The weaker sex: Male lingcod (Ophiodon elongatus) with blue color polymorphism are more burdened by parasites than are other sex–color combinations

The unusual blue color polymorphism of lingcod (Ophiodon elongatus) is the subject of much speculation but little empirical research; ~20% of lingcod individuals exhibit this striking blue color morph, which is discrete from and found within the same populations as the more common brown morph. In other species, color polymorphisms are intimately linked with host–parasite interactions, which led us to ask whether blue coloration in lingcod might be associated with parasitism, either as cause or effect. To test how color and parasitism are related in this host species, we performed parasitological dissection of 89 lingcod individuals collected across more than 26 degrees of latitude from Alaska, Washington, and California, USA. We found that male lingcod carried 1.89 times more parasites if they were blue than if they were brown, whereas there was no difference in parasite burden between blue and brown female lingcod. Blue individuals of both sexes had lower hepatosomatic index (i.e., relative liver weight) values than did brown individuals, indicating that blueness is associated with poor body condition. The immune systems of male vertebrates are typically less effective than those of females, due to the immunocompromising properties of male sex hormones; this might explain why blueness is associated with elevated parasite burdens in males but not in females. What remains to be determined is whether parasites induce physiological damage that produces blueness or if both blue coloration and parasite burden are driven by some unmeasured variable, such as starvation. Although our study cannot discriminate between these possibilities, our data suggest that the immune system could be involved in the blue color polymorphism–an exciting jumping-off point for future research to definitively identify the cause of lingcod blueness and a hint that immunocompetence and parasitism may play a role in lingcod population dynamics.

Design, construction and testing of a floating hood biodigester prototype for municipal waste organic waste

This paper documented the design, construction and operation test of a floating hood biodigester prototype, using organic residues (ruminal content, blood, bovine excreta and viscera) from the municipal trail of Ciudad Valles, S.L.P., with the purpose of generating biogas. The components of the biodigester system considered were: loading duct, concrete biodigester tank, biogas pipeline, floating hood, gas reservoir, discharge duct and discharge tank. A biodigester with storage capacity in the 0.178 m3 floating hood was designed for a 30-day trial operation and storage of 0.120 m3 of organic waste mixture in the biodigester tank. As of day 17 of operation the daily average of biogas generated was 0.1801 m3. The composition of the biogas at day 30 of operation, showed a content of 59.4% of CH4. When performing the flame test, an intense blue coloration was obtained, which indicates that the biogas produced has a high calorific value that will allow heating and flammability.

A Study on the Blue Coloration of Ao-ike Pond, Aomori Prefecture, Japan: Formulation of a Physical Model in Terms of Radiance and Image Analyses

The blue coloration model of a closed pond, Ao-ike Pond, Aomori Prefecture, Japan, was formulated in terms of radiance by applying a theory of observation devices proposed by Szirmay-Kalos (2008) and Hanaishi’s reverse ray tracing method. In this model, three potential contributions to the coloration were considered; irregular reflection at the Lambertian pond bottom, density fluctuation scattering by water, and Mie scattering by suspended solids. By utilizing model formulas for these mechanisms, some parameters were determined in order to duplicate the images of the pond surface without solar shading by tree leaves above the pond surface, in addition to the images with sunbeam trajectories by solar radiations passing through tree leaves, which are emitted from the water and visible on the surface. Simulating the pictures of the pond surface and the sun-beam-image analyses revealed that the blue colorations of Ao-ike Pond are mainly produced (1) by the density fluctuation scattering of water itself and the white Mie scattering by suspended solids and (2) by the red-light absorption by water in the optical paths before and after the two scatterings. Then, the density fluctuation scattering of water and the Mie scattering by suspended solids exhibited contributions of almost equal magnitude. The contribution of irregular reflections at the pond bottom was judged to be relatively small.

Heteragrion gorbi sp. nov. (Odonata: Heteragrionidae) from southeastern Brazil

Heteragrion gorbi sp. nov. (Zygoptera: Heteragrionidae) is described and diagnosed based on six ♂♂ and one ♀. The specimens were collected in a stream in a Neotropical savannah fragment in São Carlos, São Paulo, Brazil. We present pictures of the holotype and the female. This is a species with blue coloration pattern, rare among its congeners.

Establishment of Glyoxalase I gene transformation and regeneration of cotton (Gossypium hirsutum L.)

