scholarly journals Wild Nicotiana Species as a Source of Cytoplasmic Male Sterility in Nicotianatabacum

2002 ◽  
Vol 20 (4) ◽  
pp. 301-311
Author(s):  
V Nikova ◽  
R Vladova
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Wild Species ◽  
Seed Set ◽  
Seed Proteins ◽  
Nuclear Interaction ◽  
Reproductive Organs ◽  
Normal Female ◽  
Male Sterile ◽  
Male Organ

AbstractThe results of our experiments executed to obtain tobacco male sterile lines through interspecific hybridization are summarized. Ten wild species from the genus Nicotiana: N. excelsior (exc), N. amplexicaulis (amp), N. rustica (rus), Nicotianaglauca (gla), N. velutina (vel), N. benthamiana (ben), N. maritima (mar), N. paniculata (pan), N. longiflora (lon) and N. africana (afr) were used as cytoplasmic donors and N. tabacum, cv. HarmanliiskaBasma (HB) as a donor of the nucleus. Genetic effects of cytoplasmic-nuclear interaction of the studied species are discussed. Our results suggested that cytoplasmic male sterility (CMS) was expressed when the cytoplasms of the above mentioned wild Nicotiana species were combined with the nucleus of N. tabacum. The 10 sources of CMS obtained in tobacco were characterized by altered flower phenotypes. Flowers are classified into types according the stamen, pistil and corolla modification. All these CMS sources were backcrossed to Oriental tobaccos, cvs. Tekne, Nevrokop B-12, Kroumovgrad 90 and Djebel 576, to develop corresponding CMS lines. The investigated cytoplasms produced compete male sterility in all those cultivars. The CMS lines preserved flower types, specific for every “sterile” cytoplasm. The extent of male organ modifications varied from apparently normal (but pollenless) stamens in CMS (pan), (afr), some plants of (vel) (mar) through different degrees of malformations (shriveled anther on shortened filaments (lon), pinnate-like anthers on filaments of normal length (amp), petal - (ben), pistil- or stigma-like structures (rus), (gla)) to lack of male reproductive organs in (exc) and in some plants of (vel), (mar), (rus) and (gla). Most of the above mentioned cytoplasms had normal female gametophyte and good seed productivity. Alterations of the pistils were observed in CMS (rus), (exc) and (ben) causing reduction of the seed set. Electrophoresis of seed proteins of the tobacco cultivars and their CMS lines also suggested that the nuclei of wild species was entirely displaced by the nucleus of N. tabacum. CMS lines with cytoplasms of N. velutina, N. maritima, N. paniculata, N. longiflora and N. amplexicaulis were selected as suitable for seed production in tobacco.

scholarly journals Intraspecific variations of the cytoplasmic male sterility genes orf108 and orf117 in Brassica maurorum and Moricandia arvensis, and the specificity of the mRNA processing

Genome ◽  
2021 ◽  
Author(s):  
Hiroshi Yamagishi ◽  
Ayako Hashimoto ◽  
Asumi Fukunaga ◽  
Sang Woo Bang ◽  
Toru Terachi
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Dna Sequences ◽  
Wild Species ◽  
Polymerase Chain Reaction Amplification ◽  
Mitochondrial Gene ◽  
Male Sterile ◽  
Intraspecific Variations ◽  
Moricandia Arvensis ◽  
Sterility Genes

The mitochondrial gene <i>orf108</i> co-transcribed with <i>atp1</i> and causes cytoplasmic male sterility in <i>Brassica</i> crops, is widely distributed across wild species and genera of <i>Brassicaceae</i>. However, intraspecific variations in the presence of <i>orf108</i> have not yet been studied, and the mechanisms for the wide distribution of the gene remain unclear. We analyzed the presence and sequence variations of <i>orf108</i> in two wild species, <i>Brassica maurorum</i> and <i>Moricandia arvensis</i>. After polymerase chain reaction amplification of the 5′ region of <i>atp1</i> and the coding sequence of <i>orf108</i>, we determined the DNA sequences. <i>B. maurorum</i> and <i>M. arvensis</i> showed variations for the presence of <i>orf108</i> or <i>orf117</i> (<i>orf108<sup>V117</sup></i>) both between and within accessions, and were not fixed to the mitochondrial type having the male sterile genes. Sequencing of the amplicons clarified that <i>B. maurorum</i> has <i>orf108<sup>V117</sup></i> instead of <i>orf108</i>. Sequencing also indicated mitochondrial heteroplasmy in the two species; particularly, in <i>B. maurorum</i>, one plant possessed both the <i>orf108</i> and <i>orf108<sup>V117</sup></i> sequences. The results suggested that substoichiometric shifting of the mitochondrial genomes leads to the acquisition or loss of <i>orf108</i>. Furthermore, fertility restorer genes of the two species were involved in the processing of the mRNA of the male sterility genes at different sites.

scholarly journals Investigations of IsogenomicAlloplasmics of Flue-Cured Tobacco Nicotianatabacum cv. Wislica

