Detection of bovine respiratory syncytial virus, Pasteurella multocida, and Mannheimia haemolytica by immunohistochemical method in naturally-infected cattle

AbstractIntroduction: The aim of this study was to determine the predisposing effect of bovine respiratory syncytial virus (BRSV) on Pasteurella spp. infection in naturally-induced pneumonia in cattle by immunohistochemical labelling.Material and Methods: Lungs of cattle slaughtered in the slaughterhouse were examined macroscopically, and 100 pneumonic samples were taken. The samples were fixed in 10% neutral formalin and embedded in paraffin by routine methods. Sections 5 μm in thickness were cut. The streptavidin-peroxidase method (ABC) was used to stain the sections for immuno-histochemical examination.Results: BRSV antigens were found in the cytoplasm of epithelial cells of bronchi, bronchioles, and alveoles and within inflammatory cell debris and inflammatory exudate in bronchial lumens. Pasteurella spp. antigens were detected in the cytoplasm of the epithelial cells of bronchi and bronchioles, and in cells in the lumens of bronchi and bronchioles. Eleven cases were positive for only one pathogen (six for BRSV and five for Pasteurella spp.), while 35 cases were positive for 2 pathogens: BRSV plus P. multocida (n = 21) or M. haemolytica (n = 14).Conclusion: The presence of high levels of BRSV in dual infections indicates that BSRV may be the main pneumonia-inducing agent and an important predisposing factor for the formation of Pasteurella spp. infections in cattle naturally afflicted with pneumonia.

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Bovine respiratory syncytial virus infection enhances Pasteurella multocida adherence on respiratory epithelial cells

Veterinary Microbiology ◽

10.1016/j.vetmic.2018.04.031 ◽

2018 ◽

Vol 220 ◽

pp. 33-38 ◽

Cited By ~ 11

Author(s):

Putu Eka Sudaryatma ◽

Kimika Nakamura ◽

Hirohisa Mekata ◽

Satoshi Sekiguchi ◽

Meiko Kubo ◽

...

Keyword(s):

Respiratory Syncytial Virus ◽

Epithelial Cells ◽

Virus Infection ◽

Respiratory Syncytial Virus Infection ◽

Pasteurella Multocida ◽

Bovine Respiratory Syncytial Virus ◽

Respiratory Epithelial Cells ◽

Syncytial Virus ◽

Respiratory Epithelial

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Bovine Respiratory Syncytial Virus Enhances the Adherence of Pasteurella multocida to Bovine Lower Respiratory Tract Epithelial Cells by Upregulating the Platelet-Activating Factor Receptor

Frontiers in Microbiology ◽

10.3389/fmicb.2020.01676 ◽

2020 ◽

Vol 11 ◽

Author(s):

Putu Eka Sudaryatma ◽

Akatsuki Saito ◽

Hirohisa Mekata ◽

Meiko Kubo ◽

Watcharapong Fahkrajang ◽

...

Keyword(s):

Respiratory Syncytial Virus ◽

Epithelial Cells ◽

Respiratory Tract ◽

Lower Respiratory Tract ◽

Pasteurella Multocida ◽

Platelet Activating Factor ◽

Bovine Respiratory Syncytial Virus ◽

Platelet Activating Factor Receptor ◽

Syncytial Virus

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The Role Of Primary Bovine Bronchial Epithelial Cells (BBEC) In Immune Regulation Regarding Response To Bovine Respiratory Syncytial Virus (BRSV) Infection

10.1164/ajrccm-conference.2011.183.1_meetingabstracts.a2096 ◽

2011 ◽

Author(s):

Thomas M. Krunkosky ◽

B. Heins ◽

J. Kyser ◽

L. Berghaus ◽

K. Galland ◽

...

