scholarly journals Chemical mutagens (Ethyl methane sulphonate and sodium azide) mediated morphological characters and biochemical variations in green gram (Vigna radiata L.)

2016 ◽  
Vol 11 (1) ◽  
pp. 16-21
Author(s):  
RICHA SHARMA ◽  
K. SHARATH KUMAR
Keyword(s):  
Sodium Azide ◽  
Vigna Radiata ◽  
Morphological Characters ◽  
Green Gram ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Chemical Mutagens ◽  
Methane Sulphonate

scholarly journals Increased sensitivity to cell killing and mutagenesis by chemical mutagens in thymidine-kinase-deficient subclones of a Friend murine leukaemia cell line

1982 ◽  
Vol 40 (2) ◽  
pp. 207-212 ◽  
Author(s):  
P. G. McKenna ◽  
A. A. Yasseen
Keyword(s):  
Thymidine Kinase ◽  
Wild Type ◽  
Ethyl Methane Sulphonate ◽  
Leukaemia Cell ◽  
Leukaemia Cell Line ◽  
Ethyl Methane ◽  
Chemical Mutagens ◽  
Methane Sulphonate ◽  
Increased Sensitivity ◽  
Unit Dose

SUMMARYWild-type Friend murine leukaemia (clone 707) cells and two thymidinekinase-deficient subclones, 707BUE and 707BUF, were compared for sensitivity to killing and mutagenesis by the chemical mutagens, ethyl methane sulphonate (EMS),N-methyl-N′-nitro-N-nitrosoguanidine (MNNG), mitomycin C (MMC), and methyl methane sulphonate (MMS). The two thymidine-kinase-deficient subclones were significantly more sensitive to killing by each of the four chemical mutagens than were wild-type cells. The increased sensitivity to killing by the four mutagens was also reflected in increased mutagenesis (per unit dose of mutagen) to 6-thioguanine resistance. In the light of these results, the significance of thymidine kinase in DNA repair and mutagenesis is discussed.

Induction and harnessing of polygenic variability in lentil (Lens culinaris Medik.)

2016 ◽  
Vol 39 (2) ◽  
Author(s):  
I. S. Solanki ◽  
Aman Rana
Keyword(s):  
Gamma Rays ◽  
Sodium Azide ◽  
Coefficient Of Variation ◽  
Lens Culinaris ◽  
Ethyl Methane Sulphonate ◽  
Multiple Traits ◽  
Ethyl Methane ◽  
Methane Sulphonate ◽  
Polygenic Traits ◽  
Medium Dose

Three mutagenised populations each of gamma rays, sodium azide (SA) and ethyl methane sulphonate (EMS) showed increased amount of variability over control for different polygenictraits in both <italic>macrosperma</italic> (LH90-54) and <italic>microsperma</italic> (LH89-48) lentil cultivars in M<sub>2</sub> generation. Among the mutagens tested, EMS induced maximum variability for all the traits, followed by gamma rays and SA in both the cultivars. The highest frequencies of mutated [M<sub>2</sub> progenies with higher coefficient of variation (CV) than the corresponding highest CV in control for any of the traits] and promising progenies [M<sub>2</sub> mutated progenies where mean shifted in the desired direction] were induced by different mutagens in the order:EMS > gamma rays > SA in both the cultivars (LH90-54 > LH89-48). In LH 90-54, promising progenies with multiple traits were induced with the highest frequency by SA, followed by EMS and gamma rays, whereas in LH89-48, they were induced with the highest frequency by SA, followed by gamma rays and EMS. Invariably, the medium dose of mutagens (0.08%SA, 0.40% EMSand 10 kRgamma rays) induced maximum variability(CV, %), resulting in thehighest number of promising progenies for all the polygenic traits.

scholarly journals Frequency of mutation, lethality and efficiency of ethyl methane sulphonate and sodium azide on foxtail millet (Setaria italica [L.] P. Beauv.)

2018 ◽  
Vol 2 ◽  
pp. 9
Author(s):  
A. E. Esson ◽  
M. I. Adebola ◽  
A. G. Yisa
Keyword(s):  
Sodium Azide ◽  
Mutation Frequency ◽  
Foxtail Millet ◽  
Setaria Italica ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Morphological Mutant ◽  
High Mutation Frequency ◽  
Methane Sulphonate ◽  
Effectiveness And Efficiency

This study was carried out to determine the frequency of lethality, Mutagenic frequency, effectiveness and efficiency induced by ethyl methane sulphonate (EMS) and sodium azide on foxtail millet. The seeds were treated with different concentrations of EMS and sodium azide. Number of morphological mutant were observed visually and recorded. Mutagenic frequency, effectiveness and efficiency were highest for EMS at 0.1% concentration (Mutagenic frequency 7.50% effectiveness 18.75% and efficiency 7.50%) and sodium azide at 0.2% concentration (Mutagenic frequency 7.00%, effectiveness 1.84% and efficiency 1%). Lower concentrations of EMS and sodium azide were more effective and efficient in inducing mutation with low biological damages and high mutation frequency. EMS was more effective and efficient in inducing variability in foxtail millet than sodium azide. Therefore, mutants foxtail millet with low biological damages and high mutation frequency can be developed at low concentration of EMS and sodium azide for breeding of foxtail millet.

