scholarly journals A Common Hormone-Sensitive Lipase i6 Gene Polymorphism Is Associated With Decreased Human Adipocyte Lipolytic Function

Diabetes ◽  
2001 ◽  
Vol 50 (10) ◽  
pp. 2410-2413 ◽  
Author(s):  
J. Hoffstedt ◽  
P. Arner ◽  
M. Schalling ◽  
N. L. Pedersen ◽  
S. Sengul ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Hormone Sensitive Lipase ◽  
Human Adipocyte ◽  
Hormone Sensitive

The hormone-sensitive lipase i6 gene polymorphism and body fat accumulation

2002 ◽  
Vol 32 (12) ◽  
pp. 938-942 ◽  
Author(s):  
C. Lavebratt ◽  
M. Rydén ◽  
M. Schalling ◽  
S. Sengul ◽  
S. Ahlberg ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Body Fat ◽  
Hormone Sensitive Lipase ◽  
Fat Accumulation ◽  
Hormone Sensitive

scholarly journals Characterization of the promoter of human adipocyte hormone-sensitive lipase

1997 ◽  
Vol 328 (2) ◽  
pp. 453-461 ◽  
Author(s):  
Jacques GROBER ◽  
Henrik LAURELL ◽  
Régis BLAISE ◽  
Béatrice FABRY ◽  
Stéphane SCHAAK ◽  
...  
Keyword(s):  
Genomic Sequence ◽  
Transcriptional Start Site ◽  
Regulatory Elements ◽  
Rnase H ◽  
Hormone Sensitive Lipase ◽  
Start Codon ◽  
Start Site ◽  
Rnase Protection ◽  
Human Adipocyte ◽  
Hormone Sensitive

Hormone-sensitive lipase (HSL) catalyses the rate-limiting step of adipose tissue lipolysis. The human HSL gene is composed of nine exons encoding the adipocyte form and a testis-specific coding exon. Northern blot analyses showed that human adipocytes express a 2.8 kb HSL mRNA, suggesting the presence of a short (20-150 bp) 5ʹ untranslated region (5ʹ-UTR). A single 5ʹ-UTR of approx. 70 nt was detected in RNase H mapping experiments. Two 5ʹ-UTRs of 70 and 170 nt respectively were obtained by rapid amplification of cDNA ends and cDNA library screenings. RNase protection experiments, with probes derived from the two products, showed that human adipocyte HSL mRNA contains only the 70 nt product. Primer extension analysis mapped the transcriptional start site 74 nt upstream of the start codon. In HT29, a human cell line expressing HSL, the presence of the short or the long 5ʹ-UTR is mutually exclusive. The short and long 5ʹ-UTR exons were located 1.5 and approx. 13 kb respectively upstream of the first coding exon. Various portions of the 5ʹ-flanking region upstream of the short product exon were linked to the luciferase gene and transfected into cells that express HSL (HT29 cells and rat adipocytes) and do not express HSL (HeLa cells). High luciferase activity was found for constructs containing the sequence between nt -2400 and -86, but not for shorter constructs. An analysis of 14 kb of genomic sequence revealed the presence of five DNase I hypersensitive sites associated with active gene transcription. Three of the sites are located in the vicinity of the transcriptional start site and could be linked to the minimal promoter activity. Two of the sites are located downstream of the exon containing the start codon, suggesting the presence of intronic regulatory elements.

scholarly journals Characterization of the promoter of human adipocyte hormone-sensitive lipase

1998 ◽  
Vol 330 (3) ◽  
pp. 1481-1481
Author(s):  
J. GROBER ◽  
H. LAURELL ◽  
R. BLAISE ◽  
B. FABRY ◽  
S. SCHAAK ◽  
...  
Keyword(s):  
Hormone Sensitive Lipase ◽  
Human Adipocyte ◽  
Hormone Sensitive

scholarly journals Lipe gene polymorphism c.442 G>A influence on carcass traits in pigs

2018 ◽  
Vol 34 (1) ◽  
pp. 33-40
Author(s):  
Nijolė Peciulaitienė ◽  
Ilona Miceikienė ◽  
Natalija Makstutienė ◽  
Ramutė Miseikienė ◽  
Kristina Morkūnienė ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Genomic Dna ◽  
Carcass Traits ◽  
Fat Content ◽  
Hormone Sensitive Lipase ◽  
Expected Heterozygosity ◽  
Hormone Sensitive ◽  
Chelex Resin

Hormone sensitive lipase is one of three enzymes involved in lipolysis process and encoded by LIPE gene. In this study we investigated LIPE gene polymorphism c.442 G>A influence on carcass traits in hybrid pigs. Genomic DNA extracted using Chelex resin, genotypes determined using RFLP-PCR. Allele A observed with frequency 0,738, allele G - 0,262. The most common genotype was AA, genotype GG was observed with lower frequency, genotype AG was rarest. While evaluating population heterozygosity, it was noticed that observed heterozygosity was only 0,075, while expected heterozygosity was 0,387. In observed pig population allele A is associated with better animal muscularity, allele G - with greater fat content.

scholarly journals Hormone-sensitive lipase of rat adipose tissue. Purification and some properties.

