scholarly journals Micropropagation and Field Evaluation of × Heucherella ‘Bridget Bloom’

1990 ◽  
Vol 8 (3) ◽  
pp. 156-159
Author(s):  
S.L. Kitto ◽  
J.J. Frett ◽  
P. Geiselhart
Keyword(s):  
Adventitious Shoots ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Field Evaluation ◽  
Axillary Shoot ◽  
Naphthaleneacetic Acid ◽  
Axillary Shoot Proliferation ◽  
Murashige Skoog ◽  
Micropropagated Plants ◽  
Bacto Agar

Abstract × Heucherella ‘Bridget Bloom’ shoots were surface disinfected and cultured on basal medium composed of Murashige-Skoog salts and vitamins and the following addenda per liter; sucrose, 30 g; glycine, 2 mg; and washed Difco Bacto-agar, 6 g. Axillary shoot proliferation was greatest on medium supplemented with 0.5 mg 1−1 benzyladenine, 0.025 mg 1−1 naphthaleneacetic acid and 4 g 1−1 washed Difco Bacto-agar. Adventitious shoots regenerated from callus that initiated from the base of cultured microcuttings. Microcuttings were rooted in Redi-Earth under intermittent mist for 4 wk (94% rooted) and then moved to a greenhouse (98% survival after 4 wk). During a 16 month field study, plants produced from microcuttings grew as well as, if not better than, greenhouse-grown plants propagated by division. Micropropagated plants originating from axillary buds had significantly greater fresh and dry weights, and initiated more leaves and crowns when grown under field conditions than plants originating from adventitious buds.

scholarly journals In Vitro Propagation of Eriostemon myoporoides and Eriostemon `Stardust'

HortScience ◽  
1994 ◽  
Vol 29 (6) ◽  
pp. 686-688 ◽  
Author(s):  
James R. Ault
Keyword(s):  
Root Length ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Length ◽  
Axillary Shoot ◽  
Naphthaleneacetic Acid ◽  
Stem Explants ◽  
Ms Medium ◽  
Axillary Shoot Proliferation

Optimal axillary shoot proliferation was obtained from stem explants of a clone of Eriostemon myoporoides DC. on Murashige and Skoog (MS) basal medium containing 0.1 mg BA/liter, and of Eriostemon `Stardust' on MS medium containing 0.5 mg BA/liter. Overall average number of shoots and shoot lengths for all treatments was greater for E. `Stardust' (22.4 shoots and 12.1-mm shoot length) than for E. myoporoides (4.5 shoots and 8.3-mm shoot length). Maximum percent rooting of E. myoporoides (42%) and E. `Stardust' (95%) was obtained on MS medium supplemented with 1.0 mg K-IBA/liter for E. myoporoides and 0.1 mg NAA/liter for E. `Stardust'. Overall average percent rooting and root lengths were greater for E. `Stardust' (42% rooting and 11.0-mm root length) than for E. myoporoides (27% rooting and 2.3-mm root length). For E. `Stardust', reducing sucrose in the rooting medium from 50 to 25 g·liter-1 significantly decreased overall average percent rooting to 1670 and root length to 6.8 mm. Plantlets of both clones were acclimatized in the greenhouse and transferred successfully to soil, although survival was <7070. Chemical names used: N -(phenylmethyl) -l H -purine-6-amine (BA); potassium-l H -indole-3-butyric acid (K-IBA); l-naphthaleneacetic acid (NAA).

scholarly journals In Vitro Propagation of an Endangered Helianthus verticillatus by Axillary Bud Proliferation

Plants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 712
Author(s):  
Marzena Nowakowska ◽  
Žaklina Pavlović ◽  
Marcin Nowicki ◽  
Sarah L. Boggess ◽  
Robert N. Trigiano
Keyword(s):  
Endangered Species ◽  
Shoot Proliferation ◽  
Genetic Fidelity ◽  
Induction Medium ◽  
Axillary Shoot ◽  
Axillary Shoot Proliferation ◽  
Regenerated Plants ◽  
Micropropagated Plants ◽  
Ssrs Markers

