scholarly journals Coupling of planar chromatography with Direct Analysis in Real Time mass spectrometry

2012 ◽  
Vol 10 (3) ◽  
pp. 703-710 ◽  
Author(s):  
Gertrud Morlock ◽  
Elena Chernetsova
Keyword(s):  
Mass Spectrometry ◽  
Real Time ◽  
Gas Flow ◽  
Direct Analysis ◽  
Ion Source ◽  
Ionization Mass ◽  
Planar Chromatography ◽  
Ionization Source ◽  
Desorption Ionization ◽  
Developing Area

AbstractDirect Analysis in Real Time mass spectrometry (DART-MS) is an emerging and rapidly developing area of ambient desorption ionization mass spectrometric techniques. Its coupling with planar chromatography is especially promising, as compared to other ambient desorption ionization techniques, because it does not require the use of liquids that may distort the shape of a spot by diffusion effects. In the first publications on TLC/HPTLC-DART-MS, due to the fixed, horizontally aligned supply of the gas flow from the DART ionization source to the MS inlet, the introduction of HPTLC/TLC plates as cut strips was inconvenient for quantitation, and the repeatability was very low due to the manual positioning. Recently a new version of the DART ion source was suggested, which allows adjusting the angle of the DART gas stream and the use of a motorized rail, thereby, improving highly the capabilities of TLC/HPTLC-DART-MS. This comprehensive review describes the development and analytical capabilities of TLC/HPTLC-DART-MS, and the general DART-MS perspectives for surface analysis or imaging MS.

Gas-Phase Reactivity of Acylphenols in Electrospray and Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

2009 ◽  
Vol 15 (2) ◽  
pp. 221-230 ◽  
Author(s):  
Isabelle Schmitz-Afonso ◽  
Vincent Guérineau ◽  
Alessandra Maia-Grondard ◽  
Khalijah Awang ◽  
Marc Litaudon ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Resolution Mass Spectrometry ◽  
Laser Desorption ◽  
Ion Source ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Acylphenols from Myristica crassa were identified based on liquid chromatography high-resolution mass spectrometry and liquid chromatography tandem mass spectrometry (MS/MS) experiments. Two types of compound were found in the extract of the plant: monomeric (malabaricone B and C) and dimeric compounds (C–C bonded biphenyl and C–C bonded phenyl–linear carbon chain). Evidence of formation of covalent dimeric ions during the electrospray ionization and matrix-assisted laser desorption ionization (MALDI) processes was established. [2M–3H]− dimeric ions were detected on the mass spectra of each monomeric compound during high-performance liquid chromatography separation. The MS/MS spectra of those species were compared to the MS/MS spectra obtained for the dimeric compounds synthesized by the plant. Fragmentation pathways were studied for the two classes of dimer. The dimeric ions formed in the ion source were C–C bonded biphenyl compounds. Further evidence was obtained from MALDI experiments: increase in the extraction delay time leads to an increase of the dimeric ions relative abundance. Their formation is based on the high reactivity of phenols or phenolate ions which are easily oxidized yielding phenoxy radicals.

scholarly journals Easily fabricated ion source for characterizing mixtures of organic compounds by direct analysis in real time mass spectrometry

2017 ◽  
Vol 9 (34) ◽  
pp. 5065-5074 ◽  
Author(s):  
Kathleen T. Upton ◽  
Katherine A. Schilling ◽  
J. L. Beauchamp
Keyword(s):  
Mass Spectrometry ◽  
Real Time ◽  
Organic Compounds ◽  
Direct Analysis ◽  
Ion Source ◽  
Ambient Mass Spectrometry ◽  
Assembly Cost

The EZ-DART ion source for ambient mass spectrometry, with simplified construction, minimal assembly cost, and versatile sampling capabilities, is described.

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