Transmission of Elegant Laguerre-Gaussian beams at a dielectric interface - numerical simulations

2009 ◽  
Vol 57 (2) ◽  
pp. 181-188 ◽  
Author(s):  
W. Szabelak ◽  
W. Nasalski
Keyword(s):  
Numerical Simulations ◽  
Total Internal Reflection ◽  
Polarization Effect ◽  
Internal Reflection ◽  
Gaussian Beams ◽  
Cross Polarization ◽  
Dielectric Interface ◽  
Theoretical Predictions ◽  
Critical Incidence

Transmission of Elegant Laguerre-Gaussian beams at a dielectric interface - numerical simulations Behaviour of Laguerre-Gaussian beams impinged at a dielectric interface under distinct angles is discussed. For different incident angles the beams interact with the interface differently. Two ranges of incident angles, specified by a position of a spectral cone of beam field and related to a cross-polarization effect, are analyzed. Boundary between these two ranges is defined. Cases of critical incidence and total internal reflection are also discussed. Paraxial beams near the lower paraxial limit are considered. Theoretical predictions are confirmed by numerical simulations.

General electromagnetic theory of total internal reflection fluorescence: the quantitative basis for mapping cell-substratum topography

1987 ◽  
Vol 87 (5) ◽  
pp. 677-693
Author(s):  
D. Gingell ◽  
O.S. Heavens ◽  
J.S. Mellor
Keyword(s):  
Glass Surface ◽  
Total Internal Reflection ◽  
Initial Step ◽  
Electromagnetic Theory ◽  
Internal Reflection ◽  
Total Internal Reflection Fluorescence ◽  
Water Soluble ◽  
Theoretical Predictions ◽  
Quantitative Basis ◽  
Aqueous Volume

Total internal reflection fluorescence (TIRF) has recently been used to look at the contacts made between cells and a glass surface on which they are spread. Our method utilizes the fluorescence of a water-soluble dye that acts as an extracellular aqueous volume marker. Fluorescence is stimulated by the short-range electric field near the glass surface that exists under conditions of total internal reflection. Since fluorescence is normally generated beneath a spread cell and not beyond it, the fluorescence of the image is related to the size of the cell-glass water gap. The images obtained are remarkable for their detail, contrast and the absence of confusing granularity due to cytoplasmic heterogeneity, which is commonly seen in interference reflection (IRM) images. We here develop a rigorous electromagnetic theory of total internal reflection in layered structures appropriate for cell contacts and apply it to quantitative TIRF. We show that: (1) TIRF, unlike IRM, can report cell-glass gaps in a way that is practically independent of the detailed physical properties of the cell; (2) TIRF is also far more sensitive than IRM for measuring cell-glass water gaps up to approximately equal to 100nm. These striking results explain the image quality seen by TIRF. As the initial step towards verifying our theory we show that measurement of the fluorescence stimulated by total internal reflection at a simple glass-water interface matches theoretical predictions.

Comparison of sensitivity of the refractometric methods of frustrated total internal reflection and surface plasmon resonance

2020 ◽  
pp. 44-49
Author(s):  
I. N. Pavlov
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Total Internal Reflection ◽  
Resonance Method ◽  
Internal Reflection ◽  
Optical Methods ◽  
Thin Boundary Layer ◽  
Experimental Conditions ◽  
Frustrated Total Internal Reflection

Two optical methods, namely surface plasmon resonance imaging and frustrated total internal reflection, are described in the paper in terms of comparing their sensitivity to change of refractive index of a thin boundary layer of an investigated medium. It is shown that, despite the fact that the theoretically calculated sensitivity is higher for the frustrated total internal reflection method, and the fact that usually in practice the surface plasmon resonance method, on the contrary, is considered more sensitive, under the same experimental conditions both methods show a similar result.

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