Helianthus verticillatus (Asteraceae), whorled sunflower, is a perennial species restricted to a few locations in the Southeastern United States. Habitat loss has caused H. verticillatus to become rare, and since 2014, it has been federally listed as an endangered species. As a part of the recovery plan for the restoration and protection of H. verticillatus, an efficient micropropagation protocol based on axillary shoot proliferation was developed. Various concentrations of 6-benzylaminopurine (BAP; 0 to 4.44 µM) were examined for their morphogenetic potential in the regeneration of six genotypes of H. verticillatus from the nodal explants derived from greenhouse-grown plants. Both the BAP concentration and genotype had significant effects on the regeneration capacity of H. verticillatus. Although the induced buds were observed on ½-strength Murashige and Skoog medium without plant growth regulators, a higher rate of induction and bud development were achieved on media with either 0.88 or 2.22 µM BAP, regardless of the genotype. Successful rooting of the induced shoots was achieved within four weeks after the transfer from the induction medium to the fresh ½-strength MS medium, but the rooting efficiency was dependent on the plant’s genetic background. Regenerated plantlets, with well-developed shoots and roots, were acclimatized successfully to greenhouse conditions with a 97% survival rate. Simple sequence repeats (SSRs) markers were employed to assess the genetic uniformity of the micropropagated plants of H. verticillatus. No extraneous bands were detected between regenerants and their respective donor plants, confirming the genetic fidelity and stability of regenerated plants. To our knowledge, the protocol developed in this study is the first such report for this endangered species.

scholarly journals Micropropagation of Clerodendrum phlomidis L.F.

2016 ◽  
Vol 24 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Mafatlal M. Kher ◽  
Deepak Soner ◽  
Neha Srivastava ◽  
Murugan Nataraj ◽  
Jaime A. Teixeira da Silva
Keyword(s):  
Callus Formation ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Nodal Explants ◽  
Axillary Shoot ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Axillary Shoot Proliferation ◽  
Clerodendrum Phlomidis

Abstract Clerodendrum phlomidis L. f. is an important medicinal plant of the Lamiaceae family, particularly its roots, which are used for various therapeutic purposes in a pulverized form. The objective of this study was to develop a standard protocol for axillary shoot proliferation and rooting of C. phlomidis for its propagation and conservation. Nodal explants were inoculated on Murashige and Skoog (MS) medium that was supplemented with one of six cytokinins: 6-benzyladenine, kinetin, thidiazuron, N6-(2-isopentenyl) adenine (2iP), trans-zeatin (Zea) and meta-topolin. Callus induction, which was prolific at all concentrations, formed at the base of nodal explants and hindered shoot multiplication and elongation. To avoid or reduce callus formation with the objective of increasing shoot formation, the same six cytokinins were combined with 4 μM 2,3,5-tri-iodobenzoic acid (TIBA) alone or in combination with 270 μM adenine sulphate (AdS). Nodal explants that were cultured on the medium supplemented with 9.12 μM Zea, 4 μM TIBA and 270 μM AdS produced significantly more and longer shoots than on medium without TIBA and AdS. Half-strength MS medium supplemented with 8.05 μM α-naphthaleneacetic acid was the best medium for root formation. Most (75%) in vitro rooted plantlets were successfully acclimatized under natural conditions.

scholarly journals In Vitro Propagation of Muscadine Grape by Axillary Shoot Proliferation

1990 ◽  
Vol 115 (2) ◽  
pp. 324-329 ◽  
Author(s):  
Ni Lee ◽  
Hazel Y. Wetzstein
Keyword(s):  
Basal Medium ◽  
Shoot Proliferation ◽  
Nodal Segments ◽  
Axillary Shoot ◽  
Axillary Shoot Proliferation ◽  
Vitis Rotundifolia ◽  
Shoot Production ◽  
Muscadine Grape ◽  
Better Than

