Isolation and Characterization of Polymorphic Microsatellite Markers for Two Subterranean Termites

By using a modified biotin-streptavidin capturing method, a total of 20 polymorphic microsatellite markers were developed from Moringa oleifera Lam. (Moringaceae), a useful multipurpose tree. Twenty-four domesticated individuals, with germplasms of India and Myanmar, were used to screen polymorphism of these 20 microsatellite markers. The number of alleles per locus ranged from two to six. The expected and observed heterozygosity varied from 0.3608 to 0.7606 and from 0.0000 to 0.8750, respectively. Seven loci were significantly deviated from Hardy-Weinberg equilibrium. The availability of these microsatellite primers would provide a powerful tool for aspects of detailed population genetic studies of M. oleifera.

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Microsatellite markers for barnacle studies: Isolation and characterization of polymorphic microsatellite loci from the invasive barnacle Megabalanus coccopoma (Darwin, 1854) and its cross-amplification in the Southern Atlantic endemic species Megabalanus vesiculosus (Darwin, 1854)

Biochemical Systematics and Ecology ◽

10.1016/j.bse.2016.04.006 ◽

2016 ◽

Vol 66 ◽

pp. 224-228 ◽

Cited By ~ 1

Author(s):

Nívia Maria Nunes Abreu ◽

Isabela Marçal ◽

Angela Borges Duarte ◽

Fabio Bettini Pitombo ◽

Anderson Vilasboa ◽

...

Keyword(s):

Microsatellite Markers ◽

Endemic Species ◽

Microsatellite Loci ◽

Cross Amplification ◽

Isolation And Characterization ◽

Polymorphic Microsatellite

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Isolation and Characterization of Microsatellite Loci for the Ornamental Plant Primula obconica Hance (Primulaceae)

HortScience ◽

10.21273/hortsci.45.2.314 ◽

2010 ◽

Vol 45 (2) ◽

pp. 314-315 ◽

Cited By ~ 2

Author(s):

Hai-fei Yan ◽

Xue-jun Ge ◽

Chi-ming Hu ◽

Gang Hao

Keyword(s):

Genetic Variation ◽

Genetic Structure ◽

Microsatellite Markers ◽

Microsatellite Loci ◽

Ornamental Plant ◽

Microsatellite Variation ◽

Isolation And Characterization ◽

Expected Heterozygosity ◽

Fiasco Protocol

Nine microsatellite loci were isolated from Primula obconica using the FIASCO protocol. We used 30 individuals from three populations for the assessment of microsatellite variation. Seven loci were detected with microsatellite polymorphism. The number of alleles per locus ranged from three to seven. The average observed heterozygosity and expected heterozygosity ranged from 0.167 to 0.6 and from 0.409 to 0.653, respectively. These microsatellite markers will be useful to assess the genetic variation and genetic structure of P. obconica.

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Development and characterization of 29 polymorphic microsatellite loci of Megalobrama pellegrini by next-generation sequencing technology and cross-species amplification in related species

10.7287/peerj.preprints.2490 ◽

2016 ◽

Author(s):

Wen Song ◽

Dongmei Zhu ◽

Yefeng Lv ◽

Weimin Wang

Keyword(s):

