1919-P: Dysfunction of BCAA Catabolism in Pancreatic Alpha Cells Is Associated with Higher Glucagon Secretion in Diabetic Mice

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 1919-P
Author(s):  
ERI WADA ◽  
MASAKI KOBAYASHI ◽  
OSAMU KIKUCHI ◽  
DAISUKE KOHNO ◽  
TADAHIRO KITAMURA
2018 ◽  
Vol 6 (17) ◽  
pp. e13852 ◽  
Author(s):  
Reshma Ramracheya ◽  
Caroline Chapman ◽  
Margarita Chibalina ◽  
Haiqiang Dou ◽  
Caroline Miranda ◽  
...  

1998 ◽  
Vol 76 ◽  
pp. 100
Author(s):  
Hiroo Kawajiri ◽  
Na Qiao ◽  
Da-Ming Zhuang ◽  
Tanihiro Yoshimoto ◽  
Miyuki Yamamoto ◽  
...  

2015 ◽  
Vol 36 (5) ◽  
pp. 1735-1742 ◽  
Author(s):  
Ling Chen ◽  
Jing Zhang ◽  
Zhuo Zhang ◽  
Yaping Chu ◽  
Bing Song ◽  
...  

Background/Aims: The lack of available beta cells greatly limits the use of beta cell transplantation as a therapy for diabetes. Thus, generation of beta cells from other sources is substantially required. Pax4 has been shown to induce reprograming of alpha cells into beta cells during embryogenesis. Nevertheless, whether expression of Pax4 in adult alpha cells could trigger this alpha-to-beta cell reprogramming is unknown. Methods: Here we generated an adeno-associated virus carrying Pax4 and GFP under a CMV promoter (AAV-Pax4). We used AAV-Pax4 to transduce a mouse alpha cell line in vitro, and to transduce primary alpha cells in diabetic mice. Reprogramming was examined by double immunostaining and by changes in beta cell number. The effects on blood glucose were evaluated by fasting blood glucose and glucose response. Results: In vitro, Pax4 overexpression neither induced insulin expression, nor suppressed glucagon expression in alpha cells. In vivo, Pax4 overexpression failed to increase beta cell number, and did not alter hyperglycemia and glucose response in diabetic mice. Conclusion: Pax4 expression is not sufficient to transduce pancreatic alpha cells into beta cells. Overexpression of Pax4 in alpha cells may not increase functional beta cell number in diabetic patients.


2015 ◽  
Vol 4 (4) ◽  
pp. 277-286 ◽  
Author(s):  
Gao Sun ◽  
Gabriela da Silva Xavier ◽  
Tracy Gorman ◽  
Claire Priest ◽  
Antonia Solomou ◽  
...  

2016 ◽  
Vol 110 (3) ◽  
pp. 452a
Author(s):  
Morten G. Pedersen ◽  
Ingela Ahlstedt ◽  
Eva-Marie Andersson ◽  
Sven Göpel

Diabetologia ◽  
1995 ◽  
Vol 38 (4) ◽  
pp. 422-429 ◽  
Author(s):  
K. Kisanuki ◽  
H. Kishikawa ◽  
E. Araki ◽  
T. Shirotani ◽  
M. Uehara ◽  
...  

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