Plantlets were recovered from axillary bud cultures of muscadine grape (Vitis rotundifolia, `Summit'). Nodal segments 0.5 to 1.0 cm long were cultured in Murashige and Skoog (MS) basal medium supplemented with 5, 10, 20, or 40 μm BA. Best total shoot production was obtained with 10 μm BA; with higher BA levels, shoots were unexpanded and exhibited high mortalities. MS medium supplemented with IBA enhanced rooting by increasing rooting percentage and number per plantlet. Shoots previously proliferated on medium with 5 μm BA rooted significantly better than those multiplied on 10 μM BA. Shoot vigor during rooting was greater in shoots proliferated on 5 vs. 10 μm BA. Root development was not significantly affected by liquid vs. agar-solidifted medium or shoot length. Chemical names used: N-(phenylmethyl) -1H-purin-6-amine (BA), 1H-indole-3-butyric acid (IBA).

scholarly journals In vitro Regeneration through Apical and Axillary Shoot Proliferation of Ficus religiosa L. - A Multi-purpose Woody Medicinal Plant

2010 ◽  
Vol 19 (1) ◽  
pp. 71-78 ◽  
Author(s):  
A.K.M. Sayeed Hassan ◽  
Farhana Afroz ◽  
Miskat Ara Akhter Jahan ◽  
Rahima Khatun
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Axillary Shoot ◽  
Axillary Shoot Proliferation ◽  
Ficus Religiosa ◽  
Half Strength

A protocol was established for mass propagation of the valuable medicinal plant Ficus religiosa L. (Moraceae) through in vitro culture using apical and axillary buds of young sprouts from selected plants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP + 0.1 mg/l IAA, in which 78 per cent of the explants produced 16 shoots per culture. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 24 shoots per culture. In vitro raised shoots rooted on half strength MS supplemented with 2.0 mg/l IBA + 0.1 mg/l NAA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for seven days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85 per cent.  Key words: Ficus religiosa, Medicinal plant, Shoot proliferation, Regeneration,                   Acclimatization D.O.I. 10.3329/ptcb.v19i1.4987 Plant Tissue Cult. & Biotech. 19(1): 71-78, 2009 (June)

scholarly journals A Laboratory Exercise for Axillary Shoot Proliferation using Ajuga reptans

1994 ◽  
Vol 4 (3) ◽  
pp. 312b-314 ◽  
Author(s):  
John E. Preece ◽  
Carl A. Huetteman
Keyword(s):  
Adventitious Shoots ◽  
Shoot Proliferation ◽  
Tween 20 ◽  
Axillary Shoot ◽  
Axillary Shoots ◽  
Single Node ◽  
Axillary Shoot Proliferation ◽  
Laboratory Exercise ◽  
Short Time ◽  
Shoot Tip Explants

This exercise was developed for a plant propagation course to demonstrate, in a short time, the four stages of micropropagation, the effects of cytokinin concentrations, and the differences between adventitious and axillary shoots. Greenhouse-grown stock plants were brought into the laboratory, and 4- to 5-cm-long tips of runners were surface-dis-infested for 15 min in 0.5% NaClO with 1 ml of Tween 20/liter, followed by two 5-min rinses in sterile water. Working in the open laboratory near the bases of pairs of lit Bunsen burners, students placed either single-node or shoot tip explants (2 cm long, five replications) onto MS medium with 0, 1, or 10 μM BA. Cultures were in-cubated in parafilm-sealed culture tubes on open laboratory benches. Axillary shoots grew regardless of concentration of BA, and explants on medium with 10 μM BA produced the most callus and adventitious shoots. Microshoots were rooted and ac-climatized under mist in the greenhouse. This exercise can be performed in an open laboratory without the use of laminar flow hoods, specialized sterilizing equipment, or supplemental lighting.

