scholarly journals Identification and Characterization of a Pseudomonas mosselii strain and its Antibacterial Function against Agrobacterium tumefaciens

2021 ◽  
Vol 4 (2) ◽  
pp. 223-231
Author(s):  
Zhilong Wang ◽  
◽  
Wen Li ◽  
Jiaying Wang ◽  
Xintong Jiang ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Biochemical Characterization ◽  
Inhibition Zone ◽  
Tumor Formation ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Cherry Tree ◽  
Bacterial Colony ◽  
Crown Gall Disease

Agrobacterium tumefaciens is a gram negative bacterial that can infect a range of plants and result in root crown gall. A total number of 10328 bacterial strains were isolated from rhizosphere of cherry tree. One strain of LWB10 showed clear inhibition zone around the bacterial colony in YEB media inoculated with A. tumefaciens C58. Morphological, physiological, and biochemical characterization indicated that LWB10 belongs to member of the genus Pseudomonas. Results from the high-throughput matrix-assisted laser desorption/ionization biotypersmart system indicated that this strain had a score value of 2.247 relative to Pseudomonas mosselii. Also, phylogenetic analysis based on 16S rRNA gene sequence showed that strain LWB10 shared the highest similarity with Pseudomonas mosselii CIP 105259T. The antagonist strains also exhibit well in growth inhibition of other five A. tumefaciens strains. Coinoculation of LWB10 and plant pathogenic strain of A. tumefaciens CFCC1369 showed strongly inhibition of tumor formation in tomato stems. All the results demonstrated that the isolated strain is P. mosselii LWB10 and its antibacterial ability to A. tumefaciens may offer new way for management of crown gall disease in the future. Keywords: Agrobacterium tumefaciens, Pseudomonas mosselii, antagonist, biological control

The effects of salicylates on phenomena related to crown gall

1984 ◽  
Vol 62 (4) ◽  
pp. 729-734 ◽  
Author(s):  
B. Saint-Pierre ◽  
L. Miville ◽  
P. Dion
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Mutant Strain ◽  
Crown Gall ◽  
Tumor Tissue ◽  
Tumor Formation ◽  
Crown Gall Disease ◽  
Low Concentrations ◽  
Rate Of Photosynthesis ◽  
Ph Dependent ◽  
High Concentrations

The effects of acetylsalicylate and salicylate were investigated on three phenomena involved in crown-gall disease. First, an inhibition of the growth of Agrobacterium tumefaciens was observed with low concentrations of salicylates. This inhibition was pH dependent and was partially reversed by glutamate. Second, an inhibition of the growth of tobacco tumor tissue was observed only at high concentrations of salicylates. Third, tumor formation on excised tobacco leaves was inhibited by low concentrations of salicylates. The inhibition of tumor formation occurred at salicylate concentrations that had no significant effect on the net rate of photosynthesis by the leaves. The inhibition of tumor formation was also observed with a mutant strain of A. tumefaciens resistant to salicylate. The potential interest of salicylates as control agents for crown-gall disease is discussed.

scholarly journals Antibacterial effect of Artemisia and ginger extracts in controlling Agrobacterium tumefaciens in roses

2021 ◽  
pp. 1-5
Author(s):  
Alfred Njagi ◽  
Methuselah Nyamwange Mang'erere ◽  
Ezekiel Mugendi Njeru ◽  
Jonah Kiprono Birgen
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Stem Length ◽  
Copper Hydroxide ◽  
Cut Flowers ◽  
Promising Alternative ◽  
Crown Gall Disease

Rose is the world’s most traded cut flowers with 74 % being produced in Kenya. Pests like spider mites, caterpillar, aphids, thrips, nematodes and diseases such as crown gall, downy mildew, powdery mildew and botrytis highly compromise rose production. Crown gall disease caused by Agrobacterium tumefaciens is the most problematic disease of roses in Kenya, causing a production loss of up to 60 % depending on the age and variety of rose. An experiment to determine the antibacterial effect of Artemisia and ginger extracts was carried out in vitro, where the inhibitory zones around filter discs soaked with extracts on Muller Hinton agar was established. The extracts were emulsified with dimethylsulfoxide (50 %) and minimum inhibitory concentrations of Artemisia (125 mg/ml), ginger (62.5 mg/ml) and mixture of Artemisia and ginger (31.25 mg/ml) were used to soak the filter discs whereas the commercial recommended rate of copper hydroxide of 6.25 mg/ml was used. Artemisia and copper hydroxide (commercial antibiotic) had highest inhibition zone of 12.80 mm compared to ginger 10.60 mm. A mixture of Artemisia and Ginger had a slightly lower inhibition zone (10.20 mm) though not significantly different from ginger (P> 0.001). An eight-month greenhouse experiment was also done to determine the efficacy of the extracts on inoculated rose plants. The results showed that crown gall incidence and gall weight were low but not significantly different from copper hydroxide and Artemisia. Crop vigor, which was indicated by stem length was highest for Artemisia treatment with an average of 73.54 cm followed by copper hydroxide (67.25 cm) while ginger and mixture of ginger and Artemisia had 53.44 cm and 64.70 cm respectively. From the results of this research, Artemisia and Ginger extracts are promising alternative to control crown gall and possibly other diseases in field crops. Artemisia performance compares well with copper hydroxide and therefore the best alternative to replace copper hydroxide.

Perception of Agrobacterium tumefaciens flagellin by FLS2XL confers resistance to crown gall disease

Nature Plants ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 22-27 ◽  
Author(s):  
Ursula Fürst ◽  
Yi Zeng ◽  
Markus Albert ◽  
Anna Kristina Witte ◽  
Judith Fliegmann ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Crown Gall Disease

Untersuchungen an Crown-Gall

1970 ◽  
Vol 25 (7) ◽  
pp. 735-738 ◽  
Author(s):  
Rolf Beiderbeck
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Tumor Formation ◽  
Tumor Induction ◽  
Host System ◽  
Induced Tumors ◽  
Kalanchoë Daigremontiana ◽  
Kalanchoe Daigremontiana

Concentrations of 10 µg/ml rifampicin reduce the growth of Agrobacterium tumefaciens by more than 90%, concentrations of 100 µg/ml prevent tumor formation in Kalanchoë daigremontiana by the same bacteria.A treatment with rifampicin does not irreversibly alter the bacteria; after removal of the drug bacterial propagation and the ability of tumor induction are restored.0-24 h treatments of the plant wounds with rifampicin prior to infection prevent tumor formation completely.Addition of rifampicin after infection inhibits tumor formation optimally if the drug is added only few hours after inoculation. 30 h after infection the bacteria-host-system is resistant against rifampicin.100 μg/ml rifampicin do not influence the growth of already induced tumors.

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