Toxicity evaluation of Dolichandrone serrulata flower extract on vital and reproductive organs in adult male rats

Although Dolichandrone serrulata flower (DSF) aqueous extract has been shown to possess pharmacological properties, its systemic toxicity has still to be evaluated. The present study aimed to investigate the sub-chronic toxicity effect of DSF extract on biochemical parameters and histological structures of liver, kidney, testis, and epididymis plus vas deferens. Adult male rats were administered DSF at 100, 300, and 600 mg/kgBW via oral gavage for 48 consecutive days while control rats received distilled water. At the end of the experiment, blood, liver, kidney, testis, and epididymis plus vas deferens samples were collected to determine any changes to serum biochemical components including ALT, ALP, and creatinine levels and histological structures. The results revealed no significant difference in body weight and food or water consumption between control and the DSF-treated groups. It was found that DSF significantly increases the weight of epididymis plus vas deferens, while the kidney and liver showed a decrease in the high dose group (P value < 0.05). Histological changes in these vital and reproductive tissues including fibrosis were not observed after administration but ALT, ALP, and creatinine levels were significantly altered in the treated groups (P value < 0.05). These altered levels, however, were still within normal ranges. In conclusion, these findings demonstrated that D. serrulata flower extract had no sub-chronic toxicity on vital and reproductive structures but slightly altered some liver and kidney functions.

Morphogenesis of Anthers Isolated From Cabbage (Brassica Oleracea L.) In Vitro

This study aimed to optimize the steps of obtaining regenerated cabbage plants by direct embryogenesis from isolated anthers and ovaries. Stepwise pretreatment of inflorescences was usedfor the studied hybrids and inbred lines. First, the inflorescences were placed in water and kept at a temperature of +4-6∘C for one day without the use of biologically active substances. Then the inflorescences were placed in a solution of the drug Dropp (10 mg/l) and cultivated for two days. After that, the anthers and ovaries were isolated from the flower buds and cultured on the MS culture medium at a temperature of + 32∘C for one day. The cultivation of the isolated explants on a nutrient medium (containing 0.01 mg/lof Dropp, 1.0 mg/lof NAA, 500 mg/lof asparagine, 100 mg/l of tyrosine, and 10 g/l of sucrose)led to an increase in their morphogenetic potential in the culture of anthers and ovaries (by 3.42% and 5.54%, respectively).A cytological method was usedto demonstrate the haploid nature of the regenerating plants. The number of chromosomes in the root meristem andleaves, and the chloroplasts in the closing cells of the stomatawere calculated. Keywords: cabbage, culture in vitro, regenerated plants, anthers, ovaries, reproductive organs

Amelioration of ethanol reprotoxicity by aqueous Spinacia oleracea extract in male rabbits Oryctolagus cuniculus

Spinacia oleracea “Spinach” is a plant rich in antioxidants; it leads to improved male reproduction. For this reason, the objective of this study is to evaluate the effect of the aqueous extract of this plant on the fertility of adult male rabbits after their administration with ethanol. The rabbits were divided into four groups: control group, group treated with 150 mg / kg / day of aqueous Spinacia oleracea extract (Ep), group treated with 40% ethanol (EtOH), and group treated with 150 mg / kg / day of aqueous Spinacia oleracea extract + 40% ethanol (Ep + EtOH), for a period of five successive weeks. Treatment was administered orally. After sacrificing the rabbits, the reproductive organs were removed and weighed. Certain sperm parameters (concentration, mobility, speed, and vitality), the testosterone level and the weight of the testes and epididymis were assessed. The results obtained showed a decrease in the concentration, mobility, speed and vitality of sperm, accompanied by a decrease in testosterone levels, and in the weight of the testes and epididymis in the group treated with ethanol, on the other hand, treatment with aqueous Spinacia oleracea extract revealed an increase in these parameters. In conclusion, treatment with aqueous Spinacia oleracea extract can repair the damage caused by ethanol.

Asymmetry of the male internal reproductive organs in Mantophasmatodea

Background Asymmetries are a widespread phenomenon in otherwise bilaterally symmetric organisms, and investigation of asymmetric structures can help us gather insights into fundamental evolutionary processes such as the selection for morphological novelties caused by behavioural changes. In insects, asymmetric genitalia have evolved in almost every order, and usually it’s the sclerotized parts and most conspicuous male phallic organs that are known to exhibit asymmetries. While external copulatory organs in insects have often been subject to investigations concerning asymmetries and the evolution thereof, internal reproductive structures have received far less attention. Here we describe the internal and external male genitalia in three species of Austrophasmatidae, Mantophasmatodea, using μ-CT imaging and light microscopy. Mantophasmatodea is the most recently discovered insect order, and with 21 species described to date, it is among the smallest insect orders currently known. Results We confirm that male heelwalkers exhibit asymmetries in the external genitalia and associated structures, represented by asymmetric phallic lobes and cerci. Moreover, we found an extreme asymmetry within the internal male genitalia: in all adult males investigated (N = 5), the seminal vesicle, a dilatation of the vas deferens, was only developed on the right side of the male while missing on the left side. Conclusion The false-male-above mating position exhibited by Mantophasmatodea and especially the long copulation duration of ca. 3 days might select for this unusual absence asymmetry of the left seminal vesicle. If this holds true for all heelwalker species, this absence asymmetry constitutes another autapomorphy for Austrophasmatidae or even the insect order Mantophasmatodea.

Are Temperate Alpine Plants With Distinct Phenology More Vulnerable to Extraordinary Climate Events Than Their Continuously Flowering Relatives in Tropical Mountains?

