Induced mutagenesis in finger-millet (Eleusine coracana Gaertn.) with gamma-rays and ethyl methane sulphonate—II. Chlorophyll mutation frequency and spectrum☆

1979 ◽  
Vol 19 (3) ◽  
pp. 123-126 ◽  
Author(s):  
R ARADHYA ◽  
P MADHAVAMENON
Keyword(s):  
Gamma Rays ◽  
Mutation Frequency ◽  
Finger Millet ◽  
Eleusine Coracana ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Chlorophyll Mutation ◽  
Methane Sulphonate ◽  
Induced Mutagenesis

scholarly journals Induced mutagenesis in Chickpea (Cicer arietinum (L.) with special reference to the frequency and spectrum of chlorophyll mutations

2016 ◽  
Vol 1 (1) ◽  
pp. 49 ◽  
Author(s):  
S. Umavathi ◽  
L. Mullainathan
Keyword(s):  
Cicer Arietinum ◽  
Gamma Rays ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Cicer Arietinum L ◽  
Chlorophyll Mutations ◽  
Methane Sulphonate ◽  
Chlorophyll Mutants ◽  
Induced Mutagenesis ◽  
Different Doses

A relative study of frequency and spectrum of chlorophyll mutations induced by mutagens in M2 generation was made with chickpea (Cicer arietinum (L). Variety ‘CO-4’. The treatments include different doses/concentrations of Gamma rays (20, 30, 40, 50 and 60kR) and Ethyl Methane Sulphonate (10, 20, 30, 40 and 50 mM). From the study, the overall frequencies and spectrum of five types of induced chlorophyll mutants Viridis (0.55), Xantha (0.46), Chlorina (0.45), Albina (0.43) and Tigrina (0.35) were observed. The frequency of chlorophyll mutation was increased with increasing concentrations up to a level, beyond it declined in both the mutagens. And the chlorophyll frequency was found in the order of viridis > xantha >chlorina> Albina >tigrina. The chemical mutagen, EMS was found to be more effective in inducing chlorophyll mutations than gamma rays in Chick pea.

scholarly journals Efficacy EMS and DES on Mutagenesis and seedling characters of Eleusine coracana L. Gaertn.

1970 ◽  
pp. 09-11
Author(s):  
L. Mullainathan, K. Aviya
Keyword(s):  
Seed Germination ◽  
Finger Millet ◽  
Germination Percentage ◽  
Eleusine Coracana ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Biological Damage ◽  
Methane Sulphonate ◽  
Ld50 Value ◽  
Seedling Characters

This study was performed by exposing the seeds of Finger millet (Eleusine coracana L. Gaertn.) Var CO -13 to Ethyl Methane Sulphonate (EMS) and Diethyl sulphate (DES). The observations were made on seed germination and survivability in M1 generation. The study revealed that germination percentage and survivability were decreased by increasing concentration of the mutagens when compared to the control. The LD50 value was found in 30mM of EMS and 40mM of DES. So it was found that both mutagens at lower treatments have influenced less biological damage and could be suitable for inducing desirable mutations in Finger millet.

Quantitative variation induced by gamma rays, ethyl methane sulphonate, and hydrazine hydrate in mung bean (Phaseolus aureus Roxb.)

1984 ◽  
Vol 26 (4) ◽  
pp. 492-496 ◽  
Author(s):  
Irfan A. Khan
Keyword(s):  
Gamma Rays ◽  
Seed Weight ◽  
Genetic Advance ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Plant Yield ◽  
Mean Values ◽  
Methane Sulphonate ◽  
The Mean ◽  
100 Seed Weight