Microsatellite Markers ◽

Microsatellite Loci ◽

Related Species ◽

Polymorphic Information Content ◽

Sequencing Technology ◽

Next Generation Sequencing Technology ◽

Expected Heterozygosity ◽

Polymorphic Microsatellite ◽

Generation Sequencing

Megalobrama pellegrini is one of the economically important freshwater fish in China. Here, we developed 29 polymorphic microsatellite loci of M. pellegrini. The number of alleles (Na), effective number of alleles (Ne), observed heterozygosity (HO), expected heterozygosity (HE) and polymorphic information content (PIC) ranged from 3 to 11 (mean±SD 5.4828±1.9571), 2.8708 to 9.6257 (mean±SD 5.0865±1.6681), 0.4333 to 0.9333 (mean±SD 0.7874±0.1213), 0.6627 to 0.9113 (mean±SD 0.7946±0.0751) and 0.5785 to 0.8868 (mean±SD 0.7439±0.0950), respectively. Cross-species amplification was successful at most loci for related species such as M. amblycephala, M. hoffmanni, M. skolkovii and Parabramis pekinensis. The transferability rate of the 29 polymorphic microsatellite markers in M. amblycephala, M. hoffmanni, M. skolkovii and P. pekinensis were 96.55%, 86.21%, 86.21% and 75.86%, respectively. These polymorphic microsatellites are not only useful in genetic study and conservation of M. pellegrini, but also an effective tool for identifying the related species. We could use 5 microsatellite markers (HHF-63, HHF-104, HHF-113, HHF-148, HHF-163) to distinguish the 5 species.

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Isolation and Characterization of 10 Polymorphic Microsatellite Loci in Paphiopedilum concolor (Batem.) Pfitzer (Orchidaceae) and Cross-species Amplification

HortScience ◽

10.21273/hortsci.45.8.1286 ◽

2010 ◽

Vol 45 (8) ◽

pp. 1286-1287 ◽

Cited By ~ 1

Author(s):

Long-na Li ◽

Songjun Zeng ◽

Feng Zheng ◽

Zhi-lin Chen ◽

Kun-lin Wu ◽

...

Keyword(s):

Genetic Structure ◽

Microsatellite Locus ◽

Microsatellite Loci ◽

Genomic Library ◽

Enriched Genomic Library ◽

Relationship Patterns ◽

Isolation And Characterization ◽

Expected Heterozygosity ◽

Polymorphic Microsatellite

Ten polymorphic microsatellite loci were isolated and characterized from an enriched genomic library of Paphiopedilum concolor (Batem.) Pfitzer. The number of alleles per microsatellite locus ranged from three to 11 with an average of 6.4 in a sample of 30 individuals from three populations. The observed and expected heterozygosity ranged from 0.200 to 0.800 and from 0.544 to 0.827, respectively. These microsatellites can be used as tools to investigate the genetic structure of P. concolor populations and relationship patterns with closely related taxa.

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Development and characterization of 29 polymorphic microsatellite loci of Megalobrama pellegrini by next-generation sequencing technology and cross-species amplification in related species

10.7287/peerj.preprints.2490v1 ◽

2016 ◽

Cited By ~ 1

Author(s):

Wen Song ◽

Dongmei Zhu ◽

Yefeng Lv ◽

Weimin Wang

Keyword(s):

Microsatellite Markers ◽

Microsatellite Loci ◽

Related Species ◽

Polymorphic Information Content ◽

Sequencing Technology ◽

Next Generation Sequencing Technology ◽

Expected Heterozygosity ◽

Polymorphic Microsatellite ◽

Generation Sequencing

Megalobrama pellegrini is one of the economically important freshwater fish in China. Here, we developed 29 polymorphic microsatellite loci of M. pellegrini. The number of alleles (Na), effective number of alleles (Ne), observed heterozygosity (HO), expected heterozygosity (HE) and polymorphic information content (PIC) ranged from 3 to 11 (mean±SD 5.4828±1.9571), 2.8708 to 9.6257 (mean±SD 5.0865±1.6681), 0.4333 to 0.9333 (mean±SD 0.7874±0.1213), 0.6627 to 0.9113 (mean±SD 0.7946±0.0751) and 0.5785 to 0.8868 (mean±SD 0.7439±0.0950), respectively. Cross-species amplification was successful at most loci for related species such as M. amblycephala, M. hoffmanni, M. skolkovii and Parabramis pekinensis. The transferability rate of the 29 polymorphic microsatellite markers in M. amblycephala, M. hoffmanni, M. skolkovii and P. pekinensis were 96.55%, 86.21%, 86.21% and 75.86%, respectively. These polymorphic microsatellites are not only useful in genetic study and conservation of M. pellegrini, but also an effective tool for identifying the related species. We could use 5 microsatellite markers (HHF-63, HHF-104, HHF-113, HHF-148, HHF-163) to distinguish the 5 species.