2005 ◽  
Vol 21 (5) ◽  
pp. 259-263
Author(s):  
A Berbec ◽  
D Laskowska
Keyword(s):  
Male Sterility ◽  
Leaf Area ◽  
Leaf Blade ◽  
Seed Set ◽  
Field Performance ◽  
Normal Female ◽  
Male Sterile ◽  
White Spots ◽  
Male Sterile Cytoplasm ◽  
Alien Cytoplasm

AbstractCytoplasms of fifteen wild Nicotianae and a male sterile cytoplasm (cms) from N. tabacum were bred into the genome of a standard Polish flue-cured cultivar Wislica. The sixteen iso-genomic alloplasmics were compared for expression of male sterility and for some traits related to field performance. In cmsN. bigelovii, N. debneyi, N. exigua, N. megalosiphonor N. suaveolens stamens were absent, in cmsN. tabacum, N. glauca, N. goodspeedii or N. undulata stamens were absent or rudimentary, in cmsN. eastii, N. glutinosa or N. plumbaginifoliastamen tissue became petaloid, in those from N. knightiana and N. raimondiithey were morphologically normal. Female organ morphology was changed in cmsN. goodspeedii, N. occidentalis, N. exigua, N. debneyior N. bigelovii, seed set was reduced only in cmsN. occidentalis. Plant height in most alloplasmics was similar to that of the fertile variety but was strongly depressed by the cytoplasms of N. glutinosa, N. eastiiand N. plumbaginifolia. Leaf area was positively affected by N. amplexicaulis, N. bigeloviiand N. undulatacytoplasms, unaffected by N. suaveolens, N. tabacumand N. glaucacytoplasms and negatively affected by the remaining cytoplasms mostly because of the narrowing of the leaf blade. Cured leaf yields from cmsN. bigelovii were higher than those of Wislica. Leaf yields from cmsN. amplexicaulis, N. suaveolens, N. glauca or N. tabacum were not affected by the alien cytoplasm, whereas those from the remaining alloplasmics were depressed to different extents. Increased incidence of PVY and white spots (mostly attributable to Cercospora sp.) were observed on many of the alloplasmics especially on cmsN. exigua and N. suaveolens(PVY) and increased white spots only on cmsN. bigelovii, N. exigua, N. occidentalis and N. undulata.

scholarly journals The Dynamics of Gynodioecy in Plantago lanceolatu L. II. Mode of Action and Frequencies of Restorer Alleles

Genetics ◽  
1997 ◽  
Vol 147 (3) ◽  
pp. 1317-1328
Author(s):  
Anita A de Haan ◽  
Hans P Koelewijn ◽  
Maria P J Hundscheid ◽  
Jos M M Van Damme
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Allele Frequency ◽  
Mode Of Action ◽  
Male Fertility ◽  
Fertility Restoration ◽  
Plantago Lanceolata ◽  
Allele Frequencies ◽  
Male Sterile ◽  
Male Fertility Restoration

Male fertility in Plantago lanceolata is controlled by the interaction of cytoplasmic and nuclear genes. Different cytoplasmic male sterility (CMS) types can be either male sterile or hermaphrodite, depending on the presence of nuclear restorer alleles. In three CMS types of P. lanceolata (CMSI, CMSIIa, and CMSIIb) the number of loci involved in male fertility restoration was determined. In each CMS type, male fertility was restored by multiple genes with either dominant or recessive action and capable either of restoring male fertility independently or in interaction with each other (epistasis). Restorer allele frequencies for CMSI, CMSIIa and CMSIIb were determined by crossing hermaphrodites with “standard” male steriles. Segregation of male steriles vs. non-male steriles was used to estimate overall restorer allele frequency. The frequency of restorer alleles was different for the CMS types: restorer alleles for CMSI were less frequent than for CMSIIa and CMSIIb. On the basis of the frequencies of male steriles and the CMS types an “expected” restorer allele frequency could be calculated. The correlation between estimated and expected restorer allele frequency was significant.

scholarly journals INHERITANCE OF FERTILITY RESTORATION INVOLVING WILD ABORTIVE CYTOPLASMIC MALE STERILITY SYSTEM IN RICE (Oryza sativa L.)

2011 ◽  
Vol 24 (1) ◽  
pp. 33-40
Author(s):  
M. J. Hasan ◽  
M. U. Kulsum ◽  
A. Ansari ◽  
A. K. Paul ◽  
P. L. Biswas
Keyword(s):  
Oryza Sativa ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Gene Action ◽  
Oryza Sativa L ◽  
Fertility Restoration ◽  
Dominant Gene ◽  
Recessive Gene ◽  
Male Sterile ◽  
Male Sterile Line

