Allelopathic and Medicinal plant. 26. Millettia speciosa

We grew cucumber in pots in greenhouse for 9-successive cropping cycles and analyzed the rhizosphere Pseudomonas spp. community structure and abundance by PCR-denaturing gradient gel electrophoresis and quantitative PCR. Results showed that continuous monocropping changed the cucumber rhizosphere Pseudomonas spp. community. The number of DGGE bands, Shannon-Wiener index and Evenness index decreased during the 3rd cropping and thereafter, increased up to the 7th cropping, however, however, afterwards they decreased again. The abundance of Pseudomonas spp. increased up to the 5th successive cropping and then decreased gradually. These findings indicated that the structure and abundance of Pseudomonas spp. community changed with long-term cucumber monocropping, which might be linked to soil sickness caused by its continuous monocropping.

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Dynamics of soil Trichoderma spp. communities in cucumber monocropping system

Allelopathy Journal ◽

10.26651/allelo.j/2021-52-1-1307 ◽

2021 ◽

Vol 52 (1) ◽

pp. 65-72

Author(s):

J.Y. Zhang ◽

Y.L. Guan ◽

L.Y. Ran ◽

J.F. Li ◽

W.Q. Ge ◽

...

Keyword(s):

Community Structure ◽

Quantitative Pcr ◽

Gel Electrophoresis ◽

Denaturing Gradient Gel Electrophoresis ◽

Wiener Index ◽

Trichoderma Spp ◽

Soil Sickness ◽

Evenness Index ◽

Gradient Gel Electrophoresis

We monitored the dynamics of Trichoderma spp. communities in a cucumber monocropping system. Trichoderma spp. community structure and abundance were analyzed with PCR-denaturing gradient gel electrophoresis and quantitative PCR, respectively. Results showed that long-term monocropping did not affect the Trichoderma spp. community structure as indicated by the number of bands, Shannon-Wiener index and evenness index of the PCR-denaturing gradient gel electrophoresis profile. Trichoderma spp. community structure abundance was the highest in the first cropping of cucumber. Our results suggested that changes in Trichoderma spp. communities may not be the causal agent of soil sickness in cucumber monocropping.

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Bacterial Community Structure Change Induced by Gamma Irradiation in Hydrocarbon Contaminated and Uncontaminated Soils Revealed by PCR-Denaturing Gradient Gel Electrophoresis

Biotechnology(Faisalabad) ◽

10.3923/biotech.2009.78.85 ◽

2008 ◽

Vol 8 (1) ◽

pp. 78-85 ◽

Cited By ~ 12

Author(s):

Wael S. El-Saye ◽

S. Ghanem

Keyword(s):

Community Structure ◽

Bacterial Community ◽

Gamma Irradiation ◽

Gel Electrophoresis ◽

Bacterial Community Structure ◽

Denaturing Gradient Gel Electrophoresis ◽

Structure Change ◽

Gradient Gel Electrophoresis

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Analysis of bacterial and fungal community structure in replant strawberry rhizosphere soil with denaturing gradient gel electrophoresis

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb12.300 ◽

2012 ◽

Vol 11 (49) ◽

Cited By ~ 1

Author(s):

Anying LI

Keyword(s):

Community Structure ◽

Fungal Community ◽

Gel Electrophoresis ◽

Rhizosphere Soil ◽

Denaturing Gradient Gel Electrophoresis ◽

Fungal Community Structure ◽

Gradient Gel Electrophoresis

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Effects of Long-Term Elevated CO2 on Rhizosphere and Bulk Soil Bacterial Community Structure in Pinus sylvestriformis Seedlings Fields

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.343-344.351 ◽

2011 ◽

Vol 343-344 ◽

pp. 351-356

Author(s):

Xia Jia ◽

Chun Juan Zhou

Keyword(s):

Community Structure ◽

Bacterial Community ◽

Bacterial Communities ◽

Bacterial Community Structure ◽

Denaturing Gradient Gel Electrophoresis ◽

Pinus Sylvestriformis ◽

Soil Bacterial Community Structure ◽

Gradient Gel Electrophoresis ◽

Polymerase Chain

The effect of long-term elevated CO2(as open top chambers) on rhizosphere and bulk bacterial community structure in Pinus sylvestriformis seedlings field was investigated in July, August, and September. The bacterial communities were processed by Denaturing Gradient Gel Electrophoresis (DGGE) analysis of bacterial 16S rDNA fragments amplified by PCR (Polymerase Chain Reaction) from DNA extracted directly from soil. DGGE profiles from rhizosphere samples showed large changes in rhizosphere bacterial community under elevated CO2compared to ambient except for that in September. For bulk samples, bacterial community structure changed when exposed to elevated CO2in three months. With the exception of bulk samples in August, a similitude of bacterial communities structures existed between different elevated CO2concentrations by analyzing UPGMA dendrogram based on Jaccard’s coefficient.