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Isolation and characterization of 10 polymorphic microsatellite loci in the rarest European damselfly, Coenagrion hylas (Odonata: Coenagrionidae)

International Journal of Odonatology ◽

10.23797/2159-6719_24_17 ◽

2021 ◽

Vol 24 ◽

pp. 227-232

Author(s):

M. Landmann ◽

M. Schilling ◽

A. Landmann ◽

F. M. Steiner ◽

B. C. Schlick-Steiner

Keyword(s):

Microsatellite Markers ◽

Microsatellite Loci ◽

Population Connectivity ◽

Vulnerable Species ◽

Central European ◽

Limited Distribution ◽

Isolation And Characterization ◽

Polymorphic Microsatellite ◽

European Populations

Within Europe, the damselfly Coenagrion hylas has a very limited distribution and is regarded as a vulnerable species. For studying migration and population connectivity in the Central European populations, 10 microsatellite markers were developed for this species. The loci were screened on 24 individuals collected at Lech valley, Tyrol, Austria. The values for expected and observed heterozygosity ranged from 0.192 to 0.802 and from 0.208 to 0.917, respectively. All developed markers were polymorphic.

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Characterization of new polymorphic microsatellite loci for the lesser tree frog (Dendropsophus minutus)

Amphibia-Reptilia ◽

10.1163/15685381-00002974 ◽

2015 ◽

Vol 36 (1) ◽

pp. 83-86

Author(s):

Hilton M. Oyamaguchi ◽

Ryo P. Okubo ◽

Hilton M. Oyamaguchi ◽

Ryo P. Okubo ◽

...

Keyword(s):

Microsatellite Loci ◽

Taxonomic Status ◽

Tree Frog ◽

Expected Heterozygosity ◽

Scale Population ◽

Hardy Weinberg Equilibrium ◽

Taxonomic Confusion ◽

Polymorphic Microsatellite ◽

Roche 454

Dendropsophus minutus is a widely distributed species in South America and presents great phenotypic variation which has led to taxonomic confusion. Herein we describe nine microsatellite loci for D. minutus discovered by genomic shotgun sequencing using Roche 454 pyrosequencing. These microsatellite loci were tested on a panel of 29 individuals. All nine loci were polymorphic (11-22 alleles) with observed heterozygosity ranging from 0.42 to 0.90 and expected heterozygosity from 0.75 to 0.94. Three loci deviated from the Hardy-Weinberg equilibrium. These characterized polymorphic markers for D. minutus will enable investigation of fine scale population structure, evolutionary processes, and clarify taxonomic status. This genetic information will be useful in conservation decisions regarding on this apparently wide distributed species.

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Development and Characterization of 68 Microsatellite Markers of Black Amur Bream Megalobrama terminalis by Next-Generation Sequencing

Turkish Journal of Fisheries and Aquatic Sciences ◽

10.4194/1303-2712-v21_6_05 ◽

2021 ◽

Vol 21 (06) ◽

pp. 299-308

Author(s):

Kai Liu ◽

Xiao-yu Feng ◽

Heng-jia Ma ◽

Nan Xie

Keyword(s):

Linkage Disequilibrium ◽

Next Generation Sequencing ◽

Microsatellite Markers ◽

Polymorphism Information Content ◽

Next Generation ◽

Expected Heterozygosity ◽

Hardy Weinberg Equilibrium ◽

Polymorphic Microsatellite ◽

Generation Sequencing

This study developed and characterized 68 novel polymorphic microsatellite markers from black Amur bream Megalobrama terminalis by next-generation sequencing. Variability was tested on 36 individuals collected from Qiantang River, Zhejiang Province, China. The number of alleles per locus ranged from 2 to 13. Observed heterozygosity ranged from 0.028 to 0.944, whereas the expected heterozygosity ranged from 0.028 to 0.887. Polymorphism Information Content ranged from 0.027 to 0.862. Moreover, 53 microsatellites were in agreement with Hardy–Weinberg equilibrium. Twenty-eight pairwise tests in 33 microsatellite loci indicated linkage disequilibrium. These microsatellites are a valuable tool for further genetics studies of this species.

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