Sensitive and reliable detection of influenza A (H1N1), influenza A (H3N2) and influenza B by commercial real-time PCR assays: RIDA®GENE Flu and RIDA®GENE Flu LC2.0 real-time PCR

2016 ◽  
Author(s):  
Lena Kastl
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Influenza A ◽  
H1n1 Influenza ◽  
Influenza B ◽  
Influenza A H1n1 ◽  
Reliable Detection ◽  
Pcr Assays

scholarly journals Evaluation of four real-time PCR assays for detection of influenza A(H1N1)v viruses

2009 ◽  
Vol 14 (22) ◽  
Author(s):  
J Ellis ◽  
M Iturriza ◽  
R Allan ◽  
A Bermingham ◽  
K Brown ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Influenza A ◽  
Influenza Viruses ◽  
Influenza B ◽  
Influenza A Viruses ◽  
Concurrent Use ◽  
Influenza A H1n1 ◽  
Similar Range ◽  
Pcr Assays

The sensitivity and specificity of four real-time PCR assays (HPA A(H1)v, CDC A (H1)v, HPA A(N1)v and NVRL S-OIV assays) was evaluated for detection of influenza A(H1N1)v viruses. Nose and throat swab samples containing influenza A(H1N1)v viruses, seasonal influenza AH3N2, AH1N1, influenza B viruses, or negative for influenza viruses were tested by the four assays. Specificity was also analysed using influenza A viruses of different subtypes and non-related respiratory viruses. The sensitivities and specificities of the four assays were in a similar range and suitable for diagnostic use. The HPA (H1)v and the S-OIV assays were the most sensitive assays for use as a first line test, but the S-OIV assay was less specific, detecting all avian subtypes of influenza A viruses tested. The results of this study demonstrate that the concurrent use of primary diagnostic and confirmatory assays provides rapid and accurate assessment of confirmed cases, and allows appropriate management of patients.

scholarly journals Clinical Investigations. Comparison of Directigen Flu A+B with Real Time PCR in the Diagnosis of Influenza / Сравнение Иммунохроматографического Метода (Directigen Flu A+B) И Теста RT-PCR При Инфекциях Гриппа

Folia Medica ◽  
2015 ◽  
Vol 57 (2) ◽  
pp. 104-110 ◽  
Author(s):  
Golubinka Bosevska ◽  
Nikola Panovski ◽  
Elizabeta Janceska ◽  
Vladimir Mikik ◽  
Irena Kondova Topuzovska ◽  
...  
Keyword(s):  
Real Time ◽  
Influenza A Virus ◽  
Real Time Pcr ◽  
Influenza A ◽  
Age Groups ◽  
Influenza Viruses ◽  
Influenza B Virus ◽  
Influenza B ◽  
B Virus ◽  
Nose And Throat

AbstractEarly diagnosis and treatment of patients with influenza is the reason why physicians need rapid high-sensitivity influenza diagnostic tests that require no complex lab equipment and can be performed and interpreted within 15 min. The Aim of this study was to compare the rapid Directigen Flu A+B test with real time PCR for detection of influenza viruses in the Republic of Macedonia. MATERIALS AND METHODS: One-hundred-eight respiratory samples (combined nose and throat swabs) were routinely collected for detection of influenza virus during influenza seasons. Forty-one patients were pediatric cases and 59 were adult. Their mean age was 23 years. The patients were allocated into 6 age groups: 0 - 4 yrs, 5 - 9 yrs, 10 - 14 yrs, 15 - 19 yrs, 20-64 yrs and > 65 yrs. Each sample was tested with Directigen Flu A+B and CDC real time PCR kit for detection and typisation/subtypisation of influenza according to the lab diagnostic protocol. RESULTS: Directigen Flu A+B identified influenza A virus in 20 (18.5%) samples and influenza B virus in two 2 (1.9%) samples. The high specificity (100%) and PPV of Directigen Flu A+B we found in our study shows that the positive results do not need to be confirmed. The overall sensitivity of Directigen Flu A+B is 35.1% for influenza A virus and 33.0% for influenza B virus. The sensitivity for influenza A is higher among children hospitalized (45.0%) and outpatients (40.0%) versus adults. CONCLUSION: Directigen Flu A+B has relatively low sensitivity for detection of influenza viruses in combined nose and throat swabs. Negative results must be confirmed.

scholarly journals Comparison of Becton Dickinson Directigen EZ Flu A+B Test against the CDC Real-Time PCR Assay for Detection of 2009 Pandemic Influenza A/H1N1 Virus: TABLE 1.

