Fatty Acid Fueled Transmembrane Chloride Transport

Fatty Acid-Fuelled Transmembrane Chloride Transport

10.26434/chemrxiv.6823352.v1 ◽

2018 ◽

Author(s):

Philip Gale ◽

Ethan N.W.Howe

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Lipid Bilayer ◽

Chloride Transport ◽

Bilayer Membrane ◽

Lipid Bilayer Membrane ◽

Ph Gradient ◽

Pumping System ◽

Membrane Addition

We report an example of the use of fatty acids to drive chloride transport by creating a pH gradient across a vesicular lipid bilayer membrane. Addition of an unselective squaramide-based chloride transporter (which transports both H+and Cl-) facilitates the transport of HCl from the vesicle (driven by the pH gradient) so creating a chloride gradient. Addition of further aliquots of fatty acid ‘fuel’ can initiate further transport of chloride out of the vesicle by re-establishing the pH gradient. This is an example of a prototypical chloride pumping system.

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Fatty Acid Fueled Transmembrane Chloride Transport

10.26434/chemrxiv.6823352.v2 ◽

2019 ◽

Author(s):

Ethan N.W. Howe ◽

Philip Gale

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Lipid Bilayer ◽

Chloride Transport ◽

Bilayer Membrane ◽

Lipid Bilayer Membrane ◽

Ph Gradient ◽

Pumping System ◽

Membrane Addition

We report an example of the use of fatty acids to drive chloride transport by creating a pH gradient across a vesicular lipid bilayer membrane. Addition of an unselective squaramide-based chloride transporter (which transports both H+and Cl-) facilitates the transport of HCl from the vesicle (driven by the pH gradient) so creating a chloride gradient. Addition of further aliquots of fatty acid ‘fuel’ can initiate further transport of chloride out of the vesicle by re-establishing the pH gradient. This is an example of a prototypical chloride pumping system.

Download Full-text

Fatty Acid Fueled Transmembrane Chloride Transport

10.26434/chemrxiv.6823352 ◽

2019 ◽

Author(s):

Ethan N.W. Howe ◽

Philip Gale

Keyword(s):

Fatty Acids ◽

Fatty Acid ◽

Lipid Bilayer ◽

Chloride Transport ◽

Bilayer Membrane ◽

Lipid Bilayer Membrane ◽

Ph Gradient ◽

Pumping System ◽

Membrane Addition

We report an example of the use of fatty acids to drive chloride transport by creating a pH gradient across a vesicular lipid bilayer membrane. Addition of an unselective squaramide-based chloride transporter (which transports both H+and Cl-) facilitates the transport of HCl from the vesicle (driven by the pH gradient) so creating a chloride gradient. Addition of further aliquots of fatty acid ‘fuel’ can initiate further transport of chloride out of the vesicle by re-establishing the pH gradient. This is an example of a prototypical chloride pumping system.

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Effects of fatty acid from deep-sea microorganisms on lipid bilayer membrane fluidity under high pressure: comparison of branched-chain and polyunsaturated fatty acid

E3S Web of Conferences ◽

10.1051/e3sconf/202132201019 ◽

2021 ◽

Vol 322 ◽

pp. 01019

Author(s):

Kentaro Miura ◽

H. Ueno ◽

Yu Numa ◽

S. Morita ◽

Makoto Nishimoto

Keyword(s):

High Pressure ◽

Fatty Acid ◽

Lipid Bilayer ◽

Polyunsaturated Fatty Acid ◽

Fluorescence Anisotropy ◽

Rotational Diffusion ◽

Bilayer Membrane ◽

Lipid Bilayer Membrane ◽

Molecular Movement ◽

Branched Chain

In this study, the effects of unsaturated and saturated branchedchain fatty acids in biomembranes of microorganisms living under high temperature and high pressure on the fluidity of biomembranes were investigated by time-resolved fluorescence anisotropy measurements. First, the relationship between the order parameter S and the rotational diffusion coefficient Dw, which can be calculated from the fluorescence anisotropy measurements, and the motion of lipid molecules was investigated using lipids with three different structures, and it was found that the former was related to the spacing of lipid molecules and the latter to the motion of lipid molecules. Next, we investigated the S and Dw values of lipid bilayer membrane containing the saturated branched-chain fatty acid 12-methyltridecanoic acid (12-MTA) and the polyunsaturated fatty acid (PUFA) cis-4,7,10,13,16,19-docosahexaenoic acid (DHA). The results showed that 12-MTA increased the S value and decreased the Dw value. On the other hand, DHA tended to reduce the S value and increase the Dw value, albeit slightly. These results mean that 12-MTA narrows the molecular spacing of lipids and inhibits lipid molecular movement, while DHA tends to widen the molecular spacing of lipids and promote lipid molecular movement.