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Denaturing gradient gel electrophoresis analysis to study bacterial community structure in pockets of periodontitis patients

Oral Microbiology and Immunology ◽

10.1034/j.1399-302x.2003.180110.x ◽

2003 ◽

Vol 18 (1) ◽

pp. 59-65 ◽

Cited By ~ 48

Author(s):

V. Zijnge ◽

H. J. M. Harmsen ◽

J. W. Kleinfelder ◽

M. E. Van Der Rest ◽

J. E. Degener ◽

...

Keyword(s):

Community Structure ◽

Bacterial Community ◽

Gel Electrophoresis ◽

Bacterial Community Structure ◽

Denaturing Gradient Gel Electrophoresis ◽

Gradient Gel Electrophoresis

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Spatial variation in microbial community structure, richness, and diversity in an alluvial aquifer

Canadian Journal of Microbiology ◽

10.1139/w2012-087 ◽

2012 ◽

Vol 58 (9) ◽

pp. 1135-1151 ◽

Cited By ~ 15

Author(s):

P.G. Medihala ◽

J.R. Lawrence ◽

G.D.W. Swerhone ◽

D.R. Korber

Keyword(s):

Community Structure ◽

Microbial Community ◽

Spatial Variability ◽

Microbial Communities ◽

Microbial Community Structure ◽

Gel Electrophoresis ◽

Denaturing Gradient Gel Electrophoresis ◽

Microbial Community Composition ◽

Alluvial Aquifer ◽

Gradient Gel Electrophoresis

Relatively little is known regarding the spatial variability of microbial communities in aquifers where well fouling is an issue. In this study 2 water wells were installed in an alluvial aquifer located adjacent to the North Saskatchewan River and an associated piezometer network developed to facilitate the study of microbial community structure, richness, and diversity. Carbon utilization data analysis revealed reduced microbial activity in waters collected close to the wells. Functional PCR and quantitative PCR analysis indicated spatial variability in the potential for iron-, sulphate-, and nitrate-reducing activity at all locations in the aquifer. Denaturing gradient gel electrophoresis analysis of aquifer water samples using principal components analyses indicated that the microbial community composition was spatially variable, and denaturing gradient gel electrophoresis sequence analysis revealed that bacteria belonging to the genera Acidovorax , Rhodobacter , and Sulfuricurvum were common throughout the aquifer. Shannon’s richness (H′) and Pielou’s evenness (J′) indices revealed a varied microbial diversity (H′ = 1.488–2.274) and an even distribution of microbial communities within the aquifer (J′ = 0.811–0.917). Overall, these analyses revealed that the aquifer’s microbial community varied spatially in terms of composition, richness, and metabolic activity. Such information may facilitate the diagnosis, prevention, and management of fouling.

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Community Structure of Ammonia-Oxidizing Archaea and Ammonia-Oxidizing Bacteria in Soil Treated with the Insecticide Imidacloprid

BioMed Research International ◽

10.1155/2015/582938 ◽

2015 ◽

Vol 2015 ◽

pp. 1-12 ◽

Cited By ~ 6

Author(s):

Mariusz Cycoń ◽

Zofia Piotrowska-Seget

Keyword(s):

Community Structure ◽

Denaturing Gradient Gel Electrophoresis ◽

Wiener Index ◽

Nitrification Rate ◽

Ammonia Oxidizing Bacteria ◽

Community Members ◽

Insecticide Treatment ◽

Soil Nitrification ◽

Ammonia Oxidizing Archaea ◽

Gradient Gel Electrophoresis

The purpose of this experiment was to assess the effect of imidacloprid on the community structure of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in soil using the denaturing gradient gel electrophoresis (DGGE) approach. Analysis showed that AOA and AOB community members were affected by the insecticide treatment. However, the calculation of the richness (S) and the Shannon-Wiener index (H) values for soil treated with the field rate (FR) dosage of imidacloprid (1 mg/kg soil) showed no changes in measured indices for the AOA and AOB community members. In turn, the10*FRdosage of insecticide (10 mg/kg soil) negatively affected the AOA community, which was confirmed by the decrease of theSandHvalues in comparison with the values obtained for the control soil. In the case of AOB community, an initial decline followed by the increase of theSandHvalues was obtained. Imidacloprid decreased the nitrification rate while the ammonification process was stimulated by the addition of imidacloprid. Changes in the community structure of AOA and AOB could be due to an increase in the concentration of N-NH4+, known as the most important factor which determines the contribution of these microorganisms to soil nitrification.

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