Successful plant regeneration system via de novo organogenesis in Syzygium cumini (L.) Skeels: an important medicinal tree

2018 ◽  
Vol 93 (4) ◽  
pp. 1285-1295 ◽  
Author(s):  
Afshan Naaz ◽  
Sheikh Altaf Hussain ◽  
Ruphi Naz ◽  
Mohammad Anis ◽  
Abdulrahman A. Alatar
Keyword(s):  
Plant Regeneration ◽  
De Novo ◽  
Syzygium Cumini ◽  
Regeneration System ◽  
Medicinal Tree

High frequency plant regeneration following abnormal shoot organogenesis in the medicinal tree Hovenia dulcis

2009 ◽  
Vol 98 (1) ◽  
pp. 59-65 ◽  
Author(s):  
Mi Jin Jeong ◽  
Hyun Jin Song ◽  
Dong Jin Park ◽  
Ji Yun Min ◽  
Jin Seong Jo ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
High Frequency ◽  
Shoot Organogenesis ◽  
Medicinal Tree ◽  
Hovenia Dulcis

An efficient plant regeneration system with in vitro flavonoid accumulation for Hylotelephium tatarinowii (Maxim.) H. Ohba

2010 ◽  
Vol 46 (5) ◽  
pp. 445-450 ◽  
Author(s):  
Junli Wang ◽  
Jue Wang ◽  
Kun Liu ◽  
Xuan Xiao ◽  
Weizhen Gong ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Regeneration System ◽  
Flavonoid Accumulation

scholarly journals In Vitro Regeneration Potential of White Lupin (Lupinus albus) from Cotyledonary Nodes

Plants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 318
Author(s):  
Mehtab Muhammad Aslam ◽  
Joseph K. Karanja ◽  
Qian Zhang ◽  
Huifeng Lin ◽  
Tianyu Xia ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Genetic Engineering ◽  
Shoot Regeneration ◽  
Lupinus Albus ◽  
Cotyledonary Node ◽  
White Lupin ◽  
Regeneration System ◽  
Regeneration Frequency ◽  
Cotyledonary Nodes

The tissue culture regeneration system of Lupinus albus has always been considered as recalcitrant material due to its genotype-dependent response and low regeneration efficiency that hamper the use of genetic engineering. Establishment of repeatable plant regeneration protocol is a prerequisite tool for successful application of genetic engineering. This aim of this study was to develop standardized, efficient protocol for successful shoot induction from cotyledonary node of white lupin. In this study, 5 day old aseptically cultured seedlings were used to prepare three explants (half cotyledonary node, HCN; whole cotyledonary node, WCN; and traditional cotyledonary node, TCN), cultured on four concentrations of M519 medium (M519, ½ M519, 1/3 M519, and ¼ M519), containing four carbohydrate sources (sucrose, fructose, maltose, and glucose), and stimulated with various combinations of KT (kinetin), and NAA (naphthalene acetic acid) for direct shoot regeneration. High frequency of 80% shoot regeneration was obtained on ½ M519 medium (KT 4.0 mg L−1 + NAA 0.1 mg L−1) by using HCN as an explant. Interestingly, combinations of (KT 4.0 mg L−1 + NAA 0.1 mg L−1 + BAP 1.67 mg L−1), and (KT 2.0 mg L−1 + NAA 0.1 mg L−1) showed similar shoot regeneration frequency of 60%. Augmentation of 0.25 g L−1 activated charcoal (AC) not only reduced browning effect but also improved shoot elongation. Among the all carbohydrate sources, sucrose showed the highest regeneration frequency with HCN. Additionally, 80% rooting frequency was recorded on ½ M519 containing IAA 1.0 mg L−1 + KT 0.1 mg L−1 (indole acetic acid) after 28 days of culturing. The present study describes establishment of an efficient and successful protocol for direct plant regeneration of white lupin from different cotyledonary nodes.

De novo shoot organogenesis during plant regeneration

2019 ◽  
Vol 71 (1) ◽  
pp. 63-72 ◽  
Author(s):  
Jinwoo Shin ◽  
Soonhyung Bae ◽  
Pil Joon Seo
Keyword(s):  
Cell Cycle ◽  
Plant Regeneration ◽  
Shoot Regeneration ◽  
Cell Cycle Progression ◽  
De Novo ◽  
Shoot Organogenesis ◽  
Regeneration Process ◽  
Cycle Progression ◽  
Ros Metabolism ◽  
Molecular Components

We reconstituted the shoot regeneration process in callus with four phases and also scrutinized molecular components involved in phytohormonal interactions, ROS metabolism, cell cycle progression. and bioelectrical signaling during shoot regeneration.

Establishment of an efficient plant regeneration system of dodder

2008 ◽  
Vol 136 ◽  
pp. S154-S155
Author(s):  
Dongxiao Li ◽  
Jingling Chang ◽  
Guoguang Zhang ◽  
Liang Chen
Keyword(s):  
Plant Regeneration ◽  
Regeneration System
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