scholarly journals Analysis of Genetic Parameters on Mutant Populations of Mungbean (Vigna radiata L.) after Ethyl Methane Sulphonate Treatment

2012 ◽  
Vol 4 (1) ◽  
pp. 137-143 ◽  
Author(s):  
Rajib ROYCHOWDHURY ◽  
Sudipta DATTA ◽  
Parineeta GUPTA ◽  
Jagatpati TAH
Keyword(s):  
Genetic Variability ◽  
Plant Height ◽  
Vigna Radiata ◽  
Genetic Parameters ◽  
Heritability Estimate ◽  
Pure Line ◽  
Genetic Advance ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Methane Sulphonate

In the present study, pure line seeds of mungbean (Vigna radiata L. Wilczek) were treated with four doses (0.2%, 0.4%, 0.6% and 0.8% as w/v) of ethyl methane sulphonate (EMS) to analyse the genetic variability and heritability for some prime agro-metrical traits in second mutant (M2) generation. The analysis of variance revealed highly significant differences among the studied characters. The phenotypic coefficient of variation (PCV) was higher than its genotypic counterpart (GCV) for all the studied traits. The highest GCV (0.537) for number of seeds per pods and highest PCV (0.635) for plant height were recorded in 0.4% EMS treatment. The lowest GCV (0.179) and PCV (0.214) were recorded in the control set for number of pods per plant. The highest broad sense heritability estimate (92.33%) was recorded on 0.4% EMS for plant height. The expected genetic advance was high (42.39%) in 0.4% dose for days to flowering. The lowest heritability (38.43) and genetic advance (5.37) were noticed in control for the total seed yield per plant. EMS at 0.4% and 0.6% concentration gave the maximum values of all the genetic parameters. The increased genetic variability in treated population for these traits has a high scope for selection and can be exploited for the further improvement of mungbean.

Evaluation of Yield Attributing Variants Developed Through Ethyl Methane Sulphonate in an Important Proteinaceous Crop-Vicia faba

2017 ◽  
Vol 9 (2) ◽  
pp. 20-27 ◽  
Author(s):  
Durre Shahwar ◽  
Mohammad Yunus Khali Ansari ◽  
Sana Chaudhary ◽  
Rumana Aslam
Keyword(s):  
Vicia Faba ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Methane Sulphonate

scholarly journals Screening for pod shattering in mutant population of mungbean (Vigna radiata (L.) Wilczek)

2017 ◽  
Vol 9 (3) ◽  
pp. 1787-1791 ◽  
Author(s):  
N. Vairam ◽  
S. Anandhi Lavanya ◽  
C. Vanniarajan
Keyword(s):  
Gamma Rays ◽  
Vigna Radiata ◽  
Visual Observation ◽  
Induced Mutations ◽  
Ethyl Methane Sulphonate ◽  
Pod Shattering ◽  
Indian Agriculture ◽  
Methane Sulphonate ◽  
Important Objective ◽  
Pod Dehiscence

Mungbean, (Vigna radiata (L.) Wilczek) occupies a unique position in Indian agriculture and has been grown under various agro-ecological conditions. It is cultivated in 1.61mha with production of 3.38MT and productivi-ty of 474kg/ha in India. Mungbean pods are thin and brittle when dry, so shattering is a major problem. The loss of seeds by pod dehiscence is one of the major reasons for low yield in mungbean; thus, reducing the frequency of pod dehiscence is an important objective in mungbean breeding. Induced mutations, have offered a single and short alternative to conventional breeding including isolation, screening, selection and testing generation after generation. In this study, variability was induced by gamma rays and Ethyl methane sulphonate (EMS) in two greengram geno-types viz., CO (Gg) 7 and NM 65. Screening for pod shattering was carried out in M2 and M3 populations of green-gram. The scoring for shattering was recorded at physiological maturity of the pod. The shattering percentage ranged from 14.56 (400 Gy) to 93.45 per cent (20 mM). A total of 100 shattering tolerant mutants were selected from field based on visual observation. These mutants were again scored under laboratory condition as per IITA method. A total of 12 mutants of CO (Gg) 7 and 10 mutants of NM 65 which were tolerant to pod shattering were identified in M2 generation and forwarded to M3 generation. These mutants were scored for pod shattering under laboratory con-dition and nine mutants viz., M26, M44, M46, M58, M70, M71, M84, M92 and M98 were found to be tolerant in M3 generation. This study on identification and screening of the mutants tolerant to pod shattering with high yielding potential will help to increase the production of the pods to a greater extent.

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