Detection of Micrometastases in Sentinel Lymph Nodes of Melanoma Patients by RT-PCR.

2002 ◽  
Vol 64 (2) ◽  
pp. 211-217 ◽  
Author(s):  
Yoichi MOROI ◽  
Chikage OBATA ◽  
Shohei FUJITA ◽  
Satoko SHIBATA ◽  
Sawako KONISHI ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Sentinel Lymph Nodes ◽  
Rt Pcr ◽  
Melanoma Patients

Prognostic value of tyrosinase RT-PCR analysis in histologically negative sentinel lymph nodes from melanoma patients

2004 ◽  
Vol 22 (14_suppl) ◽  
pp. 7562-7562
Author(s):  
M. Gonzalez Cao ◽  
C. Badenas ◽  
J. Malvehy ◽  
R. Martí ◽  
T. Castel ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Prognostic Value ◽  
Sentinel Lymph Nodes ◽  
Pcr Analysis ◽  
Rt Pcr ◽  
Melanoma Patients

Molecular Staging of Pathologically Negative Sentinel Lymph Nodes from Melanoma Patients Using Multimarker, Quantitative Real-Time RT-PCR

2009 ◽  
Vol 16 (1) ◽  
Author(s):  
Josep M. Hilari ◽  
Cristina Mangas ◽  
Liqiang Xi ◽  
Cristina Paradelo ◽  
Carlos Ferrándiz ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Real Time ◽  
Sentinel Lymph Nodes ◽  
Rt Pcr ◽  
Molecular Staging ◽  
Melanoma Patients

Survivin, bcl-2, bax, and bcl-X Gene Expression in Sentinel Lymph Nodes From Melanoma Patients

2003 ◽  
Vol 21 (2) ◽  
pp. 306-312 ◽  
Author(s):  
Angela Gradilone ◽  
Paola Gazzaniga ◽  
Diego Ribuffo ◽  
Susanna Scarpa ◽  
Emanuele Cigna ◽  
...  
Keyword(s):  
Gene Expression ◽  
Lymph Nodes ◽  
Survivin Expression ◽  
Sentinel Lymph Nodes ◽  
Rt Pcr ◽  
Survivin Gene ◽  
Variable Expression ◽  
Melanoma Patients ◽  
X Gene ◽  
Disease Free

Purpose: The expression of apoptosis-related genes, such as survivin, bcl-2, bcl-X, and bax, has been evaluated by reverse transcriptase polymerase chain reaction (RT-PCR) and by immunohistochemistry in sentinel lymph nodes (SLNs) from melanoma patients and then correlated to the outcome of patients. Patients and Methods: Thirty-six SLNs were examined. After RNA extraction, an RT-PCR followed by Southern blot hybridization was performed to detect survivin, bcl-2, bcl-X, and bax mRNA. bcl-2, survivin, and bax gene expression was evaluated, whenever possible, also by immunohistochemistry at the protein level. Results: We found a significant correlation (P < .005) between survivin expression and outcome of patients; in fact, 61.5% of patients expressing survivin gene progressed or died because of the disease, whereas 38.5% are currently disease-free. Among patients negative for survivin expression, 100% are disease-free after a median follow-up time of 52.9 months. We did not find a significant correlation between bcl-2, bax, and bcl-X gene expression and outcome of patients. In fact, these genes were found equally expressed in patients with disease progression and in disease-free patients. Conclusion: Our findings show a variable expression of apoptosis-related genes in SLNs of melanoma patients; more interestingly, we found that survivin expression correlates to outcome of patients in a statistically significant way, whereas the expression of other genes, such as bcl-2, bax, and bcl-X, did not seem to correlate to progression of disease. We suggest that the detection of survivin gene expression by RT-PCR in SLNs may be a useful prognostic indicator.

Quantitative real-time RT-PCR in sentinel lymph nodes from melanoma patients.

