scholarly journals STUDI PERBANDINGAN PEMAKAIAN PRIMER HBS1 DAN HBS2 DENGAN PRIMER P7 DAN P8 DALAM DETEKSI DNA VIRUS HEPATITIS B PADA PENDONOR DARAH

2020 ◽  
Vol 7 (2) ◽  
pp. 73
Author(s):  
Supiana Dian Nurtjahyani ◽  
Mohammad Amin ◽  
Retno Handajan
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Blood Donors ◽  
Hbv Dna ◽  
Chain Reaction ◽  
Hepatitis B Virus Dna ◽  
Serological Examination ◽  
B Virus ◽  
The Right ◽  
Test Result

<p><em>Examination of the hepatitis B virus as a disease of hepatitis serologically has been carried out a lot, but this serological examination can experience problems if there is a low window period, so even though a negative test result is still possible in a patient's body infected with the hepatitis B virus, a fast and accurate examination is needed. PCR examination is an alternative solution to overcome this problem because the results are more accurate and valid because it can directly detect hepatitis B virus DNA as the cause of hepatitis B infection. This examination requires selecting the right primer so that accurate results are obtained. The aim of this study was to compare the use of HBS1 and HBS2 primers with P7 and P8 primers in the detection of Hepatitis B virus DNA in blood donors. The method used in this study is a laboratory experiment with Polymeration Chain Reaction (PCR) examination. The results showed that the HBS1 and HBS2 primers produced more positive HBV DNA by 76.92% compared to P7 and P8 primers, only 23.08%. Conclusion HBS1 and HBS2 primers can detect positive HBV DNA with more results.</em><em></em></p>

scholarly journals Hepatitis B virus DNA (HBV-DNA) in anti-HBc positive blood donors.

1992 ◽  
Vol 38 (3) ◽  
pp. 429-434
Author(s):  
Hiromi Ihara ◽  
Toshiaki Kato ◽  
Hisami Ikeda ◽  
Sadayoshi Sekiguchi
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Blood Donors ◽  
Hbv Dna ◽  
Hepatitis B Virus Dna ◽  
B Virus

Hepatitis B virus DNA in serum of ‘anti-HBc only’-positive healthy Lebanese blood donors: significance and possible implications

2007 ◽  
Vol 66 (3) ◽  
pp. 278-282 ◽  
Author(s):  
M. El-Zaatari ◽  
H. Kazma ◽  
M. Naboulsi-Majzoub ◽  
M. Haidar ◽  
F. Ramlawi ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Blood Donors ◽  
Hepatitis B Virus Dna ◽  
B Virus

The risk of hepatitis B virus infection by transfusion in Kumasi, Ghana

Blood ◽  
2003 ◽  
Vol 101 (6) ◽  
pp. 2419-2425 ◽  
Author(s):  
Jean-Pierre Allain ◽  
Daniel Candotti ◽  
Kate Soldan ◽  
Francis Sarkodie ◽  
Bruce Phelps ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Blood Donors ◽  
Hepatitis B Surface Antigen ◽  
Hbv Dna ◽  
Hbv Infection ◽  
Sub Saharan Africa ◽  
B Virus ◽  
Sub Saharan ◽  
Hbv Transmission

The risk of hepatitis B virus (HBV) transmission by transfusion in sub-Saharan Africa is considered to be relatively low, and testing of blood donors is often not done or is done relatively poorly. To re-examine this attitude, we identified HBV chronically infected blood donors from a major hospital in Ghana with a range of hepatitis B surface antigen (HBsAg) assays. Test efficacy was estimated using HBV DNA as a gold standard, and the risk of HBV infection in blood recipients was estimated for different testing strategies. Particle agglutination, dipstick, and enzyme immunoassay (EIA) HBsAg screening detected 54%, 71%, and 97% of HBV infectious donors, respectively. The risk of HBV transmission to recipients less than 10 years old ranged between 1:11 and 1:326 with blood unscreened and screened by EIA, respectively. For older recipients, the risk decreased a further 4-fold because of the high frequency of natural exposure to HBV. A total of 98% of HBsAg-confirmed positive samples contained HBV DNA. HBV DNA load was less than 1 × 104 IU/mL in 75% of HBsAg-reactive samples, most of them anti-HBe reactive. Approximately 0.5% of HBsAg-negative but anti-HBc-positive samples contained HBV DNA. The use of sensitive HBsAg tests is critical to prevent transfusion transmission of HBV infection to young children in a population with a 15% prevalence of chronic HBV infection in blood donors. However, this will not have much effect on the prevalence of this infection unless other strategies to protect children from infection are also advanced in parallel.

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