Alpine plants are perceived as some of the most vulnerable to extinction due to the global climate change. We expected that their life history strategies depend, among others, on the latitude they live in: those growing in temperate regions are likely to have a distinct phenology with short seasonal peaks, while tropical alpine plants can potentially exploit favorable year-round growing conditions and different individuals within a population may flower at different times of the year. In species, whose flowering is synchronized into short seasonal peaks, extraordinary climate events, which may become stronger and more frequent with climate change, can potentially destroy reproductive organs of all synchronized individuals. This may result in reducing fitness or even extinction of such species. We studied field populations of five groups of closely related Andean alpine plant species to test our expectations on their latitude-dependent synchronization of flowering. Our results confirmed these expectations: (i) Tropical alpine species were least synchronized and flowering peaks of different individuals in their populations were distributed across many months. Thus, in tropical alpine species, if an extraordinary event happens, only some individuals are affected and other members of the population successfully reproduce in other parts of the long season. (ii) Higher synchronicity in flowering of temperate and subtropical alpine plants resulted even in some of these species using only a part of the short growing season to reproduce, which increases their vulnerability to extraordinary climatic events. However, we did not find any unique pattern valid for all species, groups and regions. The diversity in flowering phenology (i.e., different levels of seasonality and synchronicity) that we found increases the likelihood of plants successfully coping with climate change.

The Chromosome-Level Genome of Miracle Fruit (Synsepalum dulcificum) Provides New Insights Into the Evolution and Function of Miraculin

Miracle fruit (Synsepalum dulcificum) is a rare valuable tropical plant famous for a miraculous sweetening glycoprotein, miraculin, which can modify sour flavors to sweet flavors tasted by humans. Here, we present a chromosome-level high-quality genome of S. dulcificum with an assembly genome size of ∼550 Mb, contig N50 of ∼14.14 Mb, and 37,911 annotated protein-coding genes. Phylogenetic analysis revealed that S. dulcificum was most closely related to Camellia sinensis and Diospyros oleifera, and that S. dulcificum diverged from the Diospyros genus ∼75.8 million years ago (MYA), and that C. sinensis diverged from Synsepalum ∼63.5 MYA. Ks assessment and collinearity analysis with S. dulcificum and other species suggested that a whole-genome duplication (WGD) event occurred in S. dulcificum and that there was good collinearity between S. dulcificum and Vitis vinifera. On the other hand, transcriptome and metabolism analysis with six tissues containing three developmental stages of fleshes and seeds of miracle fruit revealed that Gene Ontology (GO) terms and metabolic pathways of “cellular response to chitin,” “plant–pathogen interaction,” and “plant hormone signal transduction” were significantly enriched during fruit development. Interestingly, the expression of miraculin (Chr10G0299340) progressively increased from vegetative organs to reproductive organs and reached an incredible level in mature fruit flesh, with an fragments per kilobase of transcript per million (FPKM) value of ∼113,515, which was the most highly expressed gene among all detected genes. Combining the unique signal peptide and the presence of the histidine-30 residue together composed the main potential factors impacting miraculin’s unique properties in S. dulcificum. Furthermore, integrated analysis of weighted gene coexpression network analysis (WGCNA), enrichment and metabolite correlation suggested that miraculin plays potential roles in regulating plant growth, seed germination and maturation, resisting pathogen infection, and environmental pressure. In summary, valuable genomic, transcriptomic, and metabolic resources provided in this study will promote the utilization of S. dulcificum and in-depth research on species in the Sapotaceae family.

Establishment of A Reproductive Health Care Carader in Integrated Service Post (Posyandu) Youth Village Penyengat Olak Muaro Jambi District

At the posyandu for youth, the IEC includes providing information about adolescent reproductive organs, puberty, the process of pregnancy, menstruation, family planning, sexually transmitted diseases, gender and maturity of marriage age. HIV / AIDS includes providing information about transmission, prevention and symptoms. Based on data from the Health Office of Muaro Jambi Regency, in the area of ??Puskesmas Penyengat Olak, the target number of adolescents aged 10-18 years is 1,959 males and 1,903 females. This large number is an opportunity to form a youth posyandu. Community service activities will be carried out in Penyengat Olak Village, Muaro Jambi Regency. This community service aims to increase the capacity of youth as cadres of the village post at Penyengat Olak district. Muaro Jambi. Target adolescents aged 10-18 years who are in the area of ??olak stinger health centers. There are activities in the form of advocacy to puskesmas and the community in establishing youth Posyandu, recruiting cadres and holding youth Posyandu cadres training, implementing youth Posyandu formation in stinging olak villages. The output of community service activities is the formation of cadres to care for reproductive health in pilot youth Posyandu for the work area of ??the Pengegat Olak Community Health Center in Muaro Jambi Regency. olak stinger. Through this training activity it is very important to increase the coverage of adolescent reproductive health services and it is hoped that the support of the puskesmas and village officials is expected.

Endometriosis’s impact on reproductive structures and its treatment

Endometriosis is a painful, chronic disease affecting approximately 10% of all women at reproductive age, and from the most common locations where endometriosis is found: the ovary, fallopian tubes, and uterine ligaments, and, less commonly they are found in the lung, the bladder, rectum, and intestines. It is known that endometriosis, in the reproductive organs, causes pelvic pain before or during menstruation, difficulty in pregnancy or infertility, for reasons that are not entirely clear, but some theories have tried to explain this phenomenon and confirmed that it may be a result of a defect in the immune and hormonal system in addition to some factors that affect egg’s quality and the movement of the gametes and therefore reproduction function. Treatment options now focus on pain management and attempting to limit the progression of implants, using a range of hormonal medications such as anti-progesterone, progestin, oral contraceptives, and GnRH, also including surgical procedures such as endometriosis and adhesion tissue. In this review, weshed light on the damage and issues that women may face due to this disease. We discuss some alternative solutions used by specialists for patient susceptibility.