Induced variability was studied in the quantitative characters of mung bean (Phaseolus aureus Roxb.) after treatment with gamma rays, ethyl methane sulphonate (EMS), and hydrazine hydrate (HZ) in M1, M2, and M3 generations. The characters evaluated were seeds per pod, 100-seed weight, and total plant yield. The variability increased in almost all of the characters in the M1 generation. The mean number of seeds per pod and 100-seed weight decreased, whereas the plant yield did not show any particular trend. There was an increase in the mean values after gamma irradiation and EMS treatments in the M2 generation. However, HZ fails to show the same response. However, the mean values increased in the M3 generation. Estimates of heritability were higher for 100-seed weight, followed by plant yield, and then seeds per pod in the M3 generation. Coefficient of genotypic variation and genetic advance were high for the plant yield in M2 and M3 generations. In general, the genotypic coefficient of variation values, heritability, and genetic advance increased more in M3 as compared with M2, indicating that the significant gain could possibly be achieved through selection in M3 generation.Key words: quantitative, heritability, mutagenesis.

scholarly journals Morphology and Yield Parameters and Biochemical Analysis of Soybean (Glycine max (L.) Mrr.) Using Gamma Rays, EMS and DES Treatment

2015 ◽  
Vol 35 ◽  
pp. 50-58 ◽  
Author(s):  
P. Gopinath ◽  
P. Pavadai
Keyword(s):  
Glycine Max ◽  
Plant Height ◽  
Seed Yield ◽  
Gamma Rays ◽  
Fruit Size ◽  
Mean Value ◽  
Ethyl Methane Sulphonate ◽  
Ethyl Methane ◽  
Yield Parameters ◽  
Methane Sulphonate

Soybean (Glycine max (L.) Merr.) var. Co-1 seeds were treated with physical mutagen namely such as gamma rays and chemical mutagen namely such as Ethyl Methane Sulphonate (EMS) and Diethyl Sulphate (DES). Various concentrations of gamma rays 10, 20, 30, 40, 50 and 60 KR, EMS (0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 %) and DES (0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 %). The morphological parameters such as days to first flower, plant height, number of cluster per plant, number of pod per plan, seed yield per plant, number of branches per plant, number of leaves per plant, protein content and oil content increasing with increasing level of some doses gamma rays, Ethyl methane sulphonate and Diethyl sulphate treatment in M2 and M3 generation were carried out. A number of mutants were recorded in M2 and M3 generations for one or more traits viz., plant height, maturity, branching, pigmentation, fruit size and yield were isolated in M2 and M3 generation. Mean for various characters increased at mutagenic treatment than control. The yield parameters like plant height, number of cluster per plant, number of seeds per plant and seed yield per plant were recorded the moderated and high mean value in the 50 KR of gamma rays 0.5% of EMS and 0.4% of DES treated population with compared to control plants.

scholarly journals Induced Mutagenesis in Cicer arietinum

2014 ◽  
Vol 12 ◽  
pp. 1-4 ◽  
Author(s):  
L. Mullainathan ◽  
S. Umavathi
Keyword(s):  
Cicer Arietinum ◽  
Gamma Rays ◽  
Crop Improvement ◽  
Ethyl Methane Sulphonate ◽  
Chick Pea ◽  
Biological Damage ◽  
Methane Sulphonate ◽  
Dose Concentration ◽  
Induced Mutagenesis ◽  
Effectiveness And Efficiency

The traditional varieties of chick pea have low potentiality and restricted variability with respect to economic characters. Broadening the genetic base for crop improvement can be quickly achieved through induced mutagenesis. The present study was undertaken in order to comparing the effectiveness and efficiency of mutagens on Cicer arietinum. In this regard, Co – 4 variety of chick pea was subjected to different dose/concentration of Gamma rays (20, 30, 40, 50 and 60 kR) and Ethyl Methane Sulphonate (10, 20, 30, 40 and 50 mM) for inducing mutation. Mutagenic effectiveness and efficiency was calculated based on biological damage in M1 and chlorophyll mutations in M2. The results indicated that, mutagenic effectiveness increased with the increase in dose/concentration of mutagen. Intermediate treatments in general were found more efficient in causing less biological damage and inducing maximum amount of mutations. It shows that the chemical mutagens are more effective and efficient than physical mutagen for inducing mutation in Chick pea

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