Automated transcription profiling to monitor malaria parasite sexual differentiation

2021 ◽  
Author(s):  
Abel Cruz Camacho ◽  
Edo Kiper
Keyword(s):  
Sexual Differentiation ◽  
Malaria Parasite ◽  
Transcription Profiling

Commitment to sexual differentiation in the human malaria parasite, Plasmodium falciparum

Parasitology ◽  
2000 ◽  
Vol 121 (2) ◽  
pp. 127-133 ◽  
Author(s):  
T. G. SMITH ◽  
P. LOURENÇO ◽  
R. CARTER ◽  
D. WALLIKER ◽  
L. C. RANFORD-CARTWRIGHT
Keyword(s):  
Plasmodium Falciparum ◽  
Sexual Differentiation ◽  
Malaria Parasite ◽  
Human Malaria Parasite ◽  
Human Malaria ◽  
Parasite Plasmodium ◽  
Parasite Plasmodium Falciparum

scholarly journals Multistage activity within a diverse set of epi-drugs against Plasmodium falciparum parasites

2019 ◽  
Author(s):  
Nanika Coetzee ◽  
Hilde von Grüning ◽  
Mariette van der Watt ◽  
Janette Reader ◽  
Lyn-Marié Birkholtz
Keyword(s):  
Plasmodium Falciparum ◽  
Sexual Development ◽  
Sexual Differentiation ◽  
Late Stage ◽  
Malaria Parasite ◽  
Early Stage ◽  
Asexual Development ◽  
Epigenetic Modifiers ◽  
Cancerous Cells ◽  
Multiple Stages

AbstractThe epigenome of the malaria parasite, Plasmodium falciparum, is associated with control of various essential processes in the parasite including control of proliferation of asexual development as well as sexual differentiation. The unusual nature of the epigenome has prompted investigations of the potential to target epigenetic modulators with novel chemotypes. Here, we explored the diversity associated with a library of 95 compounds, active against various epigenetic modifiers within cancerous cells, for activity against multiple stages of P. falciparum development. We show that P. falciparum is differentially susceptible to epigenetic perturbation during asexual and sexual development, with early stage gametocytes particularly sensitive to epi-drugs targeting both histone and non-histone epigenetic modifiers. Moreover, 4 compounds targeting histone acetylation and methylation, show potent multistage activity against asexual parasites, early and late stage gametocytes, with transmission-blocking potential. Overall, these results warrant further examination of the potential antimalarial properties of these hit compounds.

scholarly journals Hierarchical transcriptional control regulatesPlasmodium falciparumsexual differentiation

2019 ◽  
Author(s):  
Riëtte van Biljon ◽  
Roelof van Wyk ◽  
Heather J. Painter ◽  
Lindsey Orchard ◽  
Janette Reader ◽  
...  
Keyword(s):  
Sexual Differentiation ◽  
Malaria Parasite ◽  
Transcriptional Control ◽  
Molecular Mechanisms ◽  
Epigenetic Mechanisms ◽  
Critical Stage ◽  
Functional Studies ◽  
Transcriptional Dynamics ◽  
Differential Timing ◽  
Asexual Cycle

AbstractMalaria pathogenesis relies on sexual gametocyte forms of the malaria parasite to be transmitted between the infected human and the mosquito host but the molecular mechanisms controlling gametocytogenesis remains poorly understood. Here we provide a high-resolution transcriptome ofPlasmodium falciparumas it commits to and develops through gametocytogenesis. The gametocyte-associated transcriptome is significantly different from that of the asexual parasites, with dynamic gene expression shifts characterizing early, intermediate and late-stage gametocyte development and results in differential timing for sex-specific transcripts. The striking transcriptional dynamics suggest strict transcriptional control during gametocytogenesis inP. falciparum, which we propose is mediated by putative regulators including epigenetic mechanisms (driving active repression of proliferation-associated processes) and a cascade-like expression of ApiAP2 transcription factors. The gametocyte transcriptome serves as the blueprint for sexual differentiation and will be a rich resource for future functional studies on this critical stage ofPlasmodiumdevelopment, as the intraerythrocytic transcriptome has been for our understanding of the asexual cycle.

scholarly journals Hierarchical transcriptional control regulates Plasmodium falciparum sexual differentiation

2019 ◽  
Author(s):  
Riëtte van Biljon ◽  
Roelof van Wyk ◽  
Heather J. Painter ◽  
Lindsey Orchard ◽  
Janette Reader ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Sexual Differentiation ◽  
Malaria Parasite ◽  
Transcriptional Control ◽  
Molecular Mechanisms ◽  
Critical Stage ◽  
Functional Studies ◽  
Transcriptional Dynamics ◽  
Differential Timing ◽  
Asexual Cycle

Abstract Background Malaria pathogenesis relies on sexual gametocyte forms of the malaria parasite to be transmitted between the infected human and the mosquito host but the molecular mechanisms controlling gametocytogenesis remains poorly understood. Here we provide a high-resolution transcriptome of Plasmodium falciparum as it commits to and develops through gametocytogenesis. Results The gametocyte-associated transcriptome is significantly different from that of the asexual parasites, with dynamic gene expression shifts characterizing early, intermediate and late-stage gametocyte development and results in differential timing for sex-specific transcripts. The transcriptional dynamics suggest strict transcriptional control during gametocytogenesis in P. falciparum, which we propose is mediated by putative regulators including epigenetic mechanisms (driving active repression of proliferation-associated processes) and a cascade-like expression of ApiAP2 transcription factors. Conclusions The gametocyte transcriptome serves as the blueprint for sexual differentiation and will be a rich resource for future functional studies on this critical stage of Plasmodium development, as the intraerythrocytic transcriptome has been for our understanding of the asexual cycle.