Aim: The current study was carried out to develop transgenic cotton plantlets with glyoxalase I (gly I) gene using Agrobacterium-mediated transformation. Methodology: Seeds of cotton were inoculated on MS medium and the explants such as shoot tip (3-5 mm), hypocotyl and leaf were aseptically removed from in vitro plantlets. The pre-cultured and infected explants with Agrobacterium harboring gly I gene and the shoot tip were inoculated on MS media for shoot initiation and subcultured on elongation medium with growth hormones, and antibiotics. Healthy and well-grown shoots were subcultured on medium with indole butyric acid (IBA) (0.3 mgl-1) for root formation and the plantlets were hardened in plastic cups with sterile soil. The putative transgenic plantlets were analyzed histochemically for gus gene and the PCR analysis was performed for gly 1 gene. Results: The transformation efficiency of cotton ranged 48.57 to 64.53 %. The regenerated plantlets showed the presence of gus gene in terms of blue coloration in shoots, whole leaf and leaf segments. The PCR was performed in putative transgenic plant lets with both gus gene as well as gly I gene primers. The PCR results showed the presence of 1031 bp DNA band with gus gene primers and 800 bp DNA band with the gly I gene primers. Interpretation: The current study has proven the reproducible procedure for the Agrobacterium-mediated gene transfer and regeneration of Indian cotton varieties. The PCR results revealed the presence of glyoxalase I gene in the transformants. Key words: Cotton varieties, Glyoxalase I gene, PCR analysis, Regeneration, Transformation

Blue flower coloration of Corydalis ambigua requires ferric ion and kaempferol glycoside

Corydalis ambigua (Japanese name, Ezoengosaku) flowers bloom with blue to purplish petals in early spring in Hokkaido prefecture. In this study, a mechanism for blue petal coloration by ferric ions and keampferol glycoside was elucidated. Blue petals and cell sap exhibited similar visible (Vis) spectra, with λmax at approximately 600 nm and circular dichroism (CD) with positive exciton-type Cotton effects in the Vis region. Analysis of the organic components of the petals confirmed cyanidin 3-O-sambubioside and kaempferol 3-O-sambubioside as the major flavonoids. Mg, Al, and Fe were detected in petals using atomic emission spectroscopy. Color, Vis absorption, and CD consistent with those of blue petals were reproduced by mixing cyanidin 3-O-sambubioside, kaempferol 3-O-sambubioside, and Fe3+ in a buffered aqueous solution at pH 6.5. Both Fe3+ and flavonol were essential for blue coloration.

The evolution of coloration and opsins in tarantulas

Tarantulas paradoxically exhibit a diverse palette of vivid coloration despite their crepuscular to nocturnal habits. The evolutionary origin and maintenance of these colours remains mysterious. In this study, we reconstructed the ancestral states of both blue and green coloration in tarantula setae, and tested how these colours correlate with presence of stridulation, urtication and arboreality. Green coloration has probably evolved at least eight times, and blue coloration is probably an ancestral condition that appears to be lost more frequently than gained. While our results indicate that neither colour correlates with the presence of stridulation or urtication, the evolution of green coloration appears to depend upon the presence of arboreality, suggesting that it ptobably originated for and functions in crypsis through substrate matching among leaves. We also constructed a network of opsin homologues across tarantula transcriptomes. Despite their crepuscular tendencies, tarantulas express a considerable diversity of opsin genes—a finding that contradicts current consensus that tarantulas have poor colour vision on the basis of low opsin diversity. Overall, our findings raise the possibility that blue coloration could have ultimately evolved via sexual selection and perhaps proximately be used in mate choice or predation avoidance due to possible sex differences in mate-searching.

A Case of Blue Coloration and Color Change in Pelophylax esculentus (Linnaeus, 1758) (Anura: Ranidae) in Denmark

One blue female Pelophylax esculentus was recorded in the outskirts of Copenhagen, Denmark, in August 2017. Minor green areas persisted. After capture and subsequent handling its base coloration darkened from what was first observed. No other individuals with blue coloration were found. Blue coloration is an anomaly due to lack of or reduction of xanthophores. This is a first record of truly blue-colored P. esculentus in North Europe though two cases from Central Europe are known. Within the genus Pelophylax blue individuals have been reported in various species at irregular intervals. The darkening agent α-melanocyte stimulating hormone (α-MSH) may well have been the reason why this individual turned darker after handling.

Pseudomonas azotoformans Belonging to Pseudomonas fluorescens Group as Causative Agent of Blue Coloration in Carcasses of Slaughterhouse Rabbits

The study describes the finding of an abnormal blue-tinged color found on rabbit carcasses in the refrigeration cell of two butcher shops in Apulia Region. The carcasses were from an industrial rabbitry for production of meat with a regularly authorized slaughterhouse. Pseudomonas azotoformans, a microorganism included in Pseudomonas fluorescens group, was isolated from samples collected by the altered carcasses, showing the growth of uniform bacterial colonies with fluorescent pigmentation. The bacterium was also isolated from an additional water sample and from the labelling gun collected in the slaughterhouse, whilst the knives used for slaughtering resulted negative. Chromatic alteration was experimentally reproduced on new carcasses using a 108 cfu/mL bacterial suspension prepared with the isolated strain. Due to their resistance characteristics, members of P. fluorescens group are very difficult to eradicate once introduced into the production environment. Therefore, their presence, even if not considered a public health problem, should be monitored by food industry operators in self-control plans.