scholarly journals Liquid-Phase Peak Force Infrared Microscopy for Chemical Nano-imaging and Spectroscopy of Soft Matters

2020 ◽  
Author(s):  
Haomin Wang ◽  
Joseph M. González-Fialkowski ◽  
Wenqian Li ◽  
Qing Xie ◽  
Yan Yu ◽  
...  
Keyword(s):  
Liquid Phase ◽  
Infrared Imaging ◽  
Polymer Surface ◽  
Fluid Phase ◽  
Peak Force ◽  
Label Free ◽  
Imaging And Spectroscopy ◽  
Nano Imaging ◽  
Solid Liquid Interface ◽  
Soft Matters

<a>Peak force infrared (PFIR) microscopy is an emerging atomic force microscopy that bypasses Abbe’s diffraction limit in achieving chemical nano-imaging and spectroscopy. The PFIR microscopy mechanically detects the infrared photothermal responses in the dynamic tip-sample contact of peak force tapping mode, and has been applied for a variety of samples, ranging from soft matters, photovoltaics heterojunctions, to polaritonic materials under the air conditions. In this article, we develop and demonstrate the PFIR microscopy in the liquid phase for soft matters and biological samples. With the capability of controlling fluid compositions on demand, the liquid-phase peak force infrared (LiPFIR) microscopy enables <i>in situ </i>tracking the polymer surface reorganization in fluids and detecting the product of click chemical reaction in the aqueous phase. Both broadband spectroscopy and infrared imaging with ~ 10 nm spatial resolution are benchmarked in the fluid phase, together with complementary mechanical information. We also demonstrate the LiPFIR microscopy on revealing the chemical composition of a budding site of yeast cell wall particles in water as an application on biological structures. The label-free, non-destructive chemical nano-imaging and spectroscopic capabilities of the LiPFIR microscopy will facilitate the investigations of soft matters and their transformations at the solid/liquid interface.</a>

scholarly journals Liquid-Phase Peak Force Infrared Microscopy for Chemical Nano-imaging and Spectroscopy of Soft Matters

2020 ◽  
Author(s):  
Haomin Wang ◽  
Joseph M. González-Fialkowski ◽  
Wenqian Li ◽  
Qing Xie ◽  
Yan Yu ◽  
...  
Keyword(s):  
Liquid Phase ◽  
Infrared Imaging ◽  
Polymer Surface ◽  
Fluid Phase ◽  
Peak Force ◽  
Label Free ◽  
Imaging And Spectroscopy ◽  
Nano Imaging ◽  
Solid Liquid Interface ◽  
Soft Matters

<a>Peak force infrared (PFIR) microscopy is an emerging atomic force microscopy that bypasses Abbe’s diffraction limit in achieving chemical nano-imaging and spectroscopy. The PFIR microscopy mechanically detects the infrared photothermal responses in the dynamic tip-sample contact of peak force tapping mode, and has been applied for a variety of samples, ranging from soft matters, photovoltaics heterojunctions, to polaritonic materials under the air conditions. In this article, we develop and demonstrate the PFIR microscopy in the liquid phase for soft matters and biological samples. With the capability of controlling fluid compositions on demand, the liquid-phase peak force infrared (LiPFIR) microscopy enables <i>in situ </i>tracking the polymer surface reorganization in fluids and detecting the product of click chemical reaction in the aqueous phase. Both broadband spectroscopy and infrared imaging with ~ 10 nm spatial resolution are benchmarked in the fluid phase, together with complementary mechanical information. We also demonstrate the LiPFIR microscopy on revealing the chemical composition of a budding site of yeast cell wall particles in water as an application on biological structures. The label-free, non-destructive chemical nano-imaging and spectroscopic capabilities of the LiPFIR microscopy will facilitate the investigations of soft matters and their transformations at the solid/liquid interface.</a>

Liquid-Phase Peak Force Infrared Microscopy

2020 ◽  
Author(s):  
Haomin Wang ◽  
Joseph M. González-Fialkowski ◽  
Wenqian Li ◽  
Yan Yu ◽  
Xiaoji Xu
Keyword(s):  
Liquid Phase ◽  
Spatial Resolution ◽  
Shear Force ◽  
Polymer Surface ◽  
Surface Damage ◽  
Peak Force ◽  
Infrared Microscopy ◽  
Contact Mode ◽  
Force Microscopy ◽  
Hydrogen Deuterium

Atomic force microscopy-infrared microscopy (AFM-IR) provides a route to bypass Abbe’s diffraction limit through photothermal detections of infrared absorption. With the combination of total internal reflection, AFM-IR can operate in the aqueous phase. However, AFM-IR in contact mode suffers from surface damage from the lateral shear force between the tip and sample, and can only achieve 20~25-nm spatial resolution. Here, we develop the liquid-phase peak force infrared (LiPFIR) microscopy that avoids the detrimental shear force and delivers an 8-nm spatial resolution. The non-destructiveness of the LiPFIR microscopy enables <i>in situ</i> chemical measurement of heterogeneous materials and investigations on a range of chemical and physical transformations, including polymer surface reorganization, hydrogen-deuterium isotope exchange, and ethanol-induced denaturation of proteins. We also perform LiPFIR imaging of the budding site of yeast cell wall in the fluid as a demonstration of biological applications. LiPFIR unleashes the potential of in liquid AFM-IR for chemical nanoscopy.