1981 ◽  
Vol 256 (12) ◽  
pp. 6311-6320
Author(s):  
G. Fredrikson ◽  
P. Strålfors ◽  
N.O. Nilsson ◽  
P. Belfrage
Keyword(s):  
Adipose Tissue ◽  
Hormone Sensitive Lipase ◽  
Hormone Sensitive ◽  
Rat Adipose Tissue

scholarly journals Testis Expression of Hormone-sensitive Lipase Is Conferred by a Specific Promoter That Contains Four Regions Binding Testicular Nuclear Proteins

1999 ◽  
Vol 274 (14) ◽  
pp. 9327-9334 ◽  
Author(s):  
Régis Blaise ◽  
Jacques Grober ◽  
Philippe Rouet ◽  
Geneviève Tavernier ◽  
Dominique Daegelen ◽  
...  
Keyword(s):  
Hormone Sensitive Lipase ◽  
Nuclear Proteins ◽  
Hormone Sensitive

Hormone-sensitive lipase: sequence, expression, and chromosomal localization to 19 cent-q13.3

Science ◽  
1988 ◽  
Vol 241 (4872) ◽  
pp. 1503-1506 ◽  
Author(s):  
C Holm ◽  
T. Kirchgessner ◽  
K. Svenson ◽  
G Fredrikson ◽  
S Nilsson ◽  
...  
Keyword(s):  
Chromosomal Localization ◽  
Hormone Sensitive Lipase ◽  
Hormone Sensitive

scholarly journals Effects of Physiological Hypercortisolemia on the Regulation of Lipolysis in Subcutaneous Adipose Tissue1

1998 ◽  
Vol 83 (2) ◽  
pp. 626-631 ◽  
Author(s):  
Jaswinder S. Samra ◽  
Mo L. Clark ◽  
Sandy M. Humphreys ◽  
Ian A. MacDonald ◽  
Peter A. Bannister ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Sodium Succinate ◽  
Hormone Sensitive Lipase ◽  
Whole Body ◽  
Constant Infusion ◽  
Nonesterified Fatty Acid ◽  
Significant Difference ◽  
Hormone Sensitive ◽  
Subcutaneous Adipose

Cortisol is known to increase whole body lipolysis, yet chronic hypercortisolemia results in increased fat mass. The main aim of the study was to explain these two apparently opposed observations by examining the acute effects of hypercortisolemia on lipolysis in subcutaneous adipose tissue and in the whole body. Six healthy subjects were studied on two occasions. On one occasion hydrocortisone sodium succinate was infused iv to induce hypercortisolemia (mean plasma cortisol concentrations, 1500 ± 100 vs. 335± 25 nmol/L; P < 0.001); on the other occasion (control study) no intervention was made. Lipolysis in the sc adipose tissue of the anterior abdominal wall was studied by measurement of arterio-venous differences, and lipolysis in the whole body was studied by constant infusion of[ 1,2,3-2H5]glycerol for measurement of the systemic glycerol appearance rate. Hypercortisolemia led to significantly increased arterialized plasma nonesterified fatty acid (NEFA; P < 0.01) and blood glycerol concentrations (P < 0.05), with an increase in systemic glycerol appearance (P < 0.05). However, in sc abdominal adipose tissue, hypercortisolemia decreased veno-arterialized differences for NEFA (P < 0.05) and reduced NEFA efflux (P < 0.05). This reduction was attributable to decreased intracellular lipolysis (P < 0.05), reflecting decreased hormone-sensitive lipase action in this adipose depot. Hypercortisolemia caused a reduction in arterialized plasma TAG concentrations (P < 0.05), but without a significant change in the local extraction of TAG (presumed to reflect the action of adipose tissue lipoprotein lipase). There was no significant difference in plasma insulin concentrations between the control and hypercortisolemia study. Site-specific regulation of the enzymes of intracellular lipolysis (hormone-sensitive lipase) and intravascular lipolysis (lipoprotein lipase) may explain the ability of acute cortisol treatment to increase systemic glycerol and NEFA appearance rates while chronically promoting net central fat deposition.

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