scholarly journals Quantitative Determination of Ellagic Acid and Gallic Acid in Geum Rivale L. and G. Urbanum L.

2015 ◽  
Vol 56 (2) ◽  
pp. 74-78 ◽  
Author(s):  
Aleksandra Owczarek ◽  
Monika Anna Olszewska ◽  
Jan Gudej
Keyword(s):  
Medicinal Plants ◽  
Gallic Acid ◽  
Ellagic Acid ◽  
Biological Activities ◽  
Reversed Phase ◽  
Herbal Remedies ◽  
Reversed Phase Hplc ◽  
Hydrolyzable Tannins ◽  
Rp Hplc

Abstract Hydrolyzable tannins and products of their hydrolysis, ellagic acid (EA) and gallic acid (GA), are important con-stituents of many medicinal plants and exhibit various biological activities. Geum rivale and G. urbanum are traditional herbal remedies rich in tannin compounds. The aim of the study was to quantitate free and total EA and GA in aerial and underground parts of G. rivale and G. urbanum. After optimization of extraction, both compounds were quantitated by reversed phase HPLC (RP-HPLC). EA was more abundant than GA in the inves-tigated material, and underground parts of G. rivale were the richest source of total EA and GA.

TLC-Densitometric and RP-HPLC Methods for Simultaneous Determination of Dexamethasone and Chlorpheniramine Maleate in the Presence of Methylparaben and Propylparaben

2017 ◽  
Vol 100 (1) ◽  
pp. 51-58
Author(s):  
Nehal F Farid ◽  
Ibrahim A Naguib ◽  
Radwa S Moatamed ◽  
Mohamed R El Ghobashy
Keyword(s):  
Particle Size ◽  
Quantitative Determination ◽  
Mobile Phase ◽  
Ammonium Acetate ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Chlorpheniramine Maleate ◽  
Ammonium Acetate Buffer ◽  
Rp Hplc

Abstract Validated simple, sensitive, and highly selective methods are applied for the quantitative determination ofdexamethasone and chlorpheniramine maleate in the presence of their reported preservatives (methylparaben and propylparaben), whether in pure forms or in pharmaceutical formulation. TLC is the first method, in which dexamethasone, chlorpheniramine maleate, methylparaben, and propylparaben are separated on silica gel TLC F254 plates using hexane–acetone–ammonia (5.5 + 4.5 + 0.5, v/v/v) as the developing phase. Separated bands are scanned at 254 nm over a concentration range of 0.1–1.7 and 0.4–2.8 μg/band, with mean ± SD recoveries of 99.12 ± 0.964 and 100.14 ± 0.962%, for dexamethasone and chlorpheniramine maleate, respectively. Reversed-phase HPLC is the second method, in which a mixture of dexamethasone and chlorpheniramine maleate, methylparaben, and propylparaben is separated on a reversed-phase silica C18 (5 μm particle size, 250 mm, 4.6 mm id) column using 0.1 M ammonium acetate buffer–acetonitrile (60 + 40, v/v, pH 3) as the mobile phase. The drugs were detected at 220 nm over a concentration range of 5–50 μg/mL, 2–90 μg/mL, 4–100 μg/mL, and 7–50μg/mL, with mean ± SD recoveries of 100.85 ± 0.905, 99.67 ± 1.281, 100.20 ± 0.906, and 99.81 ± 0.954%, for dexamethasone, chlorpheniramine maleate, methylparaben paraben, and propylparaben, respectively. The advantages of the suggested methods over previously reported methods are the ability to detect lower concentrations of the main drugs and to show better resolution of interfering preservatives; hence, these methods could be more reliable for routine QC analyses.

