scholarly journals Manufacturing of Liposomes: A Direct Comparison of Extrusion and Microfluidics Protocols

2021 ◽  
Author(s):  
Mahmoud Tarek Sanad ◽  
Claudio Alter ◽  
Pascal Detampel ◽  
Tomaz Einfalt ◽  
Jörg Huwyler
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Scale Up ◽  
Preparation Methods ◽  
Manufacturing Method ◽  
Liposomal Formulations ◽  
Liposome Preparation ◽  
Identical Composition ◽  
Manufacturing Methods ◽  
Parenteral Drug

Liposomal formulations are frequently used for oral, topical, or parenteral drug administration. However, liposome manufacturing and industrial scale-up remains a challenge, in particular if it comes to the preparation of liposome populations with a homogenous size distribution. Therefore, extrusion through filter membranes with defined pore size is traditionally used during the preparation of small unilamellar liposomes. Microfluidics is considered to be an alternative manufacturing method. Lipids, solvents and excipients are thereby passively mixed using a microfluidics device. While the microfluidic approach is highly scalable, most of the traditional liposome preparation protocols rely on extrusion. It was therefore the aim of the present study to compare liposomal formulations with identical composition, which were prepared using either extrusion or microfluidics protocols. Liposomal formulations produced by both methods were analyzed using dynamic light scattering (DLS) to compare size, polydispersity, and ζ-potential. Our results indicate significant differences between liposomal preparations obtained using the two manufacturing methods. We conclude that the two preparation methods should not be used interchangeably.<br>

scholarly journals Manufacturing of Liposomes: A Direct Comparison of Extrusion and Microfluidics Protocols

2021 ◽  
Author(s):  
Mahmoud Tarek Sanad ◽  
Claudio Alter ◽  
Pascal Detampel ◽  
Tomaz Einfalt ◽  
Jörg Huwyler
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Scale Up ◽  
Preparation Methods ◽  
Manufacturing Method ◽  
Liposomal Formulations ◽  
Liposome Preparation ◽  
Identical Composition ◽  
Manufacturing Methods ◽  
Parenteral Drug

Liposomal formulations are frequently used for oral, topical, or parenteral drug administration. However, liposome manufacturing and industrial scale-up remains a challenge, in particular if it comes to the preparation of liposome populations with a homogenous size distribution. Therefore, extrusion through filter membranes with defined pore size is traditionally used during the preparation of small unilamellar liposomes. Microfluidics is considered to be an alternative manufacturing method. Lipids, solvents and excipients are thereby passively mixed using a microfluidics device. While the microfluidic approach is highly scalable, most of the traditional liposome preparation protocols rely on extrusion. It was therefore the aim of the present study to compare liposomal formulations with identical composition, which were prepared using either extrusion or microfluidics protocols. Liposomal formulations produced by both methods were analyzed using dynamic light scattering (DLS) to compare size, polydispersity, and ζ-potential. Our results indicate significant differences between liposomal preparations obtained using the two manufacturing methods. We conclude that the two preparation methods should not be used interchangeably.<br>

scholarly journals Preparation and Characterization of Two Different Liposomal Formulations with Bioactive Natural Extract for Multiple Applications

Processes ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 432
Author(s):  
Florina Miere (Groza) ◽  
Simona Ioana Vicas ◽  
Adrian Vasile Timar ◽  
Mariana Ganea ◽  
Mihaela Zdrinca ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Encapsulation Efficiency ◽  
The Body ◽  
Total Phenols ◽  
Stellaria Media ◽  
Lipid Film ◽  
Natural Extract ◽  
Liposomal Formulations

Liposomes continue to attract great interest due to their increased bioavailability in the body and because the substances encapsulated are protected while maintaining their effectiveness. The aim of this study is to obtain “giant” liposomes by lipid film hydration using a preparation formula with two different phospholipids, phosphatidylcholine (PC) and phosphatidylserine (PS). Firstly, the macro- and microscopic characterization, total phenols content and antioxidant capacity of the plant Stellaria media (L.) Vill. were assessed. Then, Stellaria media (L.) Vill. extract was encapsulated in both formulations (PCE and PSE) and the liposomes were characterized according to their morphology, size distribution and Zeta potential using optical microscopy and dynamic light scattering. The encapsulation efficiency (EE%) was determined using the Folin–Ciocalteu method and the values of both formulations were compared. PC and PCE liposomes with a diameter between 712 and 1000 nm and PS and PSE liposomes with a diameter between 58 and 1000 nm were obtained. The values EE% of Stellaria media (L.) Vill. extract for PCE and PSE were 92.09% and 84.25%, respectively.

Dynamic light scattering from gels in a poor solvent

1978 ◽  
Vol 39 (9) ◽  
pp. 955-960 ◽  
Author(s):  
E. Geissler ◽  
A.M. Hecht
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Poor Solvent

DYNAMIC LIGHT SCATTERING IN LC-440 NEMATIC LIQUID CRYSTAL

2014 ◽  
Vol 73 (11) ◽  
pp. 977-983
Author(s):  
A. P. Fedoryako ◽  
A. I. Kocherzhin ◽  
M. P. Kukhtin ◽  
E. I. Chernyakov
Keyword(s):  
Liquid Crystal ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Nematic Liquid Crystal

Synthesis of Calcium Carbonate Micro Particles using Emulsion Membrane Process Applied for Drug Release Studies

2017 ◽  
Vol 68 (10) ◽  
pp. 2320-2324
Author(s):  
Mariana Mateescu ◽  
Sanda Maria Doncea ◽  
Irina Chican ◽  
Cristina Lavinia Nistor ◽  
Ioneta Codrina Bujanca
Keyword(s):  
Light Scattering ◽  
Calcium Carbonate ◽  
Dynamic Light Scattering ◽  
Liquid Membranes ◽  
Membrane Process ◽  
Emulsion Method ◽  
Micro Particles ◽  
Release Studies ◽  
Emulsion Liquid Membranes ◽  
Span 80

The aim of this work is the synthesis of calcium carbonate (CaCO3) nano and microparticles and their application as biomaterials (vehicles) for the sustained release of doxycycline. CaCO3 micro particles were synthesized by water-in oil (W/O) emulsion method using emulsion liquid membranes with bis (2-ethylhexyl) phosphate (D2EHPA) as carrier, Span 80 as surfactant, and toluene and kerosene as organic solvents. The aqueous phases contained 1 M CaCl2, and 1 M Na2CO3, respectively. The Dynamic Light Scattering (DLS) data showed CaCO3 particles with sizes ranging from around 100 nm to 3500 nm. The CaCO3 particles with the average diameters around 600 nm attained an adsorbtion of doxycycline of maximum 97.9%, and a slow and steady release with a cumulative value of approximative 50% after ten days.

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