scholarly journals DE NOVO REGENERATION IN CHICKPEA GENOTYPES (CICER ARIETINUM L.)

2017 ◽  
Vol 8 ◽  
pp. 16-18
Author(s):  
VIKRAM JAMBHALE, SHARAD PAWAR
Keyword(s):  
Plant Regeneration ◽  
De Novo ◽  
Mature Embryo ◽  
Multiple Shoot ◽  
Direct Regeneration ◽  
Regeneration Capacity ◽  
Cicer Arietinum L ◽  
Shoot Production ◽  
Embryo Axes ◽  
The Media

Direct regeneration from mature embryo axes was achieved without intervening of callus phase in four chickpea varieties on the Media MS and B5 supplemented with combination of BAP, NAA and Kinetin. Hundred percent regeneration capacity was exhibited by commercially grown Vijay and Vishal varieties. There was considerable variation in umber of multiple shoot production by different varieties. Profuse rooting was obtained on the medium containing 0.5 and 1.0 mg/l IBA. This protocol is optimized for complete plant regeneration of chickpea for genetic transformation.

Multiple Shoot Induction and Plant Regeneration from Embryo Axes of Six Cultivars of Gossypium hirsutum

2003 ◽  
Vol 46 (3) ◽  
pp. 433-436 ◽  
Author(s):  
A.K. Banerjee ◽  
D.C. Agrawal ◽  
S.M. Nalawade ◽  
S. Hazra ◽  
K.V. Krishnamurthy
Keyword(s):  
Plant Regeneration ◽  
Gossypium Hirsutum ◽  
Multiple Shoot ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Embryo Axes

Multiple shoot induction by benzyladenine and complete plant regeneration from seed explants of chickpea (Cicer arietinum L.)

1997 ◽  
Vol 16 (8) ◽  
pp. 565-571 ◽  
Author(s):  
R. Polisetty ◽  
V. Paul ◽  
J. J. Deveshwar ◽  
S. Khetarpal ◽  
K. Suresh ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Cicer Arietinum ◽  
Multiple Shoot ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Cicer Arietinum L ◽  
Complete Plant

Plant regeneration from mature embryo-derived callus of Australian rice (Oryza sativa L.) varieties

2000 ◽  
Vol 51 (2) ◽  
pp. 305 ◽  
Author(s):  
Diah Azria ◽  
Prem L. Bhalla
Keyword(s):  
Plant Regeneration ◽  
Oryza Sativa L ◽  
Callus Formation ◽  
Shoot Formation ◽  
Mature Embryo ◽  
Multiple Shoot ◽  
Rice Varieties ◽  
Mature Embryos ◽  
Shoot Initiation

In vitro plant regeneration from callus induced from embryos of mature seeds of 4 Australian varieties of rice was studied. Observations of callus induction on MS and N6 media indicated that MS medium supplemented with 0.5–2 mg/L of 2,4-D is suitable for callus formation from the varieties tested. Comparison of shoot initiation on medium containing BAP, BAP + NAA, and TDZ + NAA indicated that these varieties prefer BAP + NAA or TDZ + NAA in the shoot initiation medium. Partial desiccation, resulting in up to 20% loss of fresh weight of callus, significantly increased the regeneration frequency of the 4 rice varieties tested. The varieties showed varied response to number of shoots produced per callus. Regenerated shoots were rooted on plant growth regulator free medium. The plants regenerated were phenotypically normal and fertile. Our study showed that callus derived from mature embryos of these rice varieties are amenable to multiple shoot formation, and could be used for genetic transformation studies.

Direct somatic embryogenesis from mature embryo axes in chickpea (Cicer arietinum L.)

Plant Science ◽  
1994 ◽  
Vol 102 (2) ◽  
pp. 189-194 ◽  
Author(s):  
K. Suhasini ◽  
A.P. Sagare ◽  
K.V. Krishnamurthy
Keyword(s):  
Somatic Embryogenesis ◽  
Cicer Arietinum ◽  
Mature Embryo ◽  
Direct Somatic Embryogenesis ◽  
Cicer Arietinum L ◽  
Embryo Axes

Multiple shoot induction by benzyladenine and complete plant regeneration from seed explants of chickpea (Cicer arietinum L.)

