scholarly journals Optimizing the Duration of Acclimatization under Artificial Light for St. John's Wort Plantlets Grown Photoautotrophically and Photomixotrophically In Vitro

2006 ◽  
Vol 44 (1) ◽  
pp. 63-70 ◽  
Author(s):  
Marcio Akira COUCEIRO ◽  
Sayed M. A. ZOBAYED ◽  
Fawzia AFREEN ◽  
Eiji GOTO ◽  
Toyoki KOZAI
2002 ◽  
Vol 162 (2) ◽  
pp. 193-202 ◽  
Author(s):  
Veronika Butterweck ◽  
Adolf Nahrstedt ◽  
Jon Evans ◽  
Sandy Hufeisen ◽  
Laura Rauser ◽  
...  

2011 ◽  
Vol 72 (14-15) ◽  
pp. 1814-1820 ◽  
Author(s):  
Satomi Onoue ◽  
Yoshiki Seto ◽  
Masanori Ochi ◽  
Ryo Inoue ◽  
Hideyuki Ito ◽  
...  

HortScience ◽  
2002 ◽  
Vol 37 (6) ◽  
pp. 978-980 ◽  
Author(s):  
Joe-Ann McCoy ◽  
N.D. Camper

Hypericum perforatum L. (St. John's Wort) has an extensive history as an important medicinal herb used for the treatment of neurological and depressive disorders (Linde et al., 1996). The objective of this study was to establish an in vitro tissue culture protocol for St. John's Wort. Nodal segments, axillary buds, and leaf disc explants produced multiple shoots and callus on Murashige and Skoog minimal organics medium supplemented with combinations of indoleacetic acid (IAA; 0.57, 2.85, 5.71 μm) and benzylaminopurine (BA; 2.22, 4.44, 8.88 μm). Shoot production occurred on all combinations of IAA/BA tested and was significantly less in treatments without hormones. Callus production was higher on treatments containing 2.85 μm IAA + 4.44 μm BA, or 5.71 μm IAA + 8.88 μm BA. Shoots transferred to hormone-free medium at 8 weeks formed roots by 12 weeks. A micropropagation protocol was established for St. John's Wort using mature plants as the explant source.


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