In Vitro Platelet Aggregation Inhibition Activity of Psophocarpus tetragonolobus (L.) Dc Pod Extract

The study aimed to investigate antiplatelet activity of the pods of Psophocarpus tetragonolobus in vitro in search for a new botanical source of a platelet aggregation inhibitor for the prevention of stroke, the second leading cause of mortality in the Philippines. This study utilized maceration with hexane as the extraction method. Four concentrations (4mg/mL, 2mg/mL and 0.5mg/mL) of Psophocarpus tetragonolobus pods were used in the study. Aspirin (2mg/mL) and NSS were the positive and negative controls, respectively. The methods employed in the study were Giemsa Microplate Assay for qualitative analysis and UV-Vis spectrophotometric analysis for quantitative analysis. Giemsa microplate assay findings revealed that the 4.0mg/mL test solution had the least violet gels present to no violet gels visible at all. Furthermore, the UV-Vis Spectrophotometric Analysis corresponded with the results and found that the 4mg/mL concentration contained the least mean absorbance reading (among the pod extracts) of 0.409, the highest average percent platelet aggregation inhibition of 69.58% (± 2.93) and the greatest average percent antiplatelet activity of 75.49% (± 3.07), among the pod extracts. In conclusion, the pod extract, at 4.0mg/mL concentration, was able to inhibit platelet aggregation.

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A Physiologic Regulator of Collagen-Induced Platelet Aggregation: Inhibition by Clq

Thrombosis and Haemostasis ◽

10.1055/s-0038-1650145 ◽

1981 ◽

Vol 45 (02) ◽

pp. 110-115 ◽

Cited By ~ 5

Author(s):

György Csákó ◽

Eva A Suba

Keyword(s):

Platelet Aggregation ◽

Human Platelet ◽

Platelet Reactivity ◽

High Specificity ◽

Turbidimetric Method ◽

Specific Manner ◽

Aggregation Inhibition ◽

Different Tissues

SummaryPlatelet aggregations were studied by a turbidimetric method in citrated human platelet-rich plasmas (PRP) in vitro. Human Clq inhibited the aggregations caused by collagens derived from different tissues and species. Clq was needed by weight in comparable quantities to collagen for neutralizing the aggregating effect. The dependence of the inhibitory reaction on the preincubation of platelets with Clq and the differences in the occurrence of aggregating substances in supernatants of PRP triggered with collagen in the presence or absence of Clq, confirmed that Clq exerts its effect by preventing fixation of collagen to platelets. In addition, the high specificity of the inhibitory action of Clq for collagen-induced platelet aggregation was demonstrated by results obtained for testing a variety of aggregating agents in combination with Clq and/or collagen.Since normal concentrations of Clq in the blood are in the range of inhibitory doses of Clq for collagen-induced platelet aggregations in vitro and upon activation of complement Clq is known to dissociate from Cl, it is proposed that Clq may participate in a highly specific manner in regulating platelet reactivity to collagen in vivo.

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