Apmis ◽  
2008 ◽  
Vol 116 (3) ◽  
pp. 199-205 ◽  
Author(s):  
RIKKE RIBER-HANSEN ◽  
HELENE NORTVIG ABRAHAMSEN ◽  
BOE SANDAHL SORENSEN ◽  
STEPHEN JACQUES HAMILTON-DUTOIT ◽  
TORBEN STEINICHE
Keyword(s):  
Lymph Nodes ◽  
Real Time ◽  
Sentinel Lymph Nodes ◽  
Rt Pcr ◽  
Melanoma Patients

Prognostic value of tyrosinase RT-PCR analysis in histologically negative sentinel lymph nodes from melanoma patients

2004 ◽  
Vol 22 (14_suppl) ◽  
pp. 7562-7562
Author(s):  
M. Gonzalez Cao ◽  
C. Badenas ◽  
J. Malvehy ◽  
R. Martí ◽  
T. Castel ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Prognostic Value ◽  
Sentinel Lymph Nodes ◽  
Pcr Analysis ◽  
Rt Pcr ◽  
Melanoma Patients

scholarly journals Prognostic Significance of Molecular Upstaging of Paraffin-Embedded Sentinel Lymph Nodes in Melanoma Patients

2004 ◽  
Vol 22 (13) ◽  
pp. 2671-2680 ◽  
Author(s):  
Hiroya Takeuchi ◽  
Donald L. Morton ◽  
Christine Kuo ◽  
Roderick R. Turner ◽  
David Elashoff ◽  
...  
Keyword(s):  
Overall Survival ◽  
Prognostic Factor ◽  
Lymph Nodes ◽  
Risk Ratio ◽  
Disease Recurrence ◽  
Sentinel Lymph Nodes ◽  
Independent Prognostic Factor ◽  
Number Of Patients ◽  
Significant Independent Prognostic Factor ◽  
Melanoma Patients

PurposeDetection of micrometastases in sentinel lymph nodes (SLNs) is important for accurate staging and prognosis in melanoma patients. However, a significant number of patients with histopathology-negative SLNs subsequently develop recurrent disease. We hypothesized that a quantitative realtime reverse transcriptase polymerase chain reaction (qRT) assay using multiple specific mRNA markers could detect occult metastasis in paraffin-embedded (PE) SLNs to upstage and predict disease outcome.Patients and MethodsqRT was performed on retrospectively collected PE SLNs from 215 clinically node-negative patients who underwent lymphatic mapping and sentinel lymphadenectomy for melanoma and were followed up for at least 8 years. PE SLNs (n = 308) from these patients were sectioned and assessed by qRT for mRNA of four melanoma-associated genes: MART-1 (antigen recognized by T cells-1), MAGE-A3 (melanoma antigen gene-A3 family), GalNAc-T (β1→4-N-acetylgalactosaminyl-transferase), and Pax3 (paired-box homeotic gene transcription factor 3).ResultsFifty-three (25%) patients had histopathology-positive SLNs by hemotoxylin and eosin and/or immunohistochemistry. Of the 162 patients with histopathology-negative SLNs, 48 (30%) had nodes that expressed at least one of the four qRT markers, and these 48 patients also had a significantly increased risk of disease recurrence by a Cox proportional hazards model analysis (P < .0001; risk ratio, 7.48; 95% CI, 3.70 to 15.15). The presence of ≥ one marker in histopathology-negative SLNs was also a significant independent prognostic factor by multivariate analysis for overall survival (P = .0002; risk ratio, 11.42; 95% CI, 3.17 to 41.1).ConclusionMolecular upstaging of PE histopathology-negative SLNs by multiple-marker qRT assay is a significant independent prognostic factor for long-term disease recurrence and overall survival of patients with early-stage melanoma.

Clinical importance and surgical management of sentinel lymph nodes in the popliteal fossa of melanoma patients

2019 ◽  
Vol 45 (9) ◽  
pp. 1706-1711 ◽  
Author(s):  
Amanda A.G. Nijhuis ◽  
Ivan D. de A.O. Santos Filho ◽  
Roger F. Uren ◽  
John F. Thompson ◽  
Omgo E. Nieweg
Keyword(s):  
Lymph Nodes ◽  
Surgical Management ◽  
Clinical Importance ◽  
Sentinel Lymph Nodes ◽  
Popliteal Fossa ◽  
Melanoma Patients
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