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Evaluation of 1-Naphthol as a Convenient Fluorescent Probe for Monitoring Ethanol-induced Interdigitation in Lipid Bilayer Membrane¶

Photochemistry and Photobiology ◽

10.1562/0031-8655(2001)073<0573:eonaac>2.0.co;2 ◽

2001 ◽

Vol 73 (6) ◽

pp. 573 ◽

Cited By ~ 12

Author(s):

N. Pappayee ◽

Ashok K. Mishra

Keyword(s):

Fluorescent Probe ◽

Lipid Bilayer ◽

Bilayer Membrane ◽

Lipid Bilayer Membrane

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Transmembrane signal transduction by cofactor transport

Chemical Science ◽

10.1039/d1sc03910e ◽

2021 ◽

Author(s):

Istvan Kocsis ◽

Yudi Ding ◽

Nicholas H. Williams ◽

Christopher A. Hunter

Keyword(s):

Signal Transduction ◽

Lipid Bilayer ◽

Metal Ion ◽

Bilayer Membrane ◽

Ester Hydrolysis ◽

Lipid Bilayer Membrane ◽

Metal Ion Cofactors

Synthetic transducers transport externally added metal ion cofactors across the lipid bilayer membrane of vesicles to trigger catalysis of ester hydrolysis in the inner compartment. Signal transduction activity is modulated by hydrazone formation.

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Protein Sensing Device with Multi-Recognition Ability Composed of Self-Organized Glycopeptide Bundle

International Journal of Molecular Sciences ◽

10.3390/ijms22010366 ◽

2020 ◽

Vol 22 (1) ◽

pp. 366

Author(s):

Mao Arai ◽

Tomohiro Miura ◽

Yuriko Ito ◽

Takatoshi Kinoshita ◽

Masahiro Higuchi

Keyword(s):

Binding Site ◽

Lipid Bilayer ◽

Structural Changes ◽

Bilayer Membrane ◽

Lipid Bilayer Membrane ◽

Target Protein ◽

Self Organization ◽

Specific Response ◽

Target Proteins ◽

Recognition Ability

We designed and synthesized amphiphilic glycopeptides with glucose or galactose at the C-terminals. We observed the protein-induced structural changes of the amphiphilic glycopeptide assembly in the lipid bilayer membrane using transmission electron microscopy (TEM) and Fourier transform infrared reflection-absorption spectra (FTIR-RAS) measurements. The glycopeptides re-arranged to form a bundle that acted as an ion channel due to the interaction among the target protein and the terminal sugar groups of the glycopeptides. The bundle in the lipid bilayer membrane was fixed on a gold-deposited quartz crystal microbalance (QCM) electrode by the membrane fusion method. The protein-induced re-arrangement of the terminal sugar groups formed a binding site that acted as a receptor, and the re-binding of the target protein to the binding site induced the closing of the channel. We monitored the detection of target proteins by the changes of the electrochemical properties of the membrane. The response current of the membrane induced by the target protein recognition was expressed by an equivalent circuit consisting of resistors and capacitors when a triangular voltage was applied. We used peanut lectin (PNA) and concanavalin A (ConA) as target proteins. The sensing membrane induced by PNA shows the specific response to PNA, and the ConA-induced membrane responded selectively to ConA. Furthermore, PNA-induced sensing membranes showed relatively low recognition ability for lectin from Ricinus Agglutinin (RCA120) and mushroom lectin (ABA), which have galactose binding sites. The protein-induced self-organization formed the spatial arrangement of the sugar chains specific to the binding site of the target protein. These findings demonstrate the possibility of fabricating a sensing device with multi-recognition ability that can recognize proteins even if the structure is unknown, by the protein-induced self-organization process.

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