scholarly journals Liquid-Phase Peak Force Infrared Microscopy

2020 ◽  
Author(s):  
Haomin Wang ◽  
Joseph M. González-Fialkowski ◽  
Wenqian Li ◽  
Qing Xie ◽  
Yan Yu ◽  
...  
Keyword(s):  
Liquid Phase ◽  
Spatial Resolution ◽  
Shear Force ◽  
Polymer Surface ◽  
Surface Damage ◽  
Peak Force ◽  
Infrared Microscopy ◽  
Contact Mode ◽  
Force Microscopy ◽  
Hydrogen Deuterium

Atomic force microscopy-infrared microscopy (AFM-IR) provides a route to bypass Abbe’s diffraction limit through photothermal detections of infrared absorption. With the combination of total internal reflection, AFM-IR can operate in the aqueous phase. However, AFM-IR in contact mode suffers from surface damage from the lateral shear force between the tip and sample, and can only achieve 20~25-nm spatial resolution. Here, we develop the liquid-phase peak force infrared (LiPFIR) microscopy that avoids the detrimental shear force and delivers an 8-nm spatial resolution. The non-destructiveness of the LiPFIR microscopy enables <i>in situ</i> chemical measurement of heterogeneous materials and investigations on a range of chemical and physical transformations, including polymer surface reorganization, hydrogen-deuterium isotope exchange, and ethanol-induced denaturation of proteins. We also perform LiPFIR imaging of the budding site of yeast cell wall in the fluid as a demonstration of biological applications. LiPFIR unleashes the potential of in liquid AFM-IR for chemical nanoscopy.

Capturing Protein Droplets: Label-free Visualization and Detection of Protein Liquid-Liquid Phase Separation with an Aggregation-Induced Emission Fluorogen

2021 ◽  
Author(s):  
Yan-Mei Li ◽  
Chu-Qiao Liang ◽  
Lin Wang ◽  
Yun-Yi Luo ◽  
Qian-Qian Li
Keyword(s):  
Phase Separation ◽  
Liquid Phase ◽  
Liquid Phase Separation ◽  
New Method ◽  
Label Free ◽  
Simple Method ◽  
Aggregation Induced Emission ◽  
Liquid Liquid Phase Separation

We developed a new method for protein droplet visualization by means of a droplet probe (DroProbe) based on an aggregation-induced emission (AIE) fluorogen. A simple method for viscosity comparison of...

Role of the solid/liquid interface faceting in rapid penetration of a liquid phase along grain boundaries

2001 ◽  
Vol 49 (7) ◽  
pp. 1123-1128 ◽  
Author(s):  
D. Chatain ◽  
E. Rabkin ◽  
J. Derenne ◽  
J. Bernardini
Keyword(s):  
Liquid Phase ◽  
Grain Boundaries ◽  
Liquid Interface ◽  
Solid Liquid Interface ◽  
Solid Liquid

scholarly journals Chalcogenide Microstructured Optical Fibers for Mid-Infrared Supercontinuum Generation: Interest, Fabrication, and Applications

2018 ◽  
Vol 8 (9) ◽  
pp. 1637 ◽  
Author(s):  
Yiming Wu ◽  
Marcello Meneghetti ◽  
Johann Troles ◽  
Jean-Luc Adam
Keyword(s):  
Optical Fibers ◽  
Infrared Imaging ◽  
Supercontinuum Generation ◽  
Mid Infrared ◽  
Microstructured Fibers ◽  
Research Fields ◽  
Infrared Spectral ◽  
Purification Methods ◽  
Imaging And Spectroscopy ◽  
High Coherence

The mid-infrared spectral region is of great technical and scientific importance in a variety of research fields and applications. Among these studies, mid-infrared supercontinuum generation has attracted strong interest in the last decade, because of unique properties such as broad wavelength coverage and high coherence, among others. In this paper, the intrinsic optical properties of different types of glasses and fibers are presented. It turns out that microstructured chalcogenide fibers are ideal choices for the generation of mid-infrared supercontinua. The fabrication procedures of chalcogenide microstructured fibers are introduced, including purification methods of the glass, rod synthesis processes, and preform realization techniques. In addition, supercontinua generated in chalcogenide microstructured fibers employing diverse pump sources and configurations are enumerated. Finally, the potential of supercontinua for applications in mid-infrared imaging and spectroscopy is shown.

scholarly journals Label-free digital pathology by infrared imaging

2020 ◽  
Vol 9 (1-2) ◽  
pp. 5-12 ◽  
Author(s):  
Frederik Großerueschkamp ◽  
Klaus Gerwert
Keyword(s):  
Infrared Imaging ◽  
Digital Pathology ◽  
Label Free
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