Axillary Shoot Proliferation of Adult Eastern Black Walnut

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 478c-478 ◽  
Author(s):  
Amy B. Bailey ◽  
John E. Preece ◽  
J.W. Van Sambeek
Keyword(s):  
Woody Plant ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Black Walnut ◽  
Axillary Shoot ◽  
Axillary Shoots ◽  
Axillary Shoot Proliferation ◽  
Shoot Number ◽  
Callus Production ◽  
Eastern Black Walnut

Softwood shoots of adult Juglans nigra (eastern black walnut) were forced from latent (epicormic) buds below the bark of large stem sections in a greenhouse. Once sufficiently long, the shoots were excised, surface-disinfested, and cut into 1-cm-long nodal or apical segments for establishment in culture vessels. Two experiments were conducted, each using explants of three different clones. The first experiment compared the effect of cytokinins: 0.3, 1.0, or 3.0 μM benzyladenine (BA) and 0.1, 0.3, or 1.0 μM thidiazuron (TDZ) arranged factorially. The basal medium was agar-solidified Long and Preece (LP) with 0.05 μM indole-3-butyric acid (IBA) and 30 g/L sucrose. The second experiment compared the agar-solidified basal media: Driver-Kuniyuki-Walnut (DKW), Woody Plant Medium (WPM) and LP, all with 1.0 μM BA, 0.3 μM TDZ, 0.05 μM IBA, and 30 g/L sucrose. Regardless of the BA concentration, explants on media containing 0.1 μM TDZ produced few, if any, axillary shoots while explants on media containing 1.0 μM TDZ excessive amounts of callus. Explants in media containing 0.3 μM TDZ, at all levels of BA, produced the greatest number of shoots and minimal callus. Male catkins were produced by 17 explants on various media. Fifteen of the catkin-producing explants were from one walnut clone. Axillary shoot number and callus production were not significantly affected by basal medium for any of the three clones.

scholarly journals Micropropagation of Helleborus orientalis Lam. and Aconitum uncinatum Linn. (Ranunulaceae)

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 871D-871 ◽  
Author(s):  
C.C. Lim ◽  
S.L. Kitto
Keyword(s):  
Ascorbic Acid ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Axillary Shoot ◽  
Field Plot ◽  
Axillary Shoots ◽  
Axillary Shoot Proliferation ◽  
Active Radiation ◽  
Helleborus Orientalis

The objectives of this study were to develop systems for mass proliferation, rooting, and reestablishment of microcuttings of Helleborus orientalis and Aconitum uncinatum. Basal medium for H. orientalis contained 1/2 MS with 0.4 mg thiamine–HCl/liter, 0.5 mg pyridoxine–HCI/liter, 0.5 mg nicotinic acid/liter, 100 mg i-inositol/liter, 5 mg BA/liter, 2% sucrose, and 0.7% Phytagar. There was no effect of GA (1 mg–liter–1) or TDZ (0.1, 1 mg–liter–1) on axillary shoot proliferation. Helleborus orientalis rooted in vermiculite, Redi-Earth, or 4 perlite: 1 peat with 50% to 56% survival. A field plot containing 18 clonal H. orientalis has been established. Basal medium for A. uncinatum contained WPM with 2% sucrose, 2.5 mg BA/liter, 150 ppm ascorbic acid, 150 ppm citric acid, and 0.7% Phytagar. There was no effect of photoperiod (8, 12, 14 h, 52.5 μmol–m–2–s–1 photosynthetic active radiation) or banana extract on axillary shoot proliferation. Significantly more axillary shoots were generated in the presence of BA (10 mg–liter–1) + kinetin (10 mg–liter–1). Medium containing 500 ppm of PVPP resolved blackening of microcutting bases. More than 500 in vitro-rooted microcuttings (1 mg IBA/liter) survived and grew when transplanted into MetroMix 510 and placed under humidity domes for 6 weeks in the mist.

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