scholarly journals Isolation and characterization of 10 polymorphic microsatellite loci in the rarest European damselfly, Coenagrion hylas (Odonata: Coenagrionidae)

2021 ◽  
Vol 24 ◽  
pp. 227-232
Author(s):  
M. Landmann ◽  
M. Schilling ◽  
A. Landmann ◽  
F. M. Steiner ◽  
B. C. Schlick-Steiner
Keyword(s):  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
Population Connectivity ◽  
Vulnerable Species ◽  
Central European ◽  
Limited Distribution ◽  
Isolation And Characterization ◽  
Polymorphic Microsatellite ◽  
European Populations

Within Europe, the damselfly Coenagrion hylas has a very limited distribution and is regarded as a vulnerable species. For studying migration and population connectivity in the Central European populations, 10 microsatellite markers were developed for this species. The loci were screened on 24 individuals collected at Lech valley, Tyrol, Austria. The values for expected and observed heterozygosity ranged from 0.192 to 0.802 and from 0.208 to 0.917, respectively. All developed markers were polymorphic.

scholarly journals Isolation and Characterization of Twenty Polymorphic Microsatellite Loci for Moringa oleifera (Moringaceae)

HortScience ◽  
2010 ◽  
Vol 45 (4) ◽  
pp. 690-692 ◽  
Author(s):  
Jiang-Chong Wu ◽  
Jing Yang ◽  
Zhi-Jian Gu ◽  
Yan-Ping Zhang
Keyword(s):  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
Population Genetic ◽  
Moringa Oleifera ◽  
Microsatellite Primers ◽  
Genetic Studies ◽  
Isolation And Characterization ◽  
Hardy Weinberg Equilibrium ◽  
Polymorphic Microsatellite

By using a modified biotin-streptavidin capturing method, a total of 20 polymorphic microsatellite markers were developed from Moringa oleifera Lam. (Moringaceae), a useful multipurpose tree. Twenty-four domesticated individuals, with germplasms of India and Myanmar, were used to screen polymorphism of these 20 microsatellite markers. The number of alleles per locus ranged from two to six. The expected and observed heterozygosity varied from 0.3608 to 0.7606 and from 0.0000 to 0.8750, respectively. Seven loci were significantly deviated from Hardy-Weinberg equilibrium. The availability of these microsatellite primers would provide a powerful tool for aspects of detailed population genetic studies of M. oleifera.

scholarly journals Isolation and Characterization of Polymorphic Microsatellite Markers for Two Subterranean Termites

Sociobiology ◽  
2017 ◽  
Vol 64 (3) ◽  
pp. 352 ◽  
Author(s):  
Yu-Lei Dang ◽  
Hong-Gui Zhang ◽  
Yu-Feng Meng ◽  
Min Zhang ◽  
Sha Zhao ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
Population Genetic ◽  
Subterranean Termites ◽  
Polymorphic Markers ◽  
Isolation And Characterization ◽  
Expected Heterozygosity ◽  
Hardy Weinberg Equilibrium ◽  
Polymorphic Microsatellite

We isolated 15 and 18 highly polymorphic genomic microsatellite markers from two subterranean termites, Reticulitermes aculabialis and R. labralis, respectively. A total of 53 alleles were detected in 15 microsatellite loci of R. aculabialis, and the alleles were 3.533±1.302 (mean±SD), while the corresponding data of R. labralis were 115 detected alleles in 18 microsatellite loci with 6.389±1.754 alleles. The observed and expected heterozygosity was 0.496±0.236 and 0.564±0.125 in R. aculabialis, and 0.368±0.263 and 0.702±0.115 in R. labralis, respectively. Seven loci were highly polymorphic (PIC>0.5) in R. aculabialis, and 15 loci were highly polymorphic (PIC>0.5) in R. labralis. All loci showed Hardy–Weinberg equilibrium. These polymorphic markers provide useful tools for population genetic and breeding system studies of subterranean termites.

scholarly journals Isolation and Characterization of Polymorphic Microsatellite Markers in Calocedrus macrolepis Kurz (Cupressaceae)

HortScience ◽  
2010 ◽  
Vol 45 (1) ◽  
pp. 169-171 ◽  
Author(s):  
Sheng-Xi Liao ◽  
Xian-Jie Mi ◽  
Ai-Zhong Liu ◽  
Kun Li ◽  
Zhen-Yin Yang ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Conservation Strategy ◽  
Vulnerable Species ◽  
Genetic Population ◽  
Isolation And Characterization ◽  
Geographical Populations ◽  
Polymorphic Microsatellite ◽  
Significant Linkage ◽  
Current Report

The Chinese Incense-cedar (Calocedrus macrolepis Kruz), an important wood and ornamental tree, is native to southwest China and also in northern Vietnam, Laos, Thailand, and Myanmar. As a result of ecological degradation in these areas, Chinese Incense-cedar was considered a vulnerable species according to the criteria of the International Union for the Conservation of Nature and Natural Resources. In the current report, we developed and characterized 13 novel microsatellite markers for this species using the protocol of fast isolation by amplified fragment length polymorphism of sequences containing repeats. Polymorphism of each locus was assessed in 36 individuals from nine geographical populations. The number of alleles per locus ranged from two to nine with an average of 6.08. The observed and expected heterozygosities ranged from 0.0000 to 1.0000 and from 0.1549 to 0.8912 with averages of 0.6688 and 0.6815, respectively. Four of the 13 loci were significantly deviated from Hardy-Weinberg expectations. No significant linkage disequilibrium was detected. These polymorphic microsatellite markers would be useful tools for investigating genetic population structure and diversity to establish conservation strategy for this interesting and vulnerable species.

Isolation and characterization of fourteen polymorphic microsatellite loci for Odontobutis sinensis

2014 ◽  
Vol 6 (3) ◽  
pp. 711-713
Author(s):  
Ping Qiu ◽  
Xuefen Yang ◽  
Kai-Jian Wei ◽  
Zhihong Ma ◽  
Ruibin Yang
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization ◽  
Polymorphic Microsatellite
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