scholarly journals Antibiotic Resistance and Plasmid Profile of Vibrio Alginolyticus Strains Isolated from Cultured European Sea Bass (Dicentrarchus Labrax, L.)

2013 ◽  
Vol 57 (2) ◽  
pp. 173-177 ◽  
Author(s):  
Jale Korun ◽  
Mehmet Karaca

Abstract In the study, antimicrobial resistance and plasmid profile of Vibrio alginolyticus strains isolated from diseased European sea bass (Dicentrarchus labrax, L.) collected from a commercial farm located on the Agean Region coast of Turkey, were investigated. The agent was identified by classical biochemical tests and the API 20NE system. Bacterial strains were tested for resistance to different antimicrobials by disc agar diffusion method and minimal inhibitory concentration (MIC) at two different incubation temperatures (22 ± 2ºC and 28 ± 2ºC for 24 to 28 h), and screened for plasmid DNA by agarose gel electrophoresis. It was found that 15 strains were susceptible to kanamycin but they revealed resistance to ampicillin, bacitracin and streptomycin at 22ºC. The strains were resistant to bacitracin and streptomycin but 14 strains were found to be intermediately resistant. One strain was resistant to ampicillin at 28ºC; two strains showed sensitivity to kanamycin, whereas 12 strains were intermediately resistant, and one strain was resistant to the same antibiotic at 28ºC. The strains demonstrared susceptibility to low concentrations of chloramphenicol, kanamycin, and trimethoprim (MICs of ≤ 8 μg/mL) at 22ºC. The examined strains were found to be susceptible to chloramphenicol (MICs of ≤8 μg/mL) and trimethoprim (MICs of ≤8 μg/ mL) at 28ºC. The strains harboured 2-3 plasmids, with sizes ranging from 68 to 126 kb.

2019 ◽  
Vol 28 (2) ◽  
pp. 493-510 ◽  
Author(s):  
Mohsen Abdel-Tawwab ◽  
Eman Khalifa ◽  
Amany M. Diab ◽  
Mohamed A. Khallaf ◽  
Nashwa Abdel-Razek ◽  
...  

Aquaculture ◽  
2021 ◽  
Vol 535 ◽  
pp. 736379
Author(s):  
Amany M. Diab ◽  
Riad H. Khalil ◽  
Rasmia H.M. Abu Leila ◽  
Mahmoud M. Abotaleb ◽  
Mohamed A. Khallaf ◽  
...  

2019 ◽  
Vol 90 ◽  
pp. 317-327 ◽  
Author(s):  
Shay Ravid-Peretz ◽  
Angelo Colorni ◽  
Galit Sharon ◽  
Michal Ucko

Aquaculture ◽  
2021 ◽  
pp. 737257
Author(s):  
A. Samaras ◽  
A. Dimitroglou ◽  
S. Kollias ◽  
G. Skouradakis ◽  
I.E. Papadakis ◽  
...  

Chemosphere ◽  
2007 ◽  
Vol 67 (6) ◽  
pp. 1171-1181 ◽  
Author(s):  
L. Giari ◽  
M. Manera ◽  
E. Simoni ◽  
B.S. Dezfuli

2011 ◽  
Vol 75 (5) ◽  
pp. 643-649 ◽  
Author(s):  
Francesco Abbate ◽  
Maria Cristina Guerrera ◽  
Giuseppe Montalbano ◽  
Felix De Carlos ◽  
Alberto Álvarez Suárez ◽  
...  

Author(s):  
Malyka Galay-Burgos ◽  
Lynda Llewellyn ◽  
Constantinos C Mylonas ◽  
Adelino V.M Canario ◽  
Silvia Zanuy ◽  
...  

Author(s):  
Marinelle Espino ◽  
Harkaitz Eguiraun ◽  
Oihane Diaz de Cerio ◽  
José Antonio Carrero ◽  
Nestor Etxebarria ◽  
...  

AbstractFeeding 3.9 and 6.7 mg Hg/kg (Se/Hg molar ratios of 0.8 and 0.4, respectively) for 14 days negatively affected Dicentrarchus labrax growth and total DNTB- and thioredoxin-reductase (TrxR) activities and the transcription of four redox genes (txn1, gpx1, txnrd3, and txnrd2) in the liver, but a diet with 0.5 mg Hg/kg (Se/Hg molar ratio 6.6) slightly increased both reductase activities and the transcription of txn1, gpx1, and txnrd2. Feeding 6.7 mg Hg/kg for 53 days downregulated the genes of the thioredoxin system (txn1, txnrd3, and txnrd2) but upregulated gpx1, confirming the previously proposed complementarity among the antioxidant systems. Substitution of 20% of the feed by thawed white fish (hake) slightly counteracted the negative effects of Hg. The effects were not statistically significant and were dependent, in a non-linear manner, on the Se/Hg molar ratio of the feed but not on its Hg concentration. These results stress the need to consider the Se/Hg molar ratio of the feed/food when evaluating the toxicity of Hg.


2014 ◽  
Vol 4 (2) ◽  
Author(s):  
C. Lorin-Nebel ◽  
H. Budzinski ◽  
K. Le Ménach ◽  
M.H. Devier ◽  
G. Charmantier ◽  
...  

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