Anchored multiplex PCR enables sensitive NGS-based mutation detection in the context of large primer panels

2018 ◽  
Author(s):  
Josh Haimes
Keyword(s):  
Multiplex Pcr ◽  
Mutation Detection

Fast and Efficient Mutation Detection Method Using Multiplex PCR and Cycle Sequencing

2000 ◽  
Vol 83 (02) ◽  
pp. 244-247 ◽  
Author(s):  
J. M. Costa ◽  
P. Ernault ◽  
D. Vidaud ◽  
M. Vidaud ◽  
D. Meyer ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Mutation Detection ◽  
Detection Method ◽  
Deleterious Mutation ◽  
Low Cost ◽  
Detection Methods ◽  
Cycle Sequencing ◽  
Haemophilia B ◽  
Mild Haemophilia

SummaryA method using multiplex PCR followed by cycle-sequencing has been developed to detect mutations in the FIX gene. The procedure was evaluated in 45 severe or mild haemophilia B patients from 45 unrelated families. At least one deleterious mutation was identified in every haemophiliac demonstrating the efficiency of the method. Furthermore the described procedure offers many advantages compared to other screening detection methods: it is fast (less than 48 h), simple (partly automated) and of relatively low cost (it requires only one PCR).

scholarly journals IDH Mutation Detection in Formalin-Fixed Paraffin-Embedded Gliomas Using Multiplex PCR and Single-Base Extension

2012 ◽  
Vol 22 (5) ◽  
pp. 619-624 ◽  
Author(s):  
Marco Perizzolo ◽  
Bob Winkfein ◽  
Susan Hui ◽  
Wally Krulicki ◽  
Jennifer A. Chan ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Mutation Detection ◽  
Idh Mutation ◽  
Single Base Extension ◽  
Single Base ◽  
Base Extension ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

scholarly journals Multiplex PCR-SSCP: A highly effective and efficient method of mutation detection and analysis

1998 ◽  
Vol 4 (2) ◽  
pp. 135 ◽  
Author(s):  
Xin Zhao ◽  
Yu-Yang Cai ◽  
Da-Wen Xie ◽  
Li-Dong Wang ◽  
Chung S Yang
Keyword(s):  
Multiplex Pcr ◽  
Efficient Method ◽  
Mutation Detection ◽  
Highly Effective

A multiplex PCR-SSCP test to genotype bovine β-casein alleles A1 , A2 , A3 , B, and C

1999 ◽  
Vol 30 (4) ◽  
pp. 322-323 ◽  
Author(s):  
A. Barroso ◽  
S. Dunner ◽  
J. Ca·ón
Keyword(s):  
Multiplex Pcr

Multiplex PCR zum Nachweis disseminierter Tumorzellen (DTC) im Blut von Patientinnen mit Mammakarzinom

2005 ◽  
Vol 127 (03) ◽  
Author(s):  
J Rom ◽  
A Schneeeweiss ◽  
V Zieglschmid ◽  
C Hollmann ◽  
O Böcher ◽  
...  
Keyword(s):  
Multiplex Pcr
Sign in / Sign up

Export Citation Format

Share Document