Inheritance of fertility restoration was studied in crosses involving ten elite restorer lines of rice viz. BR6839-41-5-1R, BR7013-62-1-1R, BR7011-37-1-2R, BR10R, BR11R, BR12R, BR13R, BR14R, BR15R and BR16R and one male sterile line Jin23A with WA sources of cytoplasmic male sterility. The segregation pattern for pollen fertility of F2 and BC1 populations of crosses involving Jin23A indicated the presence of two independent dominant fertility restoring genes. The mode of action of the two genes varied in different crosses revealing three types of interaction, i.e. epistasis with dominant gene action, epistasis with recessive gene action, and epistasis with incomplete dominance.DOI: http://dx.doi.org/10.3329/bjpbg.v24i1.16997

Cytoplasmic male sterility in Desmodium

1969 ◽  
Vol 20 (2) ◽  
pp. 227 ◽  
Author(s):  
KS McWhirter
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Interspecific Hybrid ◽  
Pollen Fertility ◽  
Pollen Sterility ◽  
Hybrid Origin ◽  
F1 Hybrids ◽  
Male Sterile ◽  
Genetic Stocks

A type of male sterility found in two Desmodium plants of probably interspecific hybrid origin was cytoplasmically inherited. The cytoplasmic male-sterile character was incorporated in the tropical legume Desmodium sandwicense by backcrossing. In this genetic background pollen sterility was complete. The male-sterile character was not graft-transmissible, and it produced no detectable pleiotropic effects on growth and development. Desmodium intortum gave restoration of pollen fertility in Fl hybrids with male-sterile lines of D. sandwicense. Restored F1 hybrids produced apparently normal pollen, but tests of functional ability of the pollen disclosed that pollen fertility was less than that of Fl hybrids with normal cytoplasm. Incomplete restoration of fertility was not due to heterozygosity of fertility-restoring genes with gametophytic expression, since fertility-restoring genes were shown to act sporophytically. The results established the occurrence in the legume Desmodium of a system of determination of the male-sterile, fertility-restored phenotypes that is similar to the cytoplasmic male sterility systems described in many other angiosperm plants. A scheme utilizing the genetic stocks produced in this study for commercial production of the interspecific hybrid D. sandwicense x D. intortum as a cultivar is presented.

The effect of genotype × cytoplasm interaction on meiotic behaviour of maize chromosomes

Genome ◽  
1992 ◽  
Vol 35 (4) ◽  
pp. 653-658 ◽  
Author(s):  
M. M. Kruleva ◽  
A. B. Korol ◽  
T. G. Dankov ◽  
V. G. Skorpan ◽  
I. A. Preygel
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Relative Stability ◽  
Chiasma Frequency ◽  
Chiasma Formation ◽  
Meiotic Behaviour ◽  
Male Sterile ◽  
Maize Hybrids ◽  
Male Sterile Cytoplasm ◽  
Cytoplasmic Sterility

The effect of four isogenic cytoplasmic types, normal, Salvador, Texas, and Charrua (the latter three causing male sterility), on the process of chiasma formation has been studied using two different maize hybrids. The cytoplasmic male sterility determinants have been shown to reduce the rate of interstitial exchanges per nucleus and per bivalent and the frequency of univalents. Increased variation between plants and relative stability of the intercellular variation within a plant have been observed for the parameters studied. It is concluded that the determinants of cytoplasmic sterility lower the probability of additional exchanges (relative to the obligate one) and reduce the frequency of premature disruption of one-exchange chromosome associations.Key words: maize, chiasma frequency, male sterile cytoplasm, univalent formation, genotype × cytoplasm interaction.

scholarly journals Cytoplasmic male sterility and inter and intra subgenomic heterosis studies in Brassica species: A review

2014 ◽  
Vol 59 (3) ◽  
pp. 207-226
Author(s):  
Valiollah Rameeh
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Agronomic Traits ◽  
Indian Mustard ◽  
Brassica Species ◽  
Interspecific Crosses ◽  
Male Sterile ◽  
Evolutionary Origins ◽  
Brassica Spp ◽  
Potential Use

Plants of the genus Brassica comprise a remarkably diverse group of crops and encompass varieties that are grown as oilseeds, vegetables, condiment mustards and forages. One of the basic requirements for developing hybrid varieties in oilseed Brassica is the availability of proven heterosis. The development of hybrid cultivars has been successful in many Brassica spp. Midparent heterosis and high-parent heterosis (heterobeltiosis) have extensively been explored and utilized for boosting various quantity and quality traits in rapeseed. Heterosis is commercially exploited in rapeseed and its potential use has been demonstrated in turnip rape (B. rapa L.) and Indian mustard (B. juncea L.) for seed yield and most of the agronomic traits. The oilseed rape plant, B. napus, possesses two endogenous male sterile cytoplasms, nap and pol. Ogura type of cytoplasmic male sterility was first discovered in Japanese wild radish and other male-sterile Brassicas (Ogura bearing cytoplasm) derived from interspecific crosses. Information concerning the allelic frequencies of restorers can be useful in trying to understand their evolutionary origins. The ogu, pol and nap cytoplasms of B. napus induce sterility in all, some, and only a few cultivars, respectively. In this study, different kinds of male sterility, combining ability and heterosis of qualitative and quantitative traits in different Brassica species will be revi?wed.

Sign in / Sign up

Export Citation Format

Share Document