scholarly journals Hierarchical transcriptional control regulates Plasmodium falciparum sexual differentiation

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Riëtte van Biljon ◽  
Roelof van Wyk ◽  
Heather J. Painter ◽  
Lindsey Orchard ◽  
Janette Reader ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Sexual Differentiation ◽  
Malaria Parasite ◽  
Transcriptional Control ◽  
Molecular Mechanisms ◽  
Critical Stage ◽  
Functional Studies ◽  
Transcriptional Dynamics ◽  
Differential Timing ◽  
Asexual Cycle

Abstract Background Malaria pathogenesis relies on sexual gametocyte forms of the malaria parasite to be transmitted between the infected human and the mosquito host but the molecular mechanisms controlling gametocytogenesis remains poorly understood. Here we provide a high-resolution transcriptome of Plasmodium falciparum as it commits to and develops through gametocytogenesis. Results The gametocyte-associated transcriptome is significantly different from that of the asexual parasites, with dynamic gene expression shifts characterizing early, intermediate and late-stage gametocyte development and results in differential timing for sex-specific transcripts. The transcriptional dynamics suggest strict transcriptional control during gametocytogenesis in P. falciparum, which we propose is mediated by putative regulators including epigenetic mechanisms (driving active repression of proliferation-associated processes) and a cascade-like expression of ApiAP2 transcription factors. Conclusions The gametocyte transcriptome serves as the blueprint for sexual differentiation and will be a rich resource for future functional studies on this critical stage of Plasmodium development, as the intraerythrocytic transcriptome has been for our understanding of the asexual cycle.

scholarly journals Hierarchical transcriptional control regulates Plasmodium falciparum sexual differentiation

2019 ◽  
Author(s):  
Riëtte van Biljon ◽  
Roelof van Wyk ◽  
Heather J. Painter ◽  
Lindsey Orchard ◽  
Janette Reader ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Sexual Differentiation ◽  
Malaria Parasite ◽  
Transcriptional Control ◽  
Molecular Mechanisms ◽  
Critical Stage ◽  
Functional Studies ◽  
Transcriptional Dynamics ◽  
Differential Timing ◽  
Asexual Cycle

Abstract Background: Malaria pathogenesis relies on sexual gametocyte forms of the malaria parasite to be transmitted between the infected human and the mosquito host but the molecular mechanisms controlling gametocytogenesis remains poorly understood. Here we provide a high-resolution transcriptome of Plasmodium falciparum as it commits to and develops through gametocytogenesis. Results : The gametocyte-associated transcriptome is significantly different from that of the asexual parasites, with dynamic gene expression shifts characterizing early, intermediate and late-stage gametocyte development and results in differential timing for sex-specific transcripts. The transcriptional dynamics suggest strict transcriptional control during gametocytogenesis in P. falciparum, which we propose is mediated by putative regulators including epigenetic mechanisms (driving active repression of proliferation-associated processes) and a cascade-like expression of ApiAP2 transcription factors. Conclusions : The gametocyte transcriptome serves as the blueprint for sexual differentiation and will be a rich resource for future functional studies on this critical stage of Plasmodium development, as the intraerythrocytic transcriptome has been for our understanding of the asexual cycle.

scholarly journals Hierarchical transcriptional control regulates Plasmodium falciparum sexual differentiation

2019 ◽  
Author(s):  
Riëtte van Biljon ◽  
Roelof van Wyk ◽  
Heather J. Painter ◽  
Lindsey Orchard ◽  
Janette Reader ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Sexual Differentiation ◽  
Malaria Parasite ◽  
Transcriptional Control ◽  
Molecular Mechanisms ◽  
Critical Stage ◽  
Functional Studies ◽  
Transcriptional Dynamics ◽  
Differential Timing ◽  
Asexual Cycle

Abstract Background: Malaria pathogenesis relies on sexual gametocyte forms of the malaria parasite to be transmitted between the infected human and the mosquito host but the molecular mechanisms controlling gametocytogenesis remains poorly understood. Here we provide a high-resolution transcriptome of Plasmodium falciparum as it commits to and develops through gametocytogenesis. Results : The gametocyte-associated transcriptome is significantly different from that of the asexual parasites, with dynamic gene expression shifts characterizing early, intermediate and late-stage gametocyte development and results in differential timing for sex-specific transcripts. The transcriptional dynamics suggest strict transcriptional control during gametocytogenesis in P. falciparum, which we propose is mediated by putative regulators including epigenetic mechanisms (driving active repression of proliferation-associated processes) and a cascade-like expression of ApiAP2 transcription factors. Conclusions : The gametocyte transcriptome serves as the blueprint for sexual differentiation and will be a rich resource for future functional studies on this critical stage of Plasmodium development, as the intraerythrocytic transcriptome has been for our understanding of the asexual cycle.

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