KERANGKA MANUSIA DARI SITUS GUA JAUHARLIN 1, KOTA BARU, KALIMANTAN SELATAN [THE HUMAN SKELETON FROM GUA JAUHARLIN 1, KOTA BARU, KALIMANTAN SELATAN]

Penelitian di situs Gua Jauharlin 1 telah dilakukan selama dua tahun, pada 2018 dan 2019. Pada tahun kedua diperoleh temuan kerangka manusia. Kondisinya hampir lengkap, tanpa bagian kaki, dan diberi kode GJL 1.1. Akan tetapi, di dekat cranium GJL 1.1, ditemukan sepasang tulang kaki manusia yang diduga milik individu GJL 1.1. Tujuan penelitian ini adalah menentukan identitas rangka GJL 1.1 berkaitan dengan data individu dan analisis konteks kuburnya. Penelitian ini menggunakan metode analisis makroskopis untuk data individu GJL 1.1, serta pendekatan arkeotanatologi untuk analisis konteks kuburnya. Analisis makroskopis menghasilkan informasi profil biologis GJL 1.11, yang mengindikasikan individu berjenis kelamin laki-laki, umur 26,9-42,5 tahun, tinggi badan 155,1 cm–165 cm, dan memiliki afiliasi dengan populasi Asia. Aktivitas mengunyah sirih pinang terindikasi berdasarkan fitur warna kuning kecoklatan pada permukaan labial dan buccal gigi individu GJL 1.1. Hasil analisis arkeotanatologi menunjukkan arsitektur kubur peletakan-penimbunan mayat GJL 1.1, serta tipe kubur yang bersifat primer. Hasil uji short tandem repeat combined deoxyribonucleic acid index system (STR CODIS) dengan menggunakan sampel dari sepasang tulang kaki dan rangka GJL 1.1, menunjukkan bahwa keduanya adalah individu yang berbeda. The two-season researches in Gua Jauharlin 1 site were carried out in 2018 and 2019. A human skeleton, sans its lower limbs, was discovered during the second season of excavation and coded GJL 1.1. However, a pair of human leg bones were found close to the cranium of GJL 1.1, which was suggested to belong to the individual of GJL 1.1. The research objective was to determine the identity of the GJL 1.1 in association with its individual attribute and the analysis of its burial context. This study uses a macroscopic analysis method to obtain individual data of GJL 1.1, as well as an archeothanatology approach to analyse the burial context. The macroscopic analysis yielded information on the biological profile of GJL 1.11 suggesting the individual is male, aged 26.9-42.5 years, height 155.1-165 cm, and has an affiliation with the Asian population. The brownish-yellow stain on the labial and buccal surface of human teeth of GJL 1.1 indicate betel nut chewing. The result of archeothanatological analysis suggests the architecture of the burial of GJL 1.1 with regard to laying-covering corpses and a primary burial. The results of the short tandem repeat combined deoxyribonucleic acid index system (STR CODIS) test, using samples from a pair of leg bones and the GJL 1.1 skeleton, indicate that the two came from different individuals.

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Various Defuzzification Methods on DNA Similarity Matching Using Fuzzy Inference System

Journal of Advanced Computational Intelligence and Intelligent Informatics ◽

10.20965/jaciii.2010.p0247 ◽

2010 ◽

Vol 14 (3) ◽

pp. 247-255

Author(s):

M. Rahmat Widyanto ◽

◽

Nurtami Soedarsono ◽

Norihiro Katayama ◽

Mitsuyuki Nakao ◽

...

Keyword(s):

Tandem Repeat ◽

Short Tandem Repeat ◽

Fuzzy Inference System ◽

Deoxyribonucleic Acid ◽

Fuzzy Inference ◽

Profile Data ◽

Similarity Matching ◽

Inference System ◽

Dna Profile ◽

Short Tandem

A DNA similarity matching using fuzzy inference system is proposed to measure a similarity between human STR (Short Tandem Repeat) based DNA (Deoxyribonucleic Acid) profiles. Moreover, various defuzzification methods are also tested to observe their behavior on different DNA data characteristics. Experiment on real human STR based DNA profile data shows that the proposed DNA similarity matching produces more realistic similarity values compared to those of the conventional one. Experiment on various defuzzification methods on DNA similarity matching shows that Sugeno defuzzification method is more suitable than those of other defuzzification methods.

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The use of touch DNA analysis in forensic identification focusing on Short Tandem Repeat-Combined DNA Index System loci THO1, CSF1PO and TPOX

Infectious Disease Reports ◽

10.4081/idr.2020.8716 ◽

2020 ◽

Author(s):

Ahmad Yudianto ◽

Indah Nuraini M. ◽

Abdul Hadi Furqoni ◽

Simon Martin Manyanza Nzilibili ◽

Pudji Harjanto

Keyword(s):

Tandem Repeat ◽

Short Tandem Repeat ◽

Index System ◽

Dna Analysis ◽

Forensic Identification ◽

Buccal Swab ◽

Dna Index ◽

Touch Dna ◽

Composite Gel ◽

Short Tandem

Forensic identification through DNA analysis is an accurate diagnostic tool. Deoxyribonucleic Acid (DNA) analysis is via DNA repetitive regions with less than 1 kb base size is called ‘microsatellite’ or Short Tandem Repeat (STR). At the crime scene, the perpetrator’s skin may accidentally be in contact with surrounding objects, thereby transferring trace evidence to the objects. In this study DNA was obtained using “touch DNA” from two buccal smears and two smear from watches and cellphones from volunteers who had signed the consent form. Samples were isolated using DNAzol. The quantity of DNA obtained will be measured using a UV spectrophotometer. For DNA amplification using 3 STR CODIS loci namely TH01, CSF1PO, and TPOX. The last step is visualization using acrylamide gel and silver staining. Mean levels of DNA (UVVisible Spectrophotometer) were 167.89±85.71 μg/mL for the buccal swab, 59.19±5.58 μg/mL for the watch swab, and 38.09±2.12 μg/mL for the mobile swab; the purity of the buccal swab DNA was 1.79±0.71, of the watch swab 1.69±0.76, and of the mobile swab 1.53±0.56. Visualization of PCR products on Polyacrylamide Agarose Composite Gel Electrophoresis stained with Silver and amplified using the standard primers THOI, TPOX and CSF1PO for STR Combined DNA Index System (CODIS) showed a 100% detection of amplicons. Both the buccal swab, watch swab and handphone swabs had trace amount of DNA that was sufficient to be isolated and amplified by using Polymerase Chain Reaction on the STR CODIS loci THO1, CSF1PO and TPOX.

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