1997 ◽  
Vol 16 (8) ◽  
pp. 565-571 ◽  
Author(s):  
R. Polisetty ◽  
V. Paul ◽  
J. J. Deveshwar ◽  
S. Khetarpal ◽  
K. Suresh ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Cicer Arietinum ◽  
Multiple Shoot ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Cicer Arietinum L ◽  
Complete Plant

Plant Regeneration from Excised Cotyledons of Mature Strawberry Achenes

HortScience ◽  
1990 ◽  
Vol 25 (5) ◽  
pp. 569-571 ◽  
Author(s):  
A. Raymond Miller ◽  
Craig K. Chandler
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Multiple Shoot ◽  
Naphthaleneacetic Acid ◽  
Developmental Studies ◽  
Shoot Buds ◽  
Cotyledon Explants ◽  
Multiple Shoot Buds ◽  
Rapid Regeneration ◽  
Dichlorophenoxy Acetic Acid

A protocol was developed for excising and culturing cotyledon explants from mature achenes of strawberry (Fragaria × ananassa Duch.). Cotyledon explants formed callus with multiple shoot buds on agar-solidified Murashige and Skoog media containing several combinations of hormones (1 μm 2,4-D; 10 μm 2,4-D; 1 μm BA + 1 μm 2,4-D; 1 μm BA + 10 μm 2,4-D; 5 μm BA; 5 μm BA + 1 μm 2,4-D; 5 μm BA + 10 μ m 2,4-D; 5 μ m BA + 5 μm NAA; 5 μ m BA + 15 μ m NAA). After three subcultures, only tissues maintained on the medium containing 5 μm BA + 5 μm NAA continued to form shoots. Tissues transferred to other media eventually died (1 μm 2,4-D; 1 μ m BA + 10 μ m 2,4-D; 5 μ m BA; 5 μ m BA + 1 μ m 2,4-D), became unorganized (1 μm BA + 1 μm 2,4-D; 5 μm BA + 10 μm 2,4-D; 5 μm BA + 15 μm NAA), or formed roots (10 μm 2,4-D). Whole plantlets were produced by transferring callus with buds to medium lacking hormones. The rapid regeneration of clonal plantlets from cotyledon explants may be useful for reducing variability in future developmental studies. Chemical names used: N-(phenylmethyl)-1H-purin-6-amine (BA); (2,4-dichlorophenoxy) acetic acid (2,4-D); and 1-naphthaleneacetic acid (NAA).

scholarly journals In vitro Propagation of Polygonum hydropiper L. from Shoot Tips

2010 ◽  
Vol 20 (1) ◽  
pp. 73-79 ◽  
Author(s):  
M. F. Hasan ◽  
B. Sikdar
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Root Induction ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Polygonum Hydropiper

An efficient protocol for plant regeneration through multiple shoots induction from shoot tips of Polygonum hydropiper (L.) was established. The highest percentage (96.6) of multiple shoot induction and number of shoots (9.0) per culture were found on MS supplemented with 2.0 mg/l Kn. The induced shoots were excised and inoculated on to MS contains different concentrations of IBA or NAA for rooting. The highest percentage (90.0) of root induction and the highest number of roots per shoot (12.0) was found on MS having 1.0 mg/l IBA. Well rooted plantlets were acclimated properly and transplanted in the soil under natural condition, where cent per cent plantlets survived and grew successfully. Key words:  Polygonum hydropiper, Shoot tips, In vitro propagation D.O.I. 10.3329/ptcb.v20i1.5970 Plant Tissue Cult. & Biotech. 20(1): 73-79, 2010 (June)

Agrobacterium mediated transformation of chickpea ( Cicer arietinum L.) embryo axes

2000 ◽  
Vol 19 (3) ◽  
pp. 235-240 ◽  
Author(s):  
K. V. Krishnamurthy ◽  
K. Suhasini ◽  
A. P. Sagare ◽  
M. Meixner ◽  
A. de Kathen ◽  
...  
Keyword(s):  
Cicer Arietinum ◽  
Agrobacterium Mediated Transformation ◽  
Cicer Arietinum L ◽  
Embryo Axes

scholarly journals Direct multiple shoot induction and plant regeneration from dormant buds of Codonopsis pilosula (Franch.) Nannf.

2011 ◽  
Vol 10 (51) ◽  
pp. 10509-10515 ◽  
Author(s):  
H Zhang Y ◽  
F Gao S ◽  
Du T ◽  
G Chen H ◽  
H Z Wang ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Multiple Shoot ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Codonopsis Pilosula

Successful plant regeneration system via de novo organogenesis in Syzygium cumini (L.) Skeels: an important medicinal tree

2018 ◽  
Vol 93 (4) ◽  
pp. 1285-1295 ◽  
Author(s):  
Afshan Naaz ◽  
Sheikh Altaf Hussain ◽  
Ruphi Naz ◽  
Mohammad Anis ◽  
Abdulrahman A. Alatar
Keyword(s):  
Plant Regeneration ◽  
De Novo ◽  
Syzygium Cumini ◽  
Regeneration System ◽  
Medicinal Tree
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