The Effect of Carbohydrates and Sulfur on B-Vitamins Synthesis, Cellulose Digestion, and Urea Utilization by Rumen Microorganisms In Vitro

1954 ◽  
Vol 13 (3) ◽  
pp. 570-580 ◽  
Author(s):  
Chas. H. Hunt ◽  
Orville G. Bentley ◽  
T. V. Hershberger ◽  
J. H. Cline
Keyword(s):  
B Vitamins ◽  
Rumen Microorganisms ◽  
Cellulose Digestion

scholarly journals Influence of Chlortetracycline on in Vitro Cellulose Digestion by Bovine Rumen Microorganisms

1956 ◽  
Vol 39 (3) ◽  
pp. 303-311 ◽  
Author(s):  
J.R. Lodge ◽  
J.T. Miles ◽  
N.L. Jacobson ◽  
L.Y. Quinn
Keyword(s):  
Rumen Microorganisms ◽  
Cellulose Digestion ◽  
Bovine Rumen

EFFECTS OF THIAMINE INHIBITORS ON CELLULOSE DIGESTION AND VOLATILE FATTY ACID PRODUCTION IN ARTIFICIAL RUMEN FERMENTATIONS

1965 ◽  
Vol 45 (2) ◽  
pp. 99-104 ◽  
Author(s):  
L. P. Milligan ◽  
J. M. Asplund ◽  
A. R. Robblee
Keyword(s):  
Fatty Acid ◽  
Acid Production ◽  
Volatile Fatty Acid ◽  
Fermentation Medium ◽  
Rumen Fermentation ◽  
Fatty Acid Production ◽  
Rumen Microorganisms ◽  
Cellulose Digestion ◽  
Structural Analogues

The effect of the inclusion of thiamine inhibitors in a rumen fermentation medium on cellulose digestion and volatile fatty acid production by rumen microorganisms in vitro was studied. The addition of the structural analogues oxythiamine and neopyrithiamine to the fermentation medium at concentrations up to 4000 μM and 2000 μM, respectively, did not influence cellulose digestion or volatile fatty acid production. The addition of thiopental and hexetidine to the fermentation medium affected both cellulose digestion and volatile fatty acid production. Some inhibition of cellulose digestion and of volatile fatty acid production occurred with concentrations of thiopental and hexetidine in the fermentation medium as low as 720 μM and 15 μM respectively.

scholarly journals The Effect of Antibiotics on in Vitro Cellulose Digestion by Rumen Microorganisms

1952 ◽  
Vol 35 (7) ◽  
pp. 571-580 ◽  
Author(s):  
R.H. Wasserman ◽  
C.W. Duncan ◽  
E.S. Churchill ◽  
C.F. Huffman
Keyword(s):  
Rumen Microorganisms ◽  
Cellulose Digestion

THE EFFECTS OF VARIATIONS IN VOLUME OF INOCULA ON THE IN VITRO CELLULOSE DIGESTION BY RUMEN MICROORGANISMS SUPPORTED BY NUTRITIONALLY INADEQUATE MEDIA

1964 ◽  
Vol 44 (1) ◽  
pp. 24-28 ◽  
Author(s):  
R. R. Marquardt ◽  
J. M. Asplund
Keyword(s):  
Nutrient Medium ◽  
Bacterial Population ◽  
Statistical Control ◽  
Rumen Microorganisms ◽  
Cellulose Digestion

The effects of increasing volumes of inocula on the rate of cellulose digestion supported by various nutritionally inadequate media were studied. Cellulose digestion increased as the volume of inoculum increased. A comparison of additions of fresh vs. autoclaved inocula indicated that this increase was largely due to a larger bacterial population. It is suggested that, unless the nutrient medium used in artificial rumen studies is completely adequate, standardization of inocula or statistical control is necessary.

THE EFFECTS OF WATER EXTRACTS OF FORAGES ON IN VITRO CELLULOSE DIGESTION BY RUMEN MICROORGANISMS

1964 ◽  
Vol 44 (1) ◽  
pp. 16-23 ◽  
Author(s):  
R. R. Marquardt ◽  
J. M. Asplund
Keyword(s):  
Nutrient Medium ◽  
Aqueous Extracts ◽  
Rumen Microorganisms ◽  
Cellulose Digestion ◽  
Adequate Supply ◽  
Water Extracts

Aqueous extracts of 14 forages of widely varying quality were buffered with Na2CO3 and used as media for growth of rumen microorganisms. The ability of these media, with and without supplementation with phosphate–sulphate and/or urea, to support cellulose digestion was studied. None of the unsupplemented extracts supported maximum cellulolysis. Although cellulose digestion was improved by supplementation, the extent of improvement by the addition of phosphate–sulphate vs. urea varied with the forage extract. Both supplements together increased cellulose digestion more than the sum of the increases when they were added separately and usually resulted in rates of cellulose digestion approaching that obtained with a complete nutrient medium. Considerable variation between inoculum groups was observed when forage extracts were used as media. It was concluded that, with the exception of phosphate, sulphate, and, in some cases, nitrogen, an adequate supply of the nutrients required for cellulose digestion by rumen microorganisms was usually present in the aqueous extracts of forages.

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