scholarly journals Frequency of mutation, lethality and efficiency of ethyl methane sulphonate and sodium azide on foxtail millet (Setaria italica [L.] P. Beauv.)

2018 ◽  
Vol 2 ◽  
pp. 9
Author(s):  
A. E. Esson ◽  
M. I. Adebola ◽  
A. G. Yisa
Keyword(s):  
Sodium Azide ◽  
Mutation Frequency ◽  
Foxtail Millet ◽  
Setaria Italica ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Morphological Mutant ◽  
High Mutation Frequency ◽  
Methane Sulphonate ◽  
Effectiveness And Efficiency

This study was carried out to determine the frequency of lethality, Mutagenic frequency, effectiveness and efficiency induced by ethyl methane sulphonate (EMS) and sodium azide on foxtail millet. The seeds were treated with different concentrations of EMS and sodium azide. Number of morphological mutant were observed visually and recorded. Mutagenic frequency, effectiveness and efficiency were highest for EMS at 0.1% concentration (Mutagenic frequency 7.50% effectiveness 18.75% and efficiency 7.50%) and sodium azide at 0.2% concentration (Mutagenic frequency 7.00%, effectiveness 1.84% and efficiency 1%). Lower concentrations of EMS and sodium azide were more effective and efficient in inducing mutation with low biological damages and high mutation frequency. EMS was more effective and efficient in inducing variability in foxtail millet than sodium azide. Therefore, mutants foxtail millet with low biological damages and high mutation frequency can be developed at low concentration of EMS and sodium azide for breeding of foxtail millet.

Induction and harnessing of polygenic variability in lentil (Lens culinaris Medik.)

2016 ◽  
Vol 39 (2) ◽  
Author(s):  
I. S. Solanki ◽  
Aman Rana
Keyword(s):  
Gamma Rays ◽  
Sodium Azide ◽  
Coefficient Of Variation ◽  
Lens Culinaris ◽  
Ethyl Methane Sulphonate ◽  
Multiple Traits ◽  
Ethyl Methane ◽  
Methane Sulphonate ◽  
Polygenic Traits ◽  
Medium Dose

Three mutagenised populations each of gamma rays, sodium azide (SA) and ethyl methane sulphonate (EMS) showed increased amount of variability over control for different polygenictraits in both <italic>macrosperma</italic> (LH90-54) and <italic>microsperma</italic> (LH89-48) lentil cultivars in M<sub>2</sub> generation. Among the mutagens tested, EMS induced maximum variability for all the traits, followed by gamma rays and SA in both the cultivars. The highest frequencies of mutated [M<sub>2</sub> progenies with higher coefficient of variation (CV) than the corresponding highest CV in control for any of the traits] and promising progenies [M<sub>2</sub> mutated progenies where mean shifted in the desired direction] were induced by different mutagens in the order:EMS > gamma rays > SA in both the cultivars (LH90-54 > LH89-48). In LH 90-54, promising progenies with multiple traits were induced with the highest frequency by SA, followed by EMS and gamma rays, whereas in LH89-48, they were induced with the highest frequency by SA, followed by gamma rays and EMS. Invariably, the medium dose of mutagens (0.08%SA, 0.40% EMSand 10 kRgamma rays) induced maximum variability(CV, %), resulting in thehighest number of promising progenies for all the polygenic traits.

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