-0038-1635460 ◽

1986 ◽

Vol 25 (03) ◽

pp. 139-142 ◽

Cited By ~ 2

Author(s):

A. Mauriello ◽

Y. Sambuy ◽

E. Bonanno ◽

A. Orlandi ◽

G. Palmieri ◽

...

Keyword(s):

Vascular Wall ◽

Final Diagnosis ◽

Vascular Pathology ◽

Histopathological Study ◽

Coding System ◽

Histopathological Changes ◽

Histological Changes ◽

Pathological Conditions ◽

Computer Based ◽

Sufficient Space

SummaryAmong the numerous existing computer-based systems for processing pathological data, none contains sufficient space for encoding data on the basic cytological or histological changes of a certain organ or tissue, upon which the final diagnosis is based.An “analytical record” was constructed listing all the basic changes that can be encountered in the various pathological conditions of the vascular wall. The data collected on the “analytical record” were coded by means of an alphanumeric code and stored in an Apple II 48 K minicomputer.The advantages of this system include the computerization of the data by non-specialized personnel and the possibility to’ quantitatively analyze the histocytopathological parameters used for diagnosis in vascular pathology. This coding system may easily be adapted, with minor modifications, to the histopathological study of other organs and tissues.

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