2009 ◽  
Vol 48 (1) ◽  
pp. 343-344 ◽  
Author(s):  
Tess Karre ◽  
Hugh F. Maguire ◽  
David Butcher ◽  
Amy Graepler ◽  
Diane Weed ◽  
...  
Keyword(s):  
Real Time ◽  
Pandemic Influenza ◽  
Real Time Pcr ◽  
Influenza A ◽  
H1n1 Virus ◽  
Pcr Assay ◽  
Becton Dickinson ◽  
Influenza A H1n1 ◽  
B Test

scholarly journals Library of Prefabricated Locked Nucleic Acid Hydrolysis Probes Facilitates Rapid Development of Reverse-Transcription Quantitative Real-Time PCR Assays for Detection of Novel Influenza A/H1N1/09 Virus

2009 ◽  
Vol 55 (12) ◽  
pp. 2218-2222 ◽  
Author(s):  
Jürgen J Wenzel ◽  
Heiko Walch ◽  
Markus Bollwein ◽  
Hans Helmut Niller ◽  
Waltraud Ankenbauer ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Reverse Transcription ◽  
Real Time Pcr ◽  
Influenza A ◽  
Rapid Development ◽  
Locked Nucleic Acid ◽  
Quantitative Real Time Pcr ◽  
Influenza A H1n1 ◽  
Novel Influenza A

Abstract Background: The emergence of a novel pandemic human strain of influenza A (H1N1/09) has clearly demonstrated the need for flexible tools enabling the rapid development of new diagnostic methods. Methods: We designed a set of reverse-transcription quantitative real-time PCR (RT-qPCR) assays based on the Universal ProbeLibrary (UPL)—a collection of 165 presynthesized, fluorescence-labeled locked nucleic acid (LNA) hydrolysis probes—specifically to detect the novel influenza A virus. We evaluated candidate primer/UPL-probe pairs with 28 novel influenza A/H1N1/09 patient samples of European and Mexican origin. Results: Of 14 assays in the hemagglutinin (HA) and neuraminidase (NA) genes, 12 detected viral nucleic acids from diluted patient samples without need for further optimization. We characterized the diagnostic specificity of the 2 best-performing assays with a set of samples comprising various influenza virus strains of human and animal origin that showed no cross-reactivity. The diagnostic sensitivity of these 2 primer/probe combinations was in the range of 100–1000 genomic copies/mL. In comparison to a reference assay recommended by the German health authorities, the analytical sensitivities and specificities of the assays were equivalent. Conclusions: Facing the emergence of novel influenza A/H1N1/09, we were able to develop, within 2 days, a set of sensitive and specific RT-qPCR assays for the laboratory diagnosis of suspected cases. H1N1/09 served as a model to show the feasibility of the UPL approach for the expedited development of new diagnostic assays to detect emerging pathogens.

scholarly journals Evaluation of Real-Time Reverse Transcriptase PCR Assays for Detection of Pandemic Influenza A/H1N1 2009 Virus

2011 ◽  
Vol 49 (9) ◽  
pp. 3444-3445 ◽  
Author(s):  
G. R. Chidlow ◽  
G. B. Harnett ◽  
D. J. Speers ◽  
D. W. Smith
Keyword(s):  
Reverse Transcriptase ◽  
Real Time ◽  
Pandemic Influenza ◽  
Influenza A ◽  
Reverse Transcriptase Pcr ◽  
Influenza A H1n1 ◽  
Pcr Assays
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