scholarly journals UJI BEBERAPA KONSENTRASI EKSTRAK TEPUNG DAUN BINTARO (Cerbera manghas L.) TERHADAP HAMA PENGGEREK TONGKOL JAGUNG MANIS (Helicoverpa armigera Hubner)

2020 ◽  
Vol 36 (1) ◽  
pp. 21-28
Author(s):  
Muslihat ◽  
Desita Salbiah
Keyword(s):  
Helicoverpa Armigera ◽  
Sweet Corn ◽  
Total Mortality ◽  
Effective Concentration ◽  
Initial Time ◽  
Botanical Insecticide ◽  
Helicoverpa Armígera ◽  
Lethal Time ◽  
Cerbera Manghas ◽  
Helicoverpa Armigera Hubner

ABSTRACT  The main pest that often attacks sweet corn crops is Helicoverpa armigera Hubner and causes damage. Pest control can be done using botanical insecticide bintaro plants (Cerbera manghas L.) The aim of this research was to get an effective concentration of bintaro leaf flour extract against of Helicoverpa armigera Hubner. The research was carried out experimentally at the Laboratory of Plant Pests, Agricultural Faculty, University of Riau, by using a completely randomized design (CRD) with four treatments and five replications. The treatments consisted of the several levels of concentration of bintaro leaf flour extract, 40 g.l-1 water, 60 g.l-1 water, 80 g.l-1 water and 100 g.l-1 water. The parameters observed were initial time of death (hours), lethal time 50 (hours), and total mortality (%). The results showed that bintaro leaf extract with a concentration of 80 g.l-1 water was an effective concentration to control H. armigera pests which could cause a total mortality of 82% with an initial death of 15,4 hours after application and lethal time 50 (LT50) 57,4 hours after application. Keywords: Helicoverpa armigera Hubner, Bintaro plants, Botanical insecticide

scholarly journals Comparative TMT Proteomic Analysis Unveils Unique Insights into Helicoverpa armigera (Hübner) Resistance in Cajanus scarabaeoides (L.) Thouars

2021 ◽  
Vol 22 (11) ◽  
pp. 5941
Author(s):  
Abigail Ngugi-Dawit ◽  
Isaac Njaci ◽  
Thomas J.V. Higgins ◽  
Brett Williams ◽  
Sita R. Ghimire ◽  
...  
Keyword(s):  
Host Plant ◽  
Helicoverpa Armigera ◽  
Plant Resistance ◽  
Host Plant Resistance ◽  
Proteomic Analysis ◽  
Artificial Diet ◽  
Growth Stages ◽  
Genetic Base ◽  
Helicoverpa Armígera ◽  
Helicoverpa Armigera Hubner

Pigeonpea [Cajanus cajan (L.) Millspaugh] is an economically important legume playing a crucial role in the semi-arid tropics. Pigeonpea is susceptible to Helicoverpa armigera (Hübner), which causes devastating yield losses. This pest is developing resistance to many commercially available insecticides. Therefore, crop wild relatives of pigeonpea, are being considered as potential sources of genes to expand the genetic base of cultivated pigeonpea to improve traits such as host plant resistance to pests and pathogens. Quantitative proteomic analysis was conducted using the tandem mass tag platform to identify differentially abundant proteins between IBS 3471 and ICPL 87 tolerant accession and susceptible variety to H. armigera, respectively. Leaf proteome were analysed at the vegetative and flowering/podding growth stages. H. armigera tolerance in IBS 3471 appeared to be related to enhanced defence responses, such as changes in secondary metabolite precursors, antioxidants, and the phenylpropanoid pathway. The development of larvae fed on an artificial diet with IBS 3471 lyophilised leaves showed similar inhibition with those fed on an artificial diet with quercetin concentrations with 32 mg/25 g of artificial diet. DAB staining (3,3’-diaminobenzidine) revealed a rapid accumulation of reactive oxygen species in IBS 3471. We conclude that IBS 3471 is an ideal candidate for improving the genetic base of cultivated pigeonpea, including traits for host plant resistance.

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