Development and Validation of a Reversed-Phase HPLC Method for the Quantitative Determination of Ten Polyphenols Extracted from Apple Peel

2016 ◽  
Vol 99 (2) ◽  
pp. 481-488 ◽  
Author(s):  
Junjian Ran ◽  
Huadi Sun ◽  
Mingming Zhu ◽  
Juan Chen ◽  
Ruixiang Zhao
Keyword(s):  
Ellagic Acid ◽  
Reversed Phase ◽  
Hplc Method ◽  
Reversed Phase Hplc ◽  
Accuracy And Precision ◽  
Apple Peel ◽  
Validation Parameters ◽  
Satisfactory Precision ◽  
Development And Validation

Abstract A method based on a reversed-phase HPLC method was established, optimized, and validated for the separation and quantitation of 10 polyphenols extracted from the peel of apple species. A bidentate reversed-phase C18 column was used as stationary phase, and an acidified water buffer and methanol were used as mobile phase. The polyphenols were well separated and detected using UV at 280 and 320 nm. Validation parameters, such as linearity, LOD, LOQ, accuracy, and precision, were acceptable for all 10 polyphenols. The proposed method has enough linearity with correlation coefficient >0.99 within the investigated range for all tested polyphenols. The LOD was 0.24 μg/mL for ellagic acid and <0.2 μg/mL for all other polyphenols. The LOQ was 9.39 × 10−2 μg/mL for chlorogenic acid, and ellagic acid, 2.82 × 10−2 μg/mL for caffeic acid and >0.1 μg/mL for all other polyphenols. Recovery was within the acceptable range from 98.38 to 100.39% for all polyphenols standards. Satisfactory precision was achieved for both intra- and interday assay, with RSD <2%. The method was successfully applied for simultaneous analysis of polyphenols from apple peel.

Determination of 5-hydroxymethyl-2-furaldehyde in royal jelly by a rapid reversed phase HPLC method

2013 ◽  
Vol 5 (19) ◽  
pp. 5010 ◽  
Author(s):  
Marco Ciulu ◽  
Roberta Farre ◽  
Ignazio Floris ◽  
Valeria M. Nurchi ◽  
Angelo Panzanelli ◽  
...  
Keyword(s):  
Royal Jelly ◽  
Reversed Phase ◽  
Hplc Method ◽  
Reversed Phase Hplc

Simultaneous Determination of Ibuprofen and Pseudoephedrine Hydrochloride in Pharmaceutical Tablets by Reversed-Phase HPLC

2009 ◽  
Vol 42 (18) ◽  
pp. 2951-2961 ◽  
Author(s):  
Marie-Hélène Langlois ◽  
Philippe Dallet ◽  
Tina Kauss ◽  
Jean-Pierre Dubost
Keyword(s):  
Simultaneous Determination ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Pharmaceutical Tablets

The determination of starting conditions for modifier optimization in reversed-phase HPLC

1987 ◽  
Vol 24 (1) ◽  
pp. 108-114 ◽  
Author(s):  
L. de Galan ◽  
D. P. Herman ◽  
H. A. H. Billiet
Keyword(s):  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Starting Conditions

scholarly journals Simultaneous Determination of Preservatives (Methyl Paraben and Propyl Paraben) in Sucralfate Suspension Using High Performance Liquid Chromatography

2011 ◽  
Vol 8 (1) ◽  
pp. 340-346 ◽  
Author(s):  
Rajesh M. Kashid ◽  
Santosh G. Singh ◽  
Shrawan Singh
Keyword(s):  
High Performance Liquid Chromatography ◽  
Simultaneous Determination ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Hplc Method ◽  
Reversed Phase Hplc ◽  
Methyl Paraben ◽  
Propyl Paraben

A reversed phase HPLC method that allows the separation and simultaneous determination of the preservatives methyl paraben (M.P.) and propyl paraben (P.P.) is described. The separations were effected by using an initial mobile phase of water: acetonitrile (50:50) on Inertsil C18 to elute P.P. and M.P. The detector wavelength was set at 205 nm. Under these conditions, separation of the two components was achieved in less than 10 min. Analytical characteristics of the separation such as precision, specificity, linear range and reproducibility were evaluated. The developed method was applied for the determination of preservative M.P. and P.P. at concentration of 0.01 mg/mL and 0.1 mg/mL respectively. The method was successfully used for determining both compounds in sucralfate suspension.

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