Keyword(s):

Respiratory Syncytial Virus ◽

Epithelial Cells ◽

Immune Regulation ◽

Bronchial Epithelial Cells ◽

Bovine Respiratory Syncytial Virus ◽

Bronchial Epithelial ◽

Syncytial Virus

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Sites of Replication of Bovine Respiratory Syncytial Virus in Naturally Infected Calves as Determined by In Situ Hybridization

Veterinary Pathology ◽

10.1177/030098589603300403 ◽

1996 ◽

Vol 33 (4) ◽

pp. 383-390 ◽

Cited By ~ 31

Author(s):

B. Viuff ◽

Å. Uttenthal ◽

C. Tegtmeier ◽

S. Alexandersen

Keyword(s):

In Situ Hybridization ◽

Respiratory Syncytial Virus ◽

Epithelial Cells ◽

Respiratory Tract ◽

Bovine Respiratory Syncytial Virus ◽

The Other ◽

Alveolar Cells ◽

Rna Probes ◽

Syncytial Virus

Replication of bovine respiratory syncytial virus (BRSV) was studied in three naturally infected calves by in situ hybridization using strand-specific RNA probes. One of the calves was a 5-month-old Friesian, the other two calves were a 3-month-old and a 3-week-old Jersey. Two Jersey calves, 3 months and 3 weeks of age, served as controls. Replication of BRSV took place in the luminal lining of the respiratory tract. In one of the BRSV infected animals (calf No. 1), replication was especially seen in the bronchi, whereas in the two other animals (calf Nos. 2 and 3) replication of BRSV was demonstrated in the bronchiolar epithelial cells and in alveolar cells. Syncytia were often observed in the bronchiolar walls and in alveoli and such syncytia were always replicating BRSV. By immunohistochemistry it was possible to demonstrate BRSV antigen at the same location as replication of BRSV was detected. In tissue outside the respiratory tract neither BRSV antigen nor replication of BRSV could be demonstrated.

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A retrospective search for bovine respiratory syncytial virus (BRSV) antigens in histological specimens by immunofluorescence and immunohistochemistry

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x2000000400002 ◽

2000 ◽

Vol 20 (4) ◽

pp. 139-143 ◽

Cited By ~ 10

Author(s):

Eduardo F. Flores ◽

Rudi Weiblen ◽

Margareti Medeiros ◽

Sônia A. Botton ◽

Luiz F. Irigoyen ◽

...

Keyword(s):

Respiratory Syncytial Virus ◽

Epithelial Cells ◽

Giant Cells ◽

Bovine Respiratory Syncytial Virus ◽

Respiratory Pathogen ◽

Positive Staining ◽

Fixed Tissue ◽

Syncytial Virus ◽

Tissue Specimens ◽

Mononuclear Cell Infiltrates

Bovine respiratory syncytial virus (BRSV) has been only sporadically identified as a causative agent of respiratory disease in Brazil. This contrasts with frequent reports of clinical and histopathological findings suggestive of BRSV-associated disease. In order to examine a possible involvement of BRSV in cases of calf pneumonia, a retrospective search was performed for BRSV antigens in histological specimens submitted to veterinary diagnostic services from the states of Rio Grande do Sul and Minas Gerais. Ten out of 41 cases examined (24.4%) were positive for BRSV antigens by immunohistochemistry (IPX). Eight of these cases (19.5%) were also positive by indirect immunofluorescence (IFA), and 31 cases (75.6%) were negative in both assays. In the lungs, BRSV antigens were predominantly observed in epithelial cells of bronchioles and less frequently found in alveoli. In one case, antigens were detected only in the epithelium of the alveolar septae. The presence of antigen-positive cells was largely restricted to epithelial cells of these airways. In two cases, positive staining was also observed in cells and cellular debris in the exudate within the pulmonary airways. The clinical cases positive for BRSV antigens were observed mainly in young animals (2 to 12 month-old) from dairy herds. The main microscopic changes included bronchointerstitial pneumonia characterized by thickening of alveolar septae adjacent to airways by mononuclear cell infiltrates, and the presence of alveolar syncytial giant cells. In summary, the results demonstrate the suitability of the immunodetection of viral antigens in routinely fixed tissue specimens as a diagnostic tool for BRSV infection. Moreover, the findings provide further evidence of the importance of BRSV as a respiratory pathogen of young cattle in southeastern and southern Brazil.

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