The study aimed to investigate antiplatelet activity of the pods of Psophocarpus tetragonolobus in vitro in search for a new
botanical source of a platelet aggregation inhibitor for the prevention of stroke, the second leading cause of mortality in
the Philippines. This study utilized maceration with hexane as the extraction method. Four concentrations (4mg/mL,
2mg/mL and 0.5mg/mL) of Psophocarpus tetragonolobus pods were used in the study. Aspirin (2mg/mL) and NSS were
the positive and negative controls, respectively. The methods employed in the study were Giemsa Microplate Assay for
qualitative analysis and UV-Vis spectrophotometric analysis for quantitative analysis. Giemsa microplate assay findings
revealed that the 4.0mg/mL test solution had the least violet gels present to no violet gels visible at all. Furthermore, the
UV-Vis Spectrophotometric Analysis corresponded with the results and found that the 4mg/mL concentration contained
the least mean absorbance reading (among the pod extracts) of 0.409, the highest average percent platelet aggregation
inhibition of 69.58% (± 2.93) and the greatest average percent antiplatelet activity of 75.49% (± 3.07), among the pod
extracts. In conclusion, the pod extract, at 4.0mg/mL concentration, was able to inhibit platelet aggregation.