scholarly journals A Study on Respiratory Control of Mitochondrial Preparation in Dog Gingiva

1986 ◽  
Vol 40 (1) ◽  
pp. 216-228
Author(s):  
Yoshiaki Haraga
Keyword(s):  
Respiratory Control ◽  
Mitochondrial Preparation

scholarly journals Preparation of rat enterocyte mitochondria

1984 ◽  
Vol 218 (2) ◽  
pp. 441-447 ◽  
Author(s):  
B Masola ◽  
D F Evered
Keyword(s):  
Cytochrome C ◽  
Oxidative Phosphorylation ◽  
Incubation Medium ◽  
Respiratory Control ◽  
Mitochondrial Fraction ◽  
Marker Enzymes ◽  
Cell Organelles ◽  
Succinate Dehydrogenase Activity ◽  
Isolation Medium ◽  
Mitochondrial Preparation

Rat enterocyte mitochondria were prepared with respiratory control ratios of 4 or 5 and occasionally 6. When EGTA was excluded from the mitochondrial incubation medium the calculated P/O ratios were high, especially those based on the first addition of ADP. These ratios were lowered by increasing the EGTA concentration from 1 mM to 2 mM in the mitochondrial preparation medium and including 1 mM-EGTA in the incubation medium. The use of EDTA in the enterocyte isolation medium led to the mitochondria requiring added cytochrome c. Substituting EGTA for EDTA abolished this requirement. The mitochondrial fraction consisted of two components, an upper cream-coloured layer rich in DNA and a lower brown-coloured layer poor in DNA. Both components were capable of oxidative phosphorylation with succinate or the glutamate/malate couple as substrates. The mitochondrial yield was assessed by assaying succinate dehydrogenase activity, and the contamination of the mitochondrial fraction by other cell organelles was assessed by assays for appropriate marker enzymes.

Oxygenation of 18-hydroxycorticosterone as the final reaction for aldosterone biosynthesis

1984 ◽  
Vol 107 (3) ◽  
pp. 395-400 ◽  
Author(s):  
Itaru Kojima ◽  
Etsuro Ogata ◽  
Hiroshi Inano ◽  
Bun-ichi Tamaoki
Keyword(s):  
Carbon Monoxide ◽  
Hydroxysteroid Dehydrogenase ◽  
Dependent Manner ◽  
In Vitro Production ◽  
Mitochondrial Preparation ◽  
Final Reaction ◽  
Vitro Production ◽  
Dose Dependent ◽  
Cytochrome P 450

Abstract. Incubation of 18-hydroxycorticosterone with the sonicated mitochondrial preparation of bovine adrenal glomerulosa tissue leads to the production of aldosterone, as measured by radioimmunoassay. The in vitro production of aldosterone from 18-hydroxycorticosterone requires both molecular oxygen and NADPH, and is inhibited by carbon monoxide. Cytochrome P-450 inhibitors such as metyrapone, SU 8000. SU 10603, SKF 525A, amphenone B and spironolactone decrease the biosynthesis of aldosterone from 18-hydroxycorticosterone. These results support the conclusion that the final reaction in aldosterone synthesis from 18-hydroxycorticosterone is catalyzed by an oxygenase, but not by 18-hydroxysteroid dehydrogenase. By the same preparation, the production of [3H]aldosterone but not [3H]18-hydroxycorticosterone from [1,2-3H ]corticosterone is decreased in a dose-dependent manner by addition of non-radioactive 18-hydroxycorticosterone.

Estimation of response slopes in respiratory control

1984 ◽  
Vol 56 (2) ◽  
pp. 536-539 ◽  
Author(s):  
D. L. Sherrill ◽  
G. D. Swanson
Keyword(s):  
Statistical Approach ◽  
Ventilatory Response ◽  
Respiratory Control ◽  
Major Axis ◽  
Bivariate Normal Distribution ◽  
Directional Statistics ◽  
Control Behavior ◽  
Slope Estimate ◽  
Partial Pressure Of Co2 ◽  
Reduced Major Axis

The ventilatory response to changes in alveolar (arterial) CO2 is widely used as an index of respiratory control behavior. Methods for estimating these response slopes should incorporate the possibility that there may be errors in both the independent (partial pressure of CO2) and dependent (ventilation) variables. In a recent paper Daubenspeck and Ogden (J. Appl. Physiol. Respirat. Environ. Exercise Physiol. 45:823–829, 1978) have suggested problems inherent in the traditional technique of reduced major axis and have suggested a more contemporary technique of directional statistics. We have previously analyzed both techniques and developed a method to overcome the problems of reduced major axis and problems inherent in the use of directional statistics. Under the assumption of a bivariate normal distribution, we demonstrate that our slope estimate is similar to the maximum likelihood estimate proposed by Mardia et al. (J. Appl. Physiol.: Respirat. Environ. Exercise Physiol. 54: 309–313, 1983) for this problem. In addition, we demonstrate a bootstrap statistical approach when the distributions are not normally distributed. These concepts are illustrated using O2-CO2 interaction data.

scholarly journals Influence of mitochondrial content on the sensitivity of respiratory control.

1987 ◽  
Vol 262 (19) ◽  
pp. 9109-9114
Author(s):  
G A Dudley ◽  
P C Tullson ◽  
R L Terjung
Keyword(s):  
Respiratory Control ◽  
Mitochondrial Content

Respiratory Relief: A New and Rapid Method for the Treatment of Phobic States

1971 ◽  
Vol 119 (553) ◽  
pp. 635-637 ◽  
Author(s):  
A. Orwin
Keyword(s):  
Rapid Method ◽  
Prolonged Exposure ◽  
Respiratory Control ◽  
Short Paper

The treatment of phobic states by desensitization is tedious and not always completely successful (Marks, 1969). Recently newer methods claiming improvement in speed and efficacy have been introduced, such as flooding or implosion (for example, Boulougouris et al., 1971) and prolonged exposure (Watson et al., 1971). An approach based on respiratory control which seems to be as effective as other methods and even more rapid in action is described in this short paper.

scholarly journals The Rapid Restoration of Respiratory Control to Uncoupled Mitochondria

1966 ◽  
Vol 241 (16) ◽  
pp. 3708-3713
Author(s):  
Eugene C. Weinbach ◽  
Joel Garbus
Keyword(s):  
Respiratory Control